한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제48권1호
  • /
  • Pages.32-37
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  • 2020
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Improvement of a Unified Saccharification and Fermentation System for Agaro-bioethanol Production in Yeast

  • Lee, So-Eun (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University) ;
  • Kim, Yeon-Hee (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University)
  • 투고 : 2019.09.17
  • 심사 : 2019.10.29
  • 발행 : 2020.03.28
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초록

We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.

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참고문헌

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