대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
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오가피청혈플러스가 NF-κB 및 MAPKs 신호전달체계와 혈관부착인자 등에 미치는 영향

The Effect of Acanthopanax sessiliflorum Cheonghyeol Plus on NF-κB and MAPKs Signaling and Vascular Attachment Factors

  • 이지원 (대전자생한방병원 한방내과) ;
  • 최규철 (대전자생한방병원 한방내과) ;
  • 배지은 (대전자생한방병원 한방내과) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실)
  • Lee, Ji-won (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine) ;
  • Choi, Gyu-cheol (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine) ;
  • Bae, Ji-eun (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine) ;
  • Yoo, Ho-ryong (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Kim, Yoon-sik (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Seol, In-chan (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University)
  • 투고 : 2020.12.01
  • 심사 : 2020.12.31
  • 발행 : 2020.12.30
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초록

Objective: This study was performed to investigate the effect of Acanthopanax sessiliflorum Cheonghyeol plus (ASCP) on NF-κB and MAPK signaling and vascular adhesion factors associated with dyslipidemia in human umbilical vein endothelial cells (HUVECs). Methods: We measured the scavenging activity of DPPH radical and ABTS radical by ASCP in HUVECs. We measured the protein expression levels of NF-κB, IκBα, ERK, JNK, and p38 after treatment of HUVECs with TNF-α. We measured the expression levels of MCP-1, ICAM-1, and VCAM-1 mRNA and of MCP-1, ICAM-1, and VCAM-1 biomarkers after treatment of HUVECs with TNF-α. Results: The DPPH and ABTS radical scavenging activity of ASCP increased in a concentration-dependent manner. NF-κB, IκB, ERK, p38 protein expression levels decreased following ASCP treatment at all concentrations compared to untreated control HUVECs. JNK protein expression levels decreased in ASCP-treated HUVECs compared to untreated controls at concentrations of 100 ㎍/mL. MCP-1 mRNA expression level decreased with ASCP treatment ≥200 ㎍/mL compared to the control. ICAM-1 and VCAM-1 mRNA expression levels decreased at all concentrations compared to the control. MCP-1 protein expression level was reduced compared to the control at concentrations ≥200 ㎍/mL, ICAM-1 protein expression level was reduced compared to the control at concentrations ≥100 ㎍/mL, and VCAM-1 protein expression level was reduced at all concentrations. Conclusions: These results suggest that ASCP has an antioxidative and hypolipidemic effect and that ASCP could treat and prevent dyslipidemia, atherosclerosis, and cardio-cerebrovascular diseases.

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참고문헌

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