한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제37권6호
  • /
  • Pages.1698-1705
  • /
  • 2020
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

배양가능한 피부세균의 분리 및 동정

Isolation and identification of culturable bacteria from human skin

  • 배영민 (창원대학교 생명보건학부)
  • Bae, Young-Min (Department of Life Science and Public Health, Changwon University)
  • 투고 : 2020.10.25
  • 심사 : 2020.12.29
  • 발행 : 2020.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

나이가 20-25세인 남녀 성인들 20명(남성 5명, 여성 15명)의 엄지손가락 표면에 상재하는 세균을 채취하고 Luria-Bertani agar에서 배양하였다. 배양된 세균들의 16S rDNA를 polymerase chain reaction을 통하여 증폭하고, 그 염기서열을 분석하였다. 이렇게 얻어진 염기서열을 genbank의 자료들과 비교하여 세균들을 동정한 결과, 총 14종의 세균들이 확인되었다. 개인별로는 평균 2.5종의 배양 가능한 세균들을 손의 피부에 보유하는 것으로 확인되었다. 확인된 세균 중에서는 Staphylococcus 종들이 가장 널리 존재하고 있었으며, 다음으로는 Micrococcus luteus이었다. Staphylococcus 종들의 경우에는 그 분포에 있어서 성별에 따른 차이가 적었으나, Micrococcus luteus의 경우에는 남자보다 여자에게서 훨씬 더 많이 분리되는 경향을 보였다. 이러한 결과는 피부 질환에 대한 연구, 피부질환에 대한 치료제 개발, 피부에 사용하는 화장품개발 등의 분야에 도움이 될 것으로 사료된다.

Bacteria were collected from the thumb surface of the twenty young adults that are 20 to 25 years old and cultured on the Luria-Bertani agar. The 16S rDNA of the cultured bacteria was amplified by polymerase chain reaction(PCR) and DNA sequence of the PCR products analyzed. Total 14 different bacterial species were identified by comparing their 16S rDNA sequence with the data in genbank. It appears that each individual has 2.5 different bacterial species in average. Staphylococcal species were the most abundant among the identified bacteria and Micrococcus luteus was the second. Staphylococcal species were isolated at similar frequency between male and female donors but Micrococcus luteus was isolated more frequently from female than male donors. The result obtained in this study might be useful in research of dermatic diseases, searching for new drugs for those diseases and development of new cosmetics.

키워드

참고문헌

  1. J. Lehtimaki, A. Karkman, T. Laatikainen, L. Paalanen, L. von Hertzen, T. Haahtela, I. Hanski, L. Ruokolainen, "Patterns in the skin microbiota differ in children and teenagers between rural and urban environments" Sci Rep, Vol.7, pp. 45651, (2017). https://doi.org/10.1038/srep45651
  2. S. Ying, D. N. Zeng, L. Chi, Y. Tan, C. Galzote, C. Cardona, S. Lax, J. Gilbert, Z. X. Quan, "The Influence of Age and Gender on Skin-Associated Microbial Communities in Urban and Rural Human Populations", PLoS One, Vol.10, pp. e0141842, (2015). https://doi.org/10.1371/journal.pone.0141842
  3. A. Shami, S. Al-Mijalli, P. Pongchaikul, A. Al-Barrag, S. AbduRahim, "The prevalence of the culturable human skin aerobic bacteria in Riyadh, Saudi Arabia", BMC Microbiol, Vol.19, pp. 189, (2019). https://doi.org/10.1186/s12866-019-1569-5
  4. S. Thijs, M. Op De Beeck, B. Beckers, S. Truyens, V. Stevens, J. D. Van Hamme, N. Weyens, J. Vangronsveld, "Comparative Evaluation of Four Bacteria-Specific Primer Pairs for 16S rRNA Gene Surveys", Front Microbiol, Vol.8, pp. 494, (2017).
  5. W. G. Weisburg, S. M. Barns, D. A. Pelletier, D. J. Lane, "16S ribosomal DNA amplification for phylogenetic study", J Bacteriol, Vol.173, pp. 697-703, (1991). https://doi.org/10.1128/JB.173.2.697-703.1991
  6. M. Johnson, I. Zaretskaya, Y. Raytselis, Y. Merezhuk, S. McGinnis, T. L. Madden, "NCBI BLAST: a better web interface", Nucleic Acids Res, Vol.36, pp. W5-9, (2008). https://doi.org/10.1093/nar/gkn201
  7. M. J. Kim, "Differences in Skin Temperature and Perceived Thermal Comfort Based on Age, Sex and Clothing Weight of Participants in a Room at Recommended Room Temperature", Korean J. Community Living Science, Vol.15, pp. 55-64, (2004).
  8. Y. J. Oh, J. Y. Jang, S. K. Lim, M. S. Kwon, J. Lee, N. Kim, M. Y. Shin, H. K. Park, M. J. Seo, H. J. Choi, "Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi", J Microbiol, Vol.55, pp. 933-938, (2017). https://doi.org/10.1007/s12275-017-7386-3
  9. V. Vrbovska, V. Kovarovic, I. Maslanova, A. Indrakova, P. Petras, O. Sedo, P. Svec, L. Fisarova, M. Siborova, K. Mikulasek, I. Sedlacek, J. Doskai, R. Pantucek. "Staphylococcus petrasii diagnostics and its pathogenic potential enhanced by mobile genetic elements", Int J Med Microbiol, Vol.309, pp. Article 151355, (2019).
  10. A. H. Van Craenenbroeck, K. Camps, P. Zachee, K. L. Wu, "Massilia timonae infection presenting as generalized lymphadenopathy in a man returning to Belgium from Nigeria", J Clin Microbiol, Vol.49, pp. 2763-2765, (2011). https://doi.org/10.1128/JCM.00160-11
  11. N. J. Alkhatib, M. H. Younis, A. S. Alobaidi, N. M. Shaath, "An unusual osteomyelitis caused by Moraxella osloensis: A case report", Int J Surg Case Rep, Vol.41, pp. 146-149, (2017). https://doi.org/10.1016/j.ijscr.2017.10.022
  12. Y. Maruyama, T. Shigemura, K. Aoyama, N. Nagano, Y. Nakazawa, "Bacteremia due to Moraxella osloensis: a case report and literature review", Braz J Infect Dis, Vol.22, pp. 60-62, (2018).
  13. A. Yamada, K. Kasahara, Y. Ogawa, K. Samejima, M. Eriguchi, H. Yano, K. Mikasa, K. Tsuruya, "Peritonitis due to Moraxella osloensis: A case report and literature review", J Infect Chemother, Vol.25, pp. 1050-1052, (2019). https://doi.org/10.1016/j.jiac.2019.05.018
  14. I. A. Myles, N. J. Earland, E. D. Anderson, I. N. Moore, M. D. Kieh, K. W. Williams, A. Saleem, N. M. Fontecilla, P. A. Welch, D. A. Darnell, L. A. Barnhart, A. A. Sun, G. Uzel, S. K. Datta, "First-in-human topical microbiome transplantation with Roseomonas mucosa for atopic dermatitis", JCI Insight, Vol.3, pp. e120608, (2018). https://doi.org/10.1172/jci.insight.120608
  15. N. Beucler, M. Meyer, A. Choucha, P. Seng, H. Dufour, "Peritonitis caused by Roseomonas mucosa after ventriculo-peritoneal shunt revision: a case report", Acta Neurochir (Wien), Vol.162, pp. 2459-2462, (2020). https://doi.org/10.1007/s00701-020-04449-9
  16. M. Bhende, A. Karpe, S. Arunachalam, K. L. Therese, J. Biswas, "Endogenous endophthalmitis due to Roseomonas mucosa presenting as a subretinal abscess", J Ophthalmic Inflamm Infect, Vol.7, pp. 5, (2017). https://doi.org/10.1186/s12348-017-0123-6
  17. K. Kimura, H. Hagiya, I. Nishi, H. Yoshida, K. Tomono, "Roseomonas mucosa bacteremia in a neutropenic child: A case report and literature review", IDCases, Vol.14, pp. e00469, (2018). https://doi.org/10.1016/j.idcr.2018.e00469
  18. S. Shao, X. Guo, P. Guo, Y. Cui, Y. Chen, "Roseomonas mucosa infective endocarditis in patient with systemic lupus erythematosus: case report and review of literature", BMC Infect Dis, Vol.19, pp. 140, (2019). https://doi.org/10.1186/s12879-019-3774-0
  19. I. A. Myles, I. N. Moore, C. R. Castillo, S. K. Datta, "Differing Virulence of Healthy Skin Commensals in Mouse Models of Infection", Front Cell Infect Microbio, Vol.8, pp. 451, (2018). https://doi.org/10.3389/fcimb.2018.00451
  20. G. Ianiro, G. Rizzatti, M. Plomer, L. Lopetuso, F. Scaldaferri, F. Franceschi, G. Cammarota, A. Gasbarrini, "Bacillus clausii for the Treatment of Acute Diarrhea in Children: A Systematic Review and Meta-Analysis of Randomized Controlled Trials", Nutrients, Vol.10, No.8, pp. 1074, (2018). https://doi.org/10.3390/nu10081074
  21. S. Joshi, S. Udani, S. Sen, S. Kirolikar, A. Shetty, "Bacillus Clausii Septicemia in a Pediatric Patient After Treatment With Probiotics", Pediatr Infect Dis J, Vol.38, pp. e228-e230, (2019). https://doi.org/10.1097/INF.0000000000002350
  22. Y. E. Chen, M. A. Fischbach, Y. Belkaid, "Skin microbiota-host interactions", Nature, Vol.553, pp. 427-436, (2018). https://doi.org/10.1038/nature25177