The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of a Two-Week-Oral-Toxicity Study of Bombyx batryticatus L. Extracts on C57BL/6 Mice

백강잠 추출물의 C57BL/6 마우스를 이용한 2주경구투여 독성시험

  • Nam, Hyeon-Hwa (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong-Cheol (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ji-Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Yong-Bum (Korea Institute of Toxicology, Toxicopathology Center) ;
  • Kim, Joong-Sun (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 남현화 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 이지혜 (한국한의학연구원 한약자원연구센터) ;
  • 김용범 (안전성평가연구소) ;
  • 김중선 (한국한의학연구원 한약자원연구센터)
  • Received : 2020.01.30
  • Accepted : 2020.03.25
  • Published : 2020.03.30
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Abstract

Objectives : Bombyx batryticatus L. is the dried larval form of the silkworm (Bombyx mori L.) infected by Beauveria bassiania (Bals.) Vuill. It is used as a food and medicinal resource to treat asthma, headaches, epilepsy, and convulsions in traditional Korean and Chinese medicines. However, the research of the toxicity about B. batryticatus is not enough yet. Here, we investigate the effects of potential subacute toxicity following the repeated oral administration of B. batryticatus water extract to C57BL/6 mice, at various doses of 0, 50, 150, and 450 mg/kg/day during a two-week period. Methods : The following parameters were examined during the study period: body weight, gross findings, clinical signs, organ weight, hematology, serum biochemistry, histopathology, and mortality. At the end of the treatment period, all the mice were euthanized. Results : No changes were observed in the body weights, gross findings, clinical signs, organ weights, and mortality after two weeks of administration of the B. batryticatus extract. In addition, compared with the normal control group, no noticeable treatment-related changes were observed in the hematological, serum biochemical, and histopathological parameters in the treated group following treatment with doses of up to 450 mg/kg/day. Conclusion : Based on these findings, we conclude that the treatment of mice with the water extract of B. batryticatus did not cause considerable C57BL/6 toxicity, and therefore, it could be considered safe for further pharmacological studies.

Keywords

References

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