한국융합학회논문지 (Journal of the Korea Convergence Society)

한국융합학회 (Korea Convergence Society)
권호 표지
DOI QR코드

DOI QR Code

교내 실습실의 유니트체어의 수관관리에 관한 융합연구

A Convergence Study on Waterline Management of Unit Chair in Dental Hygiene Laboratory

  • 최정옥 (영산대학교 치위생학과)
  • Choi, Jung-OK (Department of Dental Hygiene, Youngsan University)
  • 투고 : 2019.09.17
  • 심사 : 2019.10.20
  • 발행 : 2019.10.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구의 목적은 교내 실습실의 유니트체어 수관의 바이오필름을 채취하여 미생물을 분석하고, 기존적인 물빼기방법과 소독제를 사용하는 방법을 적용하여 미생물 수의 변화를 비교하고자 실시하였다. 교내의 수관으로부터 샘플을 채취한 후 R2A배지를 이용하여 배양하였다. 미생물 동정을 거쳐 분리된 미생물의 균종을 분석하였다. 물빼기에 대한 미생물 감소를 확인하기 위해 30초, 60초, 120초 간격으로 샘플을 채취하였고, 소독제의 효과를 확인하기 위해 소독 후 샘플을 채취하였다. 이렇게 수거된 샘플을 통해 얻은 미생물의 정량적 비교는 SPSS 프로그램을 이용하였다. 결과에서 동정된 균주는 총 8개로 나타났으며 대부분 그람음성인 Sphingomonas 계열로 확인되었다. 60초의 물빼기를 통해 미생물의 수가 급감하는 것을 확인하였고, 소독 즉시 미생물은 검출되지 않았다. 본 연구에서 제시된 방법과 결과를 바탕으로 소홀하게 관리될 수 있는 유니트체어의 수관을 관리함으로써 교차 감염을 예방하고 체계적인 관리가 가능할 것이다.

The purpose of this study is to gather biofilm of unit chair waterline in the laboratory, to analyze microorganisms, to apply the existing draw-off method and the method of using disinfectant, and to compare the change of the number of microorganisms. The water was provided by the waterline of the unitchair, and the gathered samples were cultured with the use of R2A agar plate. Bacterial species separated through the identification of microorganisms were analyzed. To identify the decrease of microorganism for draw-off, samples were gathered in the intervals of 30 seconds, 60 seconds and 120 seconds, and to identify the effect of disinfectant, samples after disinfection were gathered. The quantitative comparison of microorganisms through the gathered samples was done by SPSS program. The number of identified bacteria are 8 species, most of which are gram-negative bacterium, and Sphingomonas type. The rapid decrease of the number of microorganism through draw-off for 60 seconds was confirmed, and microorganisms after disinfection weren't detected right away. Based on the method and result of this study, the water pipe of unit chair, which can be neglected easily, can be managed, so cross infection can be prevented, and systemic management can be possible.

키워드

참고문헌

  1. G. H. Hwang. (2012). Development of Control System with Android Operation System for Dentistry Integrated Device. Journal of the Korea Institute of Information and Communication Engineering, 16(3), 635-642. https://doi.org/10.6109/jkiice.2012.16.3.635
  2. H. Y. Yoon & S. Y. Lee. (2015). Bacterial contamination of dental unit water systems in a student clinical simulation laboratory of college of dentistry. J Dent Hyg Sci, 15(2), 232-237. https://doi.org/10.17135/jdhs.2015.15.2.232
  3. K. O. Yun. (2013). Actual status of infection control by the dental hygienist in Korea. J Korean Soc Dent Hyg, 13(3), 369-376. DOI : 10.13065/jksdh.2013.13.3.369.
  4. H. J. Kang. (2018). Convergence Studies on Knowledge, Attitude, and Health Belief Related to Hepatitis B Vaccination and Its Vaccination Rates of the College Students of Dental Hygienics and Dental Laboratory Technology Majors. Journal of the Korea Convergence Society, 9(11), 107-115. https://doi.org/10.15207/JKCS.2018.9.11.107
  5. S. S. Lee, D. A. Kim, S. Y. Song, M. Y. Kim & H. N. Shim. (2016). Awareness and practice of dental unit waterline management in dental hygienist. Journal of Korean Society of Dental Hygiene, 16(4), 507-516. DOI : 10.13065/jksdh.2016.16.04.507
  6. J. E. Moon & M. O. Song. (2017). A Convergence Study about the Performance of Healthcare-Asociated Infection Control Guidelines of Hospital Nurses-based on the Theory of Planed Behavior. Journal of the Korea Convergence Society, 8(5), 117-125, https://doi.org/10.15207/JKCS.2017.8.5.117
  7. J. H. Lee. (2013). A survey of cognition on infection control of the clients in dental hospital. J Korean Soc Dent Hyg. 13(2), 249-260 https://doi.org/10.13065/jksdh.2013.13.2.249
  8. Centers for Disease Control. (1986). Recommended infection-control practices for dentistry. Centers for Disease Control. 35(15), 237-42.
  9. V. A. Merchant. (1991). Herpesviruses and other microorganisms of concern in dentistry. Dent Clin North Am, 35(2), 283-298.
  10. R. S. Klein, K. Freeman, P. E. Taylor & C. E. Stevens. (1991). Occupational risk for hepatitis C virus infection among New York City dentists. Lancet, 338, 8782- 8783.
  11. F. F. Reinthaler, F. Mascher & D. Stunzner. (1998). Serological examinations for antibodies against Legionella species in dental personnel. J Dent Res, 67(6), 942-93. DOI : 10.1177/00220345880670061001.
  12. K. J. Davies, A. M. Herbert, D. Westmoreland & J. Bagg. (1994). Seroepidemiological study of respiratory virus infections among dental surgeons. Br Dent J, 176(7), 262-265. https://doi.org/10.1038/sj.bdj.4808430
  13. D. H. Kang, H. K. Bae & H. S. Kim. (2011). Isolation and Antibacterial Activity of Actinomycetes Producing Growth Inhibition Compounds Against Multi-antibiotic Resistant Pseudomonas Aeruginosa. Korean Society for Biotechnology and Bioengineering Journal 26(1), 19-26.
  14. H. Y. Yoon & S. Y. Lee. (2018). Adhesion and biofilm formation ability of bacteria isolated from dental unit waterlines. J Dent Hyg Sci, 18(2), 69-75. https://doi.org/10.17135/jdhs.2018.18.2.69
  15. J. Szymanska, J. Sitkowska & J. Dutkiewicz. (2008). Microbial contamination of dental unit waterlines. Annals of Agricultural and Environmental Medicine, 15, 173-179.
  16. B. G. Shearer. (1996). Biofilm and the dental office. J Am Dent Assoc, 127(2), 181-189. https://doi.org/10.14219/jada.archive.1996.0166
  17. J. F. Williams, N. Andrews & J. A. Molinari. (1996). Microbial contamination of dental unit waterlines: origins and characteristics. Compendium, 17(6), 538-542.
  18. C. L. Pankhurst & W. A. Coulter. (2007). Do contaminated dental unit waterlines pose a risk of infection. Journal of Dentistry. 35(9), 712-720. https://doi.org/10.1016/j.jdent.2007.06.002
  19. J. Szymanka. (2007). Bacterial contamination of water in dental unit reservoirs. Ann Agric Environ Med, 14(1), 137-40.
  20. S. H. Lee, J. H. Park & K. J. Sa. (2018). Quantitative and qualitative analysis of microorganisms in dental unit water. J Korean Soc Dent Hyg, 18(4), 433-440.
  21. S. T. Kelley, U. Theisen, L. T. Angenent, A. S. Amand & N. R. Pace. (2004). Molecular analysis of shower curtain biofilm microbes. Appl Environ Microbiol, 70(7), 4187-4192. DOI : 10.1128/AEM.70.7.4187-4192.2004].
  22. B. G. Shearer. (1996). Biofilm and the dental office. J Am Dent Assoc, 127(2), 181-189. https://doi.org/10.14219/jada.archive.1996.0166
  23. Centers for Disease Control and Prevention. (2003). Guidelines for infection control in dental health-care settings -2003. Atlanta: Centers for Disease Control and Prevention. 1-76.
  24. S. Ghosh & S. K. Mallick. (2012). Microbial biofilm: contamination in dental chair unit. Indian Medical Gazette, 2(10), 383-387.
  25. C. M. Cobb, C. R Martel, S. A. McKnight, C. Pasley-Mowry, B. L Ferguson & K. Williams. (2002). How does time-dependent dental unit waterline flushing affect planktonic bacteria levels?. J Dent Educ, 66(4), 549-555. https://doi.org/10.1002/j.0022-0337.2002.66.4.tb03534.x