Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mec1 Modulates Interhomolog Crossover and Interplays with Tel1 at Post Double-Strand Break Stages

  • Lee, Min-Su (Department of Life Sciences, Chung-Ang University) ;
  • Joo, Jung Whan (Department of Life Sciences, Chung-Ang University) ;
  • Choi, Hyungseok (Department of Life Sciences, Chung-Ang University) ;
  • Kang, Hyun Ah (Department of Life Sciences, Chung-Ang University) ;
  • Kim, Keunpil (Department of Life Sciences, Chung-Ang University)
  • Received : 2019.09.15
  • Accepted : 2019.12.01
  • Published : 2020.03.28
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Abstract

During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae, Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1ATM and Mec1ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1ATM and Mec1ATR in meiotic prophase via physical analysis of recombination. Tel1ATM cooperated with Mec1ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1ATM and Mec1ATR function redundantly or independently in all post-DSB stages.

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