Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Production of monoclonal antibodies against marine birnavirus

Marine birnavirus (MABV)에 대한 단클론 항체 생산

  • Kong, Kyoung-Hui (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 공경희 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Received : 2020.11.18
  • Accepted : 2020.11.27
  • Published : 2020.12.31
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Abstract

We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.

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References

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