DOI QR코드

DOI QR Code

Effect of Pre-Treatment Methods on the Measurement of Probiotic Cell Count

전처리 방법이 유산균수 측정에 미치는 영향

  • Kang, Ju-Yeong (Major in Department of Food Science and Technology, Sunchon National University) ;
  • Lee, Seo-Hyun (Major in Department of Food Science and Technology, Sunchon National University) ;
  • Park, Eun-Ji (Major in Department of Food Science and Technology, Sunchon National University) ;
  • Kim, Jung-Beom (Major in Department of Food Science and Technology, Sunchon National University)
  • 강주영 (순천대학교 식품공학과 대학원) ;
  • 이서현 (순천대학교 식품공학과 대학원) ;
  • 박은지 (순천대학교 식품공학과 대학원) ;
  • 김중범 (순천대학교 식품공학과)
  • Received : 2019.08.08
  • Accepted : 2019.09.16
  • Published : 2019.10.31

Abstract

The purpose of this study was to analyze the effects of the pre-treatment method on the measurement of probiotic cell counts. The probiotic cell count was not significantly different in the pre-treatment method such as experimenters, diluted solution, medium, and homogenization duration. The mean value of probiotic cell count with capsule was $2.2{\times}10^{10}{\pm}9.5{\times}10^9CFU/g$. This probiotic cell count was converted into $2.8{\times}10^{10}{\pm}1.2{\times}10^{10}CFU/g$ based on the net weight. The mean value of probiotic cell count without capsules was $4.3{\times}10^{10}{\pm}1.8{\times}10^{10}CFU/g$. As a result of this comparison, probiotic cell count showed significant difference with and without capsules. Thus, it is suggested that the probiotic cell count is measured by removing the capsule in capsule probiotics.

Keywords

References

  1. Ahn YT. 2014. Development of probiotic dairy products using lactic acid bacteria isolated from human intestine. Food Sci Ind 47:45-54
  2. Ann YG, Jang BC, Park SJ. 2013. Biological activity and improvement effect on irritable bowel syndrome of wax gourd extract and probiotic lactic acid bacteria. Korean J Food Nutr 26:137-145 https://doi.org/10.9799/ksfan.2013.26.1.137
  3. Baek YJ. 1991. Fermented milk and lactic acid bacteria. Microorg Ind 17:60-67
  4. Kim DS, Park ME, Yoo SY. 2013. Stabilization of Lactobacillus with double matrix capsulation. J Korean Oil Chem Soc 30:656-663 https://doi.org/10.12925/jkocs.2013.30.4.656
  5. Kim SY, Kim KH, Youn SY, Yoon SS. 2006. Isolation and identification of the antilisterial Bifidobacterium isolates from the infants fecal samples. J Korean Dairy Technol Sci 24:19-28
  6. Lee JC. 1991. Recent trends in the use of lactic acid bacteria. Microorg Ind 17:36-40
  7. Lilly DM, Stillwell RH. 1965. Probiotics: Growth-promoting factors produced by microorganisms. Science 147:747-748 https://doi.org/10.1126/science.147.3659.747
  8. Ljungh A, Wadstrom T. 2009. Lactobacillus Molecular Biology: From Genomics to Probiotics. 7th ed. pp.1-205. Caister Academic
  9. Metchnikoff E, Yoo S. 2004. The Prolongation of Life: Optimistic Studies. pp.1-266. Springer
  10. Ministry of Food and Drug Safety. 2016. Status of health functional food side effects estimation case report (April 2016). Available from https://www.foodsafetykorea.go.kr/portal/board/boardDetail.do?menu_no=2873&bbs_no=bbs109&ntctxt_no=1056807&menu_grp=MENU_NEW05 [cited 1 March 2019]
  11. Ministry of Food and Drug Safety. 2018a. Production Performance Statistics of Health Functional Foods in 2017. Available from http://www.mfds.go.kr/brd/m_374/view.do?seq=30195&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page=1 [cited 1 March 2019]
  12. Ministry of Food and Drug Safety. 2018b. Notice of Standards of Health Functional Foods (No. 2018-67, 2018.09.05.). Available from https://www.mfds.go.kr/brd/m_211/view.do?seq=14263 [cited 1 March 2019]
  13. National Institute of Food and Drug Safety Evaluation. 2016. Guidelines for Standard Procedures for Preparing Test Methods, Including Food. Available from http://www.nifds.Go.kr/brd/m_15/view.do?seq=8215 [cited 1 March 2019]
  14. Park JS. 2014. The status of fermented milk market and production direction of lactic acid bacteria. J East Asian Soc Diet Life 11:83-109
  15. Wasilewski A, Zielinska M, Storr M, Fichna J. 2015. Beneficial effects of probiotics, prebiotics, synbiotics, and psychobiotics in inflammatory bowel disease. Inflamm Bowel Dis 217:1674-1682
  16. Yoon JA, Shin KO. 2017. Studies on the function of lactic acid bacteria and related yeasts in probiotics: A review. Korean J Food Nutr 30:395-404 https://doi.org/10.9799/ksfan.2017.30.3.395