DOI QR코드

DOI QR Code

Effects of a Two-Week-Oral-Toxicity Study of Bombyx batryticatus L. Extracts on C57BL/6 Mice

백강잠 추출물의 C57BL/6 마우스를 이용한 2주경구투여 독성시험

  • Nam, Hyeon-Hwa (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong-Cheol (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ji-Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Yong-Bum (Korea Institute of Toxicology, Toxicopathology Center) ;
  • Kim, Joong-Sun (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 남현화 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 이지혜 (한국한의학연구원 한약자원연구센터) ;
  • 김용범 (안전성평가연구소) ;
  • 김중선 (한국한의학연구원 한약자원연구센터)
  • Received : 2020.01.30
  • Accepted : 2020.03.25
  • Published : 2020.03.30

Abstract

Objectives : Bombyx batryticatus L. is the dried larval form of the silkworm (Bombyx mori L.) infected by Beauveria bassiania (Bals.) Vuill. It is used as a food and medicinal resource to treat asthma, headaches, epilepsy, and convulsions in traditional Korean and Chinese medicines. However, the research of the toxicity about B. batryticatus is not enough yet. Here, we investigate the effects of potential subacute toxicity following the repeated oral administration of B. batryticatus water extract to C57BL/6 mice, at various doses of 0, 50, 150, and 450 mg/kg/day during a two-week period. Methods : The following parameters were examined during the study period: body weight, gross findings, clinical signs, organ weight, hematology, serum biochemistry, histopathology, and mortality. At the end of the treatment period, all the mice were euthanized. Results : No changes were observed in the body weights, gross findings, clinical signs, organ weights, and mortality after two weeks of administration of the B. batryticatus extract. In addition, compared with the normal control group, no noticeable treatment-related changes were observed in the hematological, serum biochemical, and histopathological parameters in the treated group following treatment with doses of up to 450 mg/kg/day. Conclusion : Based on these findings, we conclude that the treatment of mice with the water extract of B. batryticatus did not cause considerable C57BL/6 toxicity, and therefore, it could be considered safe for further pharmacological studies.

Keywords

References

  1. Heo JC, Park JY, Hwang JS, Park HC, Kang SW, Hwang SJ, Yun CY, Kwon TK, Lee SG. Comparison of in viro Antioxidant activity and Cyclooxygenase-2 Promoter Inhibitory Activity in Harmonia axyridis Pallas and Coccinella septempunctata Linne. Korean. J. Food. Preserv. 2006 ; 13(4) : 513-8.
  2. van Huis A. Potential of insects as food and feed in assuring food security. Annu Rev Entomol. 2013 ; 58 : 563-83. https://doi.org/10.1146/annurev-ento-120811-153704
  3. Jung JH, Lim BG, Bae, SJ. Study on Consumers' Perception of Edible Insect Foods. J. Korean Soc. Food Cult. 2018 ; 33(6) : 558-66. https://doi.org/10.7318/KJFC/2018.33.6.558
  4. Kong Y, Xu C, He ZL, Zhou QM, Wang JB, Li ZY, Ming X. A novel peptide inhibitor of platelet aggregation from stiff silkworm, Bombyx batryticatus. Peptides. 2014 ; 53 : 70-8. https://doi.org/10.1016/j.peptides.2013.12.004
  5. Kikuchi H, Takahashi N, Oshima Y. Novel aromatics bearing 4-O-methylglucose unit isolated from the oriental crude drug Bombyx Batryticatus. Tetrahedron Letters. 2004 ; 45(2) : 367-70. https://doi.org/10.1016/j.tetlet.2003.10.159
  6. K-herb network in Oriental Medicine Advanced Serching Integrated System (OASIS). Avaliable from : https://oasis.kiom.re.kr/kmedi/main.jsp.
  7. Hu M, Yu Z, Wang J, Fan W, Liu Y, Li J, Xiao H, Li Y, Peng W, Wu C. Traditional Uses, Origins, Chemistry and Pharmacology of Bombyx batryticatus: A Review. Molecules. 2017 ; 22(10) : 1779. https://doi.org/10.3390/molecules22101779
  8. Wu WP, Cao J, Wu JY, Chen H, Wang D. Anticancer activity of Bombyx batryticatus ethanol extract against the human tumor cell line HeLa. Genet Mol Res. 2015 ; 14(1) : 79-88. https://doi.org/10.4238/2015.January.15.10
  9. Wu JY, Sheikho A, Ma H, Li TC, Zhao YQ, Zhang YL, Wang D. Molecular mechanisms of Bombyx batryticatus ethanol extract inducing gastric cancer SGC-7901 cells apoptosis. Cytotechnology. 2017 ; 69(6) : 875-83. https://doi.org/10.1007/s10616-017-0102-7
  10. Lim HS, Kim JS, Moon BC, Ryu SM, Lee J, Park G. Batryticatus Bombyx Protects Dopaminergic Neurons Against MPTP-Induced Neurotoxicity by Inhibiting Oxidative Damage. Antioxidants (Basel). 2019 ; 8(12) : E574. https://doi.org/10.3390/antiox8120574
  11. Kleiner DE, Chalasani NP, Lee WM, Fontana RJ, Bonkovsky HL, Watkins PB, Hayashi PH, Davern TJ, Navarro V, Reddy R, Talwalkar JA, Stolz A, Gu J, Barnhart H, Hoofnagle JH; Drug-Induced Liver Injury Network (DILIN). Hepatic histological findings in suspected drug-induced liver injury: systematic evaluation and clinical associations. Hepatology. 2014 ; 59(2) : 667-70.
  12. Park GC, Chung CH, Cho JH, Hwang JS. Relationships among consumer's dietary lifestyle, image, intention to use, and willingness to pay in the context of seasoning foods of edible insects. Korean Journal of Hotel Administration. 2019 ; 119 : 281-92.
  13. Kim WJ, Yang SG, Noh PR, Park IK, Choi GY, Song JH, Moon BC. Development SCAR marker for the rapid authenticaton of Batryticatus BomByx based on COI Sequences. Kor. J. Herbol. 2019 ; 34(5) : 13-20. https://doi.org/10.6116/KJH.2019.34.5.13
  14. Kim HH, Ahn SH, Park SY. Effects of Bombycis corpus on Male Osteoporosis. J physiol & Pathol Korean Med. 2019 ; 33(1) : 56-62. https://doi.org/10.15188/kjopp.2019.02.33.1.56
  15. Lim HS, Seo YS, Ryu SM, Moon BC, Choi G, Kim JS. Two-Week Repeated Oral Dose Toxicity Study of Mantidis Ootheca Water Extract in C57BL/6 Mice. Evid Based Complement Alternat Med. 2019 ; 6 : 6180236.
  16. Kim JK, Lee IC, Kim SH, Baek HS, Bae JS, Song SW, Kim JC, Chung YH. 2-Week repeated oral dose toxicity study of 1,4-dichlorobutane in rats. J Korean Soc Occup Environ Hyg. 2013 ; 23(1) : 1-10.
  17. Schreyer SA, Wilson DL, LeBoeuf RC. C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. 1998 ; 136(1) : 17-24. https://doi.org/10.1016/S0021-9150(97)00165-2
  18. Huang X, Lee RJ, Qi Y, Li Y, Lu J, Meng Q, Teng L, Xie J. Microfluidic hydrodynamic focusing synthesis of polymer-lipid nanoparticles for siRNA delivery. Oncotarget. 2017 ; 8(57) : 96826-96836. https://doi.org/10.18632/oncotarget.18281