한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제20권2호
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  • Pages.78-87
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  • 2020
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
권호 표지
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65종 한약처방이 3T3-L1 지방전구세포의 지방 분화에 미치는 효능 비교 연구

Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions

  • 최혜민 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 유병우 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 김민주 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 김정옥 (한국한의약진흥원 한의기술 R&D 2팀)
  • Choi, Hye-Min (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Yu, Byung-Woo (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Kim, Min-Ju (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Kim, Jung-Ok (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
  • 투고 : 2020.10.30
  • 심사 : 2020.12.09
  • 발행 : 2020.12.30
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초록

Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal medicine in 3T3-L1 preadipocyte cells. Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 ㎍/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 ㎍/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses. Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α. Conclusions : These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.

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참고문헌

  1. Yoo SR, Seo CS, Kim OS, Shin HK, Jeong SJ. Anti-adipogenic and antioxidanteffects of the traditional Korean herbalformula Samchulgeonbitang: an in vitro study. Int J Clin Exp Med. 2015 ; 8(6) : 8698-708.
  2. Lee JS, Lee SH. The reductive effects of oriental medicine on the body fat and abdominal obesity. J Korean Soc Study Obes. 2001 ; 1 : 33-42.
  3. Korea Centers for Disease Control and Prevention. 2009 Korea national health and nutrition and examination survey. Seoul : Ministry of Health and Welfare. 2010.
  4. Kopelman PG. Obesity as a medical problem. Nature. 2000 ; 404 : 635-43. https://doi.org/10.1038/35007508
  5. Fujioka K, Seaton TB, Rowe E, Jelinek CA, Raskin P, Levovitz HE, et al. Weight loss with sibutramine improves glycaemic control and other metabolic parameters in obese patients with type 2 diabetes mellitus. Diabetes Obes Metab. 2000 ; 2(3) : 175-87. https://doi.org/10.1046/j.1463-1326.2000.00081.x
  6. McMahon FG, Fujioka K, Singh BN, Mendel CM, Rowe E, Rolston K, et al. Efficacy and safety of sibtramine in obese white and African American patients with hypertension: a 1-year, double-blind, placebo-controlled, multicenter trial. Arch Intern Med. 2000 ; 160(14) : 2185-91. https://doi.org/10.1001/archinte.160.14.2185
  7. Sui Y, Hui HL, Wong VCW, Brown N, Li XL, Hui HLW, et al. A systematic review on use of Chinese medicine and acupuncture for treatment of obesity. Obesity Reviews. 2012 ; 13(5) : 409-30. https://doi.org/10.1111/j.1467-789X.2011.00979.x
  8. Xu L, Zhao W, Wang D, Ma X. Chinese medicine in the battle against obesity and metabolic diseases. FrontPhysiol. 2018 ; 9 : 850.
  9. Fu C, Jiang Y, Guo J, Su Z. Natural products with anti-obesity effects and different mechanisms of action. J Agric Food Chem. 2016 ; 64(51) : 9571-85. https://doi.org/10.1021/acs.jafc.6b04468
  10. Otto TC, Lane MD. Adipose development : from stem cell to adipocyte. Rev Biochem Mol Biol. 2005 ; 40 : 229-42. https://doi.org/10.1080/10409230591008189
  11. Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell. 1974 ; 3 : 127-33. https://doi.org/10.1016/0092-8674(74)90116-0
  12. Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol Rev. 1998 ; 78 : 783-809. https://doi.org/10.1152/physrev.1998.78.3.783
  13. Green H, Kehinde O. Sublines of mouse 3T3cells that accumlate lipid. Cell. 1974 ; 1 : 113-6. https://doi.org/10.1016/0092-8674(74)90126-3
  14. Rayalam S, Della-Fera MA, Baile CA. Phytochemicals and regulation of the adipocyte life cycle. J Nutritional Biochemistry. 2008 ; 19 : 717-26. https://doi.org/10.1016/j.jnutbio.2007.12.007
  15. Dave S, Kaur NJ, Nanduri R, Dkhar HK, Kumar A, Gupta P. Inhibition of adipogenesis and induction of apoptosis and lipolysis by stem bromelanin in 3T3-L1 a dipocytes. PLos One. 2012 ; 7 : e30831. https://doi.org/10.1371/journal.pone.0030831
  16. Rosen ED. The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acids. 2005 ; 73 : 31-4. https://doi.org/10.1016/j.plefa.2005.04.004
  17. Evans RM, Barish GD, Wang YX. PPARs and the complex journey to obesity. Nat Med. 2004 ; 10 : 355-61. https://doi.org/10.1038/nm1025
  18. Yoon KY. Dongui imsang bangjehak. Seoul : Myoung Bo Press. 1985 : 50.
  19. Lu HY, Cai JF, Du LB, Wang Z, Ye WW, Lou CJ. Study on improvement of Banxia Xiexin decoction against irinotecan-induced injury of stomach in nude mice bearing human small-cell lung cancer. Chinese Archives of Traditional Chinese Medicine. 2012 ; 30(6) : 1289-90.
  20. Shin MR, Seo BI, Son CG, Roh SS, An HJ. Banhasasim-tang treatment reduces the severity of esophageal mucosal ulcer on chronic acid reflux esophagitis in rats. BioMed Research International. 2017 ; 2017 : 7157212.
  21. Zhou FX, Chen YL, Pang YF, Wu YS. Effects of Banxia Xiexin decoction and its components on cell cycle and apoptosis of gastric cancer SGC-7901 cells. Chinese Journal of Experimental Traditional Medical Formulae. 2012 ; 18(23) : 203-6.
  22. Lu HY, Cai JF, Du LB, Wang Z, Ye WW, Lou CJ. Study on improvement of Banxia Xiexin decoction against irinotecan-induced injury of stomach in nude mice bearing human small-cell lung cancer. Chinese Archives of Traditional Chinese Medicine. 2012 ; 30(6) : 1289-90.
  23. Jin SE, Lim HS, Kim Y, Seo CS, Yoo SR, Shin HK, et al. Traditional herbal formula banhasasim-tang exerts anti-inflammatory effects in RAW 264.7 macrophages and HaCaT keratinocytes. Evidence Based Complementary and Alternative Medicine. 2015 ; 2015 : 728380. https://doi.org/10.1155/2015/728380
  24. Zhang F, Sun X, Qiu G, Zhou X, Liu T, Xiushen H. Study regulation of Banxiaxiexin decoction of fasting blood-glucose and gastrointestinal hormones of diabetic rats. China Press of Traditional Chinese Medicine. 2014 ; 30(1) : 8-10.
  25. Lee JY, Yoon BK. Effects of Samhwangsasim-tang on obesity-related metabolic diseaseinmice. Dept. of Korean Medicine. 2014 ; 22(1) : 93-104.
  26. Yoon HJ, Park YS. Effects of scutellariabaicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. Journal of The Korean Society of Food Science and Nutrition. 2010 ; 39(2): 219-26. https://doi.org/10.3746/JKFN.2010.39.2.219