Comparative Analysis of Gallic Acid Content by Chestnut Varieties

밤의 품종에 따른 Gallic acid 함량 비교분석

In this study, the gallic acid content of various varieties of chestnuts (Daebo, Okgwang, Chukpa, Samjosaeng) was investigated during the period between May 2018 to July 2019. A quantitative analysis was performed by HPLC using extracts of chestnut, inner skin, outer skin, branches, chestnut, male flower, and the male flower for each type of chestnut tree. Gallic acid was identified by dissolving standard gallic acid in water and analyzed three times in the concentration range of 100, 200, 250, and 500ppm. Linearity was confirmed by the peak area ratio at each concentration. Among the different chestnut varieties, the gallic acid content was highest at 0.0863% in Chukpa, followed by Daebo, Okgwang, and Samjosaeng. While comparing the average gallic acid content of each part of the chestnut tree, it was observed that the falling male flower had the highest content at 1.2100%, followed by chestnut leaves, chestnut pines, and branches. In a comparison of the inner skins, the Daebo variety had the highest gallic acid content at 0.7463% followed by Chukpa, Okgwang, and Samjosaeng. The outer skin of Samjosaeng had the highest content at 0.4918%, followed by Chukpa, Daebo, and Okgwang. The pines of the Samjosaeng chestnut had the highest content at 1.3035%, followed by Daebo, Chukpa, and Okgwang.

본 연구에서는 밤의 품종(대보, 옥광, 축파, 삼조생)에 따른 Gallic acid 함량을 알아보았다. 밤나무 종류별로 알밤, 내피, 외피, 가지, 밤송이, 수꽃, 낙화수꽃의 추출물을 사용하여 HPLC로 정량분석하였다. Gallic acid의 동정은 표준품인 Gallic acid를 물에 녹여 100, 200, 250, 500ppm의 농도 범위에서 3회씩 분석하였고, 각 농도에서 피크면적비로 직선성을 확인하였다. 실험 기간은 2018년 5월부터 2019년 7월까지 진행했으며 밤 품종과 부위별 Gallic acid 함량을 확인한 결과는 다음과 같다. 밤에서 Gallic acid 함량은 축파가 0.0863%로 가장 높았고, 대보, 옥광, 삼조생순이었다. 밤나무의 부위별 Gallic acid 평균 함량에서는 낙화 수꽃에서 1.2100%로 가장 높았으며, 밤나무 잎, 밤송이, 가지의 순이었다. 내피에서는 대보 품종이 0.7463%로 가장 높았고, 축파, 옥광, 삼조생 순이었다. 외피에서는 삼조생품종이 0.4918%로 가장 높았고, 축파, 대보, 옥광 순이었다. 밤송이에서는 삼조생 품종이 1.3035%로 가장 높았고 대보, 축파, 옥광 순이었다.