Journal of Applied Biological Chemistry

  • 제63권4호
  • /
  • Pages.393-399
  • /
  • 2020
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지
DOI QR코드

DOI QR Code

방풍, 식방풍의 대사체 프로파일링을 통한 지표성분 선정 및 분석법검증

Metabolic profiling and method validation of marker compounds from Saposhnikoviae Radix and Peucedani Japonici Radix

  • Choi, Bo-Ram (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Dahye (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Han, Kyung-Sook (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Doo Jin (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyun, Do Yoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
  • 투고 : 2020.10.25
  • 심사 : 2020.11.01
  • 발행 : 2020.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

방풍(Saposhnikoviae Radix, SR) 과 식방풍(Peucedani Japonici Radix, PR)은 각각 다른 기원식물을 가지며 속과 종이 다르지만 한국, 중국, 및 일본에서 대표적인 전통 한약재로 오랫동안 혼용되어 사용되었다. 본 연구에서는 방풍과 식방풍의 판별 마커를 확인하기 위하여 UPLC-QTOF/MS를 이용한 대사체 프로파일링 및 다변량 통계분석을 진행하였다. 그 결과, 5-O-methylvisammioside와 peucedanol을 각각 방풍과 식방풍의 지표성분으로 선정하였으며, UPLC를 이용하여 분석법을 검증하였다. 제안된 방풍 및 식방풍의 지표성분에 대한 분석법 검증은 방풍과 식방풍의 분류와 품질 평가 및 성분 검증을 위한 효과적인 방법이 될 것으로 기대된다.

Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.

키워드

참고문헌

  1. Kim MK, Yang DH, Jung M, Jung EH, Eom HY, Suh JH, Min JW, Kim U, Min H, Kim J, Han SB (2011) Simultaneous determination of chromones and coumarins in Radix Saposhnikoviae by high performance liquid chromatography with diode array and tandem mass detectors. J Chromatogr A 1218: 6319-6330 https://doi.org/10.1016/j.chroma.2011.06.103
  2. Kim SJ, Chin YW, Yoon KD, Ryu MY, Yang MH, Lee JH, Kim J (2008) Chemical Constituents of Saposhnikovia divaricata. Kor J Pharmacogn 39: 357-364
  3. Jung JK, Park YK (2012) Effects of Saposhnikoviae Radix on allergic responses in OVA-induced Allergic rhinitis mice. Kor J Herbology 27: 85-91 https://doi.org/10.6116/kjh.2012.27.5.85
  4. Choi CH, Wang KT, Cho HR, Jeong JG, Jeong HW (2014) Inhibitory Effects of Saposhnikoviae Radix Extracts on the Melanin Production and Elastase Activity in B16F10 cells. Korean J Oriental Physiology & Pathology 28: 296-302
  5. Chun JM, Kim HS, Lee AY, Kim SH, Kim HK (2016) Anti-Inflammatory and Antiosteoarthritis Effects of Saposhnikovia divaricata ethanol Extract: In Vitro and In Vivo Studies. Evidence-Based Complementary and Alternative Medicine 2016: 1984238
  6. Khan S, Kim YS (2013) Molecular mechanism of inflammatory signaling and predominant role of Saposhnikovia divaricata as anti-inflammatory potential. Nat Prod Sci 19: 120-126
  7. Kang J, Zhou L, Sun JH, Ye M, Han J, Wang BR, Guo DA (2008) Three new compounds from the roots of Saposhnikovia divaricata. J Asian Nat Prod Res 10: 971-976 https://doi.org/10.1080/10286020802217556
  8. Kreiner J, Pang E, Lenon GB, Yang Angela WH (2017) Saposhnikoviae divaricata: a phytochemical, pharmacological, and pharmacokinetic review. Chin J Nat Med 15: 255-264
  9. Dai J, Chen X, Cheng W, Liu X, Fan X, Shen Z, Bi Kaishun (2008) A sensitive liquid chromatography-mass spectrometry method for simultaneous determination of two active chromones from Saposhnikovia root in rat plasma and urine. J Chromatogr B 868: 13-19 https://doi.org/10.1016/j.jchromb.2008.03.031
  10. Kim SM, Shin DI, Song HS, Yoon ST (2006) Vegetation Structure of Peucedanum japonicum Thunb. Community in East Seaside of South Korea. Korean J Medicinal Crop Sci 14: 347-353
  11. Nam JY, Pyu KS (1975) Pharmacognostical studies on Korea "Bang Poong". Kor J Pharmacogn 6: 151-159
  12. Chun J, Kim J, Kim YS (2018) 3,4-Disenecioylkhellactone from Peucedanum japonicum Thunb. Induces Apoptosis Mediated by Inhibiting STAT3 Signaling in Human Gastric Cancer Cells. Kor J Pharmacogn 49: 225-230
  13. Chung SH, Kim KJ, Suh DH, Lee KS, Choi BS (1994) Changes in Growth and Yield of Peucedanum japonicum Thunberg by Planting Time, Mulching, and Planting Density. Korean Journal of Medicinal Crop Science 2: 121-126
  14. Jung HK, Sim MO, Jang JH, Kim TM, An BK, Kim MS, Jung WS (2016) Anti-obesity effects of Peucedanum japonicum Thunberg L. on 3T3-L1 cells and high-fat diet-induced obese mice. Korean J Plant Resour 29: 1-10 https://doi.org/10.7732/kjpr.2016.29.1.001
  15. Lim H, Kim I, Jeong Y (2019) Antioxidant Activities of Peucedanum japonicum Thunberg Root Extracts. J Korean Soc Food Sci Nutr 48: 32-39 https://doi.org/10.3746/jkfn.2019.48.1.032
  16. Kim KN, Choi MJ, Lee Y, Cho SH (2013) The Protective and Recovery Effects of Peucedanum Japonicum Thunberg for Vascular Dementia. J of Oriental Neuropsychiatry 24: 123-130 https://doi.org/10.7231/jon.2013.24.1.123
  17. Whang WK, Lee SJ, Kim H, Cho H, Lee KS, Kang IH, Han I (2001) Standardization of Peucedani Radix. Kor J Pharmacogn 32: 292-296
  18. Hong MJ, Kim J (2017) Determination of the Absolute Configuration of Khellactone Esters from Peucedanum japonicum Roots. J Nat Prod 80: 1354-1360 https://doi.org/10.1021/acs.jnatprod.6b00947
  19. Lee JW, Ji SH, Lee MK, Kim GS, Ahn YS, Ko HJ, Baek NI, Lee DY (2015) Metabolomics Based on UPLC-QTOF/MS Applied for the Discrimination of Cynanchum wilfordii and Cynanchum auriculatum. Metabolomics 5: 4
  20. Kim EJ, Kwon J, Park SH, Park C, Seo YB, Shin HK, Kim HK, Lee KS, Choi SY, Ryu DH, Hwang GS (2011) Metabolite Profiling of Angelica gigas from Different Geographical Origins Using 1H NMR and UPLC-MS Analyses. J Agric Food Chem 59: 8806-8815 https://doi.org/10.1021/jf2016286
  21. Yoon D, Kim S, Lee M, Yoon C, Kim S (2016) 1H-NMR-based metabolomic study on toxicity of methomyl and methidathion in fish. Journal of Environmental Science and Health, Part B 51:824-831 https://doi.org/10.1080/03601234.2016.1208460
  22. Godzien J, Ciborowski M, Angulo S, Barbas C (2013) From numbers to a biological sense: How the strategy chosen for metabolomics data treatment may affect final results. A practical example based on urine fingerprints obtained by LC-MS. Electrophoresis. 34: 2812-2826 https://doi.org/10.1002/elps.201300053
  23. Bijisma S, Bobeldijk I, Verheij ER, Ramaker R, Kochhar S, Macdonald IA, Ommen BV, Smilde AK (2006) Large-Scale Human Metabolomics Studies: A Strategy for Data (Pre-) Processing and Validation. Anal. Chem. 78: 567-574 https://doi.org/10.1021/ac051495j
  24. Yoon D, Choi BR, Ma S, Lee JW, Jo IH, Lee YS, Kim GS, Kim S, Lee DY (2019) Metabolomics for Age Discrimination of Ginseng Using a Multiplex Approach to HR-MAS NMR Spectroscopy, UPLC-QTOF/MS, and GC x GC-TOF/MS. Molecules 24: 2381 https://doi.org/10.3390/molecules24132381
  25. Lee JW, Ji SH, Choi BR, Choi DJ, Lee YG, Kim HG, Kim GS, Kim K, Lee YH, Baek NI, Lee DY (2018) UPLC-QTOF/MS-Based Metabolomics Applied for the Quality Evaluation of Four Processed Panax ginseng Products. Molecules 23: 2062 https://doi.org/10.3390/molecules23082062
  26. Lin H, Zhu H, Tan J, Wang H, Dong Q, Wu F, Liu Y, Li P, Liu J (2019) Non-Targeted Metabolomic Analysis of Methanolic Extracts of Wild-Simulated and Field-Grown American Ginseng. Molecules 24: 1053 https://doi.org/10.3390/molecules24061053
  27. Guo X, Long P, Meng Q, Ho CT, Zhang L (2018) An emerging strategy for evaluating the grades of Keemun black tea by combinatory liquid chromatography-Orbitrap mass spectrometry-based untargeted metabolomics and inhibition effects on α-glucosidase and α-amylase. Food Chemistry 246: 74-81 https://doi.org/10.1016/j.foodchem.2017.10.148
  28. Kim HS, Choi G, Lee AY (2018) Ultra-performance convergence chromatography method for the determination of four chromones and quality control of Saposhnikovia divaricata (Turcz.) Schischk. J Sep Sci 41: 1682-1690 https://doi.org/10.1002/jssc.201701281
  29. Chang CZ, Wu SC, Kwan AL, Lin CL (2015) 4-O-β-D-glucosyl-5-O-methylvisam minol, an active ingredient of Saposhnikovia divaricata, attenuates high-mobility group box 1 and subarachnoid hemorrhage-induced vasospasm in a rat model. Behav Brain Funct 11: 28 https://doi.org/10.1186/s12993-015-0074-8
  30. Shin KH, Kang SS, Choi HJ (1992) Analysis of the Coumarin Constituents in Peucedanii Radix. Kor. J. Pharmacogn 23: 20-23
  31. Son HK, Kang ST, Jung HO, Lee JJ (1992) Changes in physicochemical properties of Peucedanum japonicum Thunb. after blanching. Korean J Food Preserv 20: 628-635 https://doi.org/10.11002/kjfp.2013.20.5.628
  32. Karakaya S, Koca M, Sytar O, Dursunoglu B, Ozbek H, Duman H, Guvenalp Z, Kilic CS (2019) Antioxidant and anticholinesterase potential of Ferulago cassia with farther bio-guided isolation of active coumarin constituents. South African Journal of Botany 121: 536-542 https://doi.org/10.1016/j.sajb.2019.01.020
  33. Seo UM, Zhao BT, Kim YH, Kang JS, Son JK, Woo MH (2016) Simultaneous analysis of seven marker compounds from Saposhnikoviae Radix, Glehniae Radix and Peucedani Japonici Radix by HPLC/PDA. Arch Pharm Res 39: 695-704 https://doi.org/10.1007/s12272-016-0740-x