Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of 2,3-Butanediol Production from Red Seaweed Gelidium amansii Hydrolysates Using Engineered Saccharomyces cerevisiae

  • Ra, Chae Hun (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University) ;
  • Seo, Jin-Ho (Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University) ;
  • Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
  • Received : 2020.07.28
  • Accepted : 2020.09.18
  • Published : 2020.12.28
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Abstract

Hyper-thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150℃ for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and CTec2 at a dose of 16 U/ml led to the production of 12.0 g/l of reducing sugar with an efficiency of enzymatic saccharification of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/l of 5-hydroxymethylfurfural showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h were 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.

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References

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