Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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Effect of Pu'er tea extract on C2C12 myoblast differentiation

보이차 열수 추출물의 근아세포 근분화에 미치는 영향

  • Lee, Hyoseong (Department of Biochemistry, Chungnam National University) ;
  • Choi, Sunkyung (Department of Biochemistry, Chungnam National University) ;
  • Lee, Boyeong (Department of Biochemistry, Chungnam National University) ;
  • Kim, Eunmi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Lee, Woonghee (Institute of Biotechnology, Chungnam National University) ;
  • Han, Hyosang (Department of Health Administration, Joongbu University) ;
  • Kim, Keekwang (Department of Biochemistry, Chungnam National University)
  • 이효성 (충남대학교 생화학과) ;
  • 최선경 (충남대학교 생화학과) ;
  • 이보영 (충남대학교 생화학과) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 이웅희 (충남대학교 생물공학연구소) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2020.09.21
  • Accepted : 2020.12.20
  • Published : 2020.12.28
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Abstract

At present, aging-related degenerative muscle diseases are considered a serious problem. However, the drug effect on the treatment and prevention of sarcopenia has not been investigated. The purpose of this study was to evaluate the value of extract of Pu'er tea as a remedy to alleviate the symptoms of sarcopenia. Pu'er tea extracts showed excellent radical scavenging ability. The expression of Myh3 was promoted and myotube formation also was increased by treatment of Pu'er tea extracts. These results suggest that Pu'er tea is a natural substance that promotes myogenesis, and is valuable as a material for pharmaceutical research on the prevention and treatment of various muscle diseases, including sarcopenia. And It is necessary to confirm specific indicator substances of puer tea and further study on this.

현재 노화와 관련된 퇴행성 근육 질환은 심각한 문제로 간주되고 있으나 근육 감소증의 치료 및 예방에 대한 약물 효과는 충분히 연구되지 않다. 이에 중국 전통차인 보이차의 추출물을 근육 감소증의 증상을 완화하기 위한 치료제로서 가치를 평가하고자 하였다. 본 실험에서는 이를 평가하기 위하여 ABTS 분석, MTS 분석, 면역 블롯 분석, 면역 형광 현미경법을 수행하였다. 보이차 추출물은 우수한 라디칼 소거 능을 나타냈으며, 또한 근관을 형성하는 Myh3의 발현이 촉진시켰고, 근관 형성을 증진시켰다. 따라서 보이차는 근육생성을 촉진하는 천연 물질이며 근감소증을 포함한 다양한 근육 질환의 예방 및 치료에 대한 제약 연구의 재료로 가치가 있음을 시사하며, 보이차의 구체적인 지표물질을 확인하고 이에 대한 추가연구가 필요할 것으로 보인다.

Keywords

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