Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of phenolics from Oplismenus undulatifolius in α-MSH-stimulated B16F10 melanoma cells

  • Park, Hye-Jin (School of Food science & Biotechnology, Kyungpook National University) ;
  • Lee, Eun-Ho (School of Food science & Biotechnology, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Cho, Young-Je (School of Food science & Biotechnology, Kyungpook National University)
  • Received : 2020.02.03
  • Accepted : 2020.03.06
  • Published : 2020.03.31
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Abstract

In this study, the efficacy of melanoma cell B16F10 was investigated using the Korean native plant Oplismenus undulatifolius (OU). First, the cell viability of the extract was more than 90% when treated with 15 ㎍/mL of phenolics from OU. The results showed that melanin biosynthesis and cellular tyrosinase synthesis were inhibited by treatment with α-melanocyte-stimulating hormone-stimulated mouse melanoma cell B16F10 at a concentration of 15 ㎍/mL of phenolics for cell-line efficacy. The expression of tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia transcription factor (MITF) protein was confirmed by western blot to investigate the effect of phenolics from OU on melanin biosynthesis. When treated with phenolics from OU 15 ㎍/mL, tyrosinase, TRP-1, TRP-2, and MITF decreased the protein expression level. In particular, tyrosinase, TRP-1, and MITF inhibited the production amount to a level similar to that of the non-treated normal group, indicating that the effect was excellent. Therefore, phenolics from OU acts as an inhibitor of tyrosinase, TRP-1, TRP-2, and its transcription factor MITF, and participates in melanin biosynthesis mechanism. These results suggested the potential for development as a material.

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References

  1. Korea forest seed and variety center (KFSV) (2009) Life characteristics of plant resources from Korea forest. Chungju
  2. Lee EH, Kim MU, Kang IK, Park KI, Cho YJ (2018) Inhibitory activities of extracts from Hypericum ascyron L. on biological enzymes. J Korean Soc Food Sci Nutr 47: 7-14 https://doi.org/10.3746/jkfn.2018.47.1.007
  3. Park SY, Song IH, Cho YJ (2018) Elicitor treatment potentiates the preventive effect of Saururus chinensis leaves on stress-induced gastritis. Appl Biol Chem 61: 423-431 https://doi.org/10.1007/s13765-018-0375-3
  4. Lee TB (1999) Illustrated flora of Korea. Hyangmunsa, Seoul
  5. Ohmoto T, Ikuse M (1970) Triterpenoids of the gramineae. Phytochem 9: 2137-2148 https://doi.org/10.1016/S0031-9422(00)85379-0
  6. Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Frdd Rad Biol Med 20: 933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  7. Kinsella JE, Frankel E, German B, Kanner J (1993) Possible mechanism for the protective role of antioxidants in wine and plant foods. Food Technol 47: 85-89
  8. Lee YS, Yoon HK, Kim NW (2010) The physiological activities of ripe fruit of Poncirus trifoliata. Kor J Food Preserv 17: 698-705
  9. Ju HJ, Lee SA, Kim RH, Park BD, Kim GN (2017) Evaluation of Curcuma longa L. water extracts as beauty food materials in B16F10 and human skin fibroblasts. Asian J Beauty Cosmetol 15: 214-222 https://doi.org/10.20402/ajbc.2016.0130
  10. Urabe K, Aroca P, Tsukamoto K, Mascagna D, Palumbo A, Prota G, Hearing VJ (1994) The inherent cytotoxicity of melanin precursors: a revision. Biochimica et Biophysica Acta 1221: 272-278 https://doi.org/10.1016/0167-4889(94)90250-X
  11. Lin CB, Babiarz L, Liebel F, Price ER, Kizoulis M, Gendimenico GJ, Seiberg M (2002) Modulation of microphthalmia-associated transcription factor gene expression alters skin pigmentation. Journal of investigative dermatology 119: 1330-1340 https://doi.org/10.1046/j.1523-1747.2002.19615.x
  12. Lee EH, Kim BO, Cho YJ (2017) Inhibitory activities on biological enzymes of extracts from Oplismenus undulatifolius. J Appl Biol Chem 60(2): 101-108 https://doi.org/10.3839/jabc.2017.017
  13. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosen- sitivity testing. Cancer Res 47: 936-942
  14. Hosoi J, Abe E, Suda T, Kuroki T (1985) Regulation of melanin synthesis of B16 melanoma cell by 1 alpha, 25-dihydroxyvitamin $D_{3}$ and retinoic acid. Cancer Res 45: 1474-1478
  15. Tsareva SA, Moriggl R, Corvinus FM, Wiederanders B, Schutz A, Kovacic B, Friedrich K (2007) Signal transducer and activator of transcription 3 activation promotes invasive growth of colon carcinomas through matrix metalloproteinase induction. Neoplasia 9: 279-291 https://doi.org/10.1593/neo.06820
  16. Kobayashi T, Imokawa G, Bennett DC, Hearing VJ, (1998) Tyrosinase stabilization by Tyrp1 (the brown Locus Protein). J Biol Chem 273: 31801-31805 https://doi.org/10.1074/jbc.273.48.31801
  17. Friedman PS, Wren F, Buffey J, Macnele S (1990) ${\alpha}$-MSH causes a small rise in cAMP but has no effect on basal or ultra violet-stimulated melanogenesis in human melanocytes. Br J Dermatol 123: 145-151 https://doi.org/10.1111/j.1365-2133.1990.tb01841.x