Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Beneficial effect of collagen hydrolysate containing collagen tripeptides on ultraviolet B-induced skin photoaging

콜라겐 트리펩타이드를 함유한 콜라겐 가수분해물의 피부 광노화 예방 효과

  • 김애향 (아미코젠(주) 바이오텍 R&D 센터) ;
  • 하민우 (아미코젠(주) 바이오텍 R&D 센터) ;
  • 김준일 (아미코젠(주) 바이오텍 R&D 센터) ;
  • 박철 (아미코젠(주) 바이오텍 R&D 센터) ;
  • 신용철 (아미코젠(주) 바이오텍 R&D 센터) ;
  • 신대근 (아미코젠(주) 바이오텍 R&D 센터)
  • Received : 2019.06.05
  • Accepted : 2019.08.22
  • Published : 2019.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate anti-photoaging effects of collagen hydrolysate containing collagen tripeptides (CTP) in both HaCaT cells and SKH-1 hairless mice. CTP treatment was nontoxic to HaCaT cells and improved expression of biomarkers associated with aging of skin, such as, collagen 1A, metalloproteinase (MMP)-1, and MMP-13 after subjecting mice to ultraviolet B (UVB) irradiation. In animal studies, the depth and width of wrinkles in the skin of mice were determined upon subjecting them to UVB irradiation. However, positive effects on wrinkles on the skin of mice were seen following CTP supplementation. Collagen content and density of mouse skin were restored following CTP supplementation for 14 weeks after UVB irradiation. These results were based on the effects of CTP on protein levels of collagen 1A, MMP-1, and MMP-13. Therefore, CTP might have positive effects on the number, depth, and width of wrinkles caused by UVB irradiation in SKH-1 hairless mice.

본 연구는 식용 어류로부터 획득한 콜라겐 중 Gly-Pro-Hyp를 주요 지표로 하는 고함량 콜라겐 트리펩타이드에 대한 피부의 광노화 억제 효과를 확인하고자 HaCaT 세포와 SKH-1 hairless 마우스를 활용한 전임상 실험을 실시하였다. HaCaT 세포는 UVB 조사 이후 Collagen 1A와 MMP-1 그리고 -13의 변화를 관찰하였으며 마우스 실험은 총 50마리의 마우스를 5군으로 구분한 다음 NOR을 제외한 모든 마우스에 UVB를 조사하고 양성대조군인 retinoic acid 혹은 실험물질인 CTP (17, 34 mg/kg)를 섭취시키며 실험하였다. CTP는 HaCaT 세포의 Collagen 1A 증가 및 MMP-1와 -13 감소에 긍정적인 영향을 미쳤을 뿐만 아니라 UVB 조사를 통해 증가된 실험동물의 피부 주름 수와 깊이 그리고 넓이를 현저히 개선시키는 효과를 나타내었다. 특히, CTP의 섭취는 UVB 조사에 따라 파괴된 피부조직 내 콜라겐의 양과 밀도를 정상적으로 회복시켰을 뿐만 아니라 Collagen 1A의 발현 증가 및 MMP1과 -13의 발현 감소를 유도함으로써 UVB 조사에 의한 주름 생성을 감소시킨 효과를 보여주었다. 따라서, 본 실험은 CTP 섭취가 콜라겐 붕괴에 관여하는 MMPs 단백질 발현을 감소시키고 이와는 반대로 Collagen 1A의 발현은 증가시킴을 확인함으로써 피부 외관의 주름 수와 넓이 혹은 깊이를 개선시키는 것에 긍정적인 영향을 미치는 것으로 판단할 수 있다.

Keywords

References

  1. Afaq F, Zaid MA, Khan N, Dreher M, Mukhtar H. Protective effect of pomegranate derived products on UVB-mediated damage in human reconstituted skin. Exp. Dermatol. 18: 553-561 (2009) https://doi.org/10.1111/j.1600-0625.2008.00829.x
  2. Borumand M, Sibilla S. Daily consumption of the collagen supplement pure gold collagen reduces visible signs of aging. Clin. Interv. Aging. 9: 1747-1758 (2014) https://doi.org/10.2147/CIA.S65939
  3. Chai HJ, Li JH, Huang HN, Li TL, Chan YL, Shiau CY, Wu CJ. Effects of sizes and conformations of fish-scale collagen peptides on facial skin qualities and transdermal penetration efficiency. J. Biomed. Biotechnol. Article ID 757301 (2010)
  4. Cho JM, Lee YH, Baek R-M, Lee SW. Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. J. Plast. Reconstr. Aes. 64: e31-e39 (2011) https://doi.org/10.1016/j.bjps.2010.08.014
  5. Dhital B, Durlik P, Rathod P, Gul-E-Noor F, Wang Z, Sun C, Chang EJ, Itin B, Boutis GS. Ultraviolet radiation reduces desmosine cross-links in elastin. Biochem. Biophys. Rep. 10: 172-177 (2017)
  6. Jeon HY, Kim JK, Seo DB, Lee SJ. Effects of chlorophyll a on UVB-induced cellular responses and type I pN collagen synthesis in vitro. Korean J. Food Sci. Technol. 41: 700-705 (2009)
  7. Jung K, Seifert M, Herrling TH, Fuchs J. UV-generated free radicals (FR) in skin: Their prevention by sunscreens and their induction by self-tanning agents. Spectrochim. Acta. A. 69: 1423-1428 (2008) https://doi.org/10.1016/j.saa.2007.09.029
  8. Khan BA, Akhtar N, Rasul A, Khan H, Murtaza G, Ali A, Khan KA, Zaman S, Jameel A, Waseem K, Mahmood T. Human skin, aging and antioxidants. J. Med Plants Res. 6: 1-6 (2012) https://doi.org/10.3923/rjmp.2012.1.1
  9. Kim DU, Chung HC, Choi J, Sakai Y, Lee BY. Oral intake of lowmolecular-weight collagen peptide improves hydration, elasticity and wrinkling in human skin: A randomized, double-blind, placebo-controlled study. Nutrients. 10: 826 (2018a) https://doi.org/10.3390/nu10070826
  10. Kim AH, Kim YS, Piao Z, Shin YC, Ha MW. Cutaneous hydration effect of collagen hydrolysate containing collagen tripeptides. Korean J. Food Sci. Technol. 50: 420-429 (2018b) https://doi.org/10.9721/KJFST.2018.50.4.420
  11. Kim JK, Lee JH, Yang MS, Seo DB, Lee SJ. Beneficial effect of collagen peptide supplement on anti-aging against photodamage. Korean J. Food Sci. Technol. 41: 441-445 (2009)
  12. Kim MS, Oh GH, Kim MJ, Hwang JK. Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells. Photochem. Photobiol. 89: 911-918 (2013) https://doi.org/10.1111/php.12061
  13. Korac RR, Khambholja KM. Potential of herbs in skin protection from ultraviolet radiation. Pharmacogn. Rev. 5: 164-173 (2011) https://doi.org/10.4103/0973-7847.91114
  14. Lee JY. Lee SY. Inhibitory efficacy of Smilaxchina L. on pro-collagen type-1 activity and MMP-1 gene expression in fibroblasts (CCD-986sk). J. Life Sci. 23: 1239-245 (2013) https://doi.org/10.5352/JLS.2013.23.10.1239
  15. Lee JH, Seo JH, Park YH, Kim WG, Lim KM, Lee SJ. The Effect of Hydroxyproline and Pro-Hyp Dipeptide on UV-damaged Skin of Hairless Mice. Korean J. Food Sci. Technol. 40: 436-442 (2008)
  16. Lee MH, Kim HJ, Jung HA, Lee YK. Stimulation of the extracellular matrix production in dermal fibroblast by Areca catechu extract. J. Korea Acad. Industr. Coop. Soc. 14: 1857-1862 (2013) https://doi.org/10.5762/KAIS.2013.14.4.1857
  17. Matts PJ. Solar ultraviolet radiation: definitions and terminology. Dermatol. Clin. 24: 1-8 (2006) https://doi.org/10.1016/j.det.2005.08.004
  18. Matsuda N, Koyama Y-I, Hosaka Y, Ueda H, Watanabe T, Araya T, Irie S, Takehana K. Effects of ingestion of collagen peptide on collagen fibrils and glycosaminoglycans in the dermis. J. Nutr. Sci. Vitaminol. 52: 211-215 (2006) https://doi.org/10.3177/jnsv.52.211
  19. Masaki H. Role of antioxidants in the skin: Anti-aging effects. J. Dermatol. Sci. 58: 85-90 (2010) https://doi.org/10.1016/j.jdermsci.2010.03.003
  20. Misato Y, Yukihiko I, Masayoshi Y, Spyros G, Sachiyuki T, Masa-aki N, Shigeo O, Kohji Y. Oral Ingestion of collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin. J. Agr. Food Chem. 65: 2315-2322 (2017) https://doi.org/10.1021/acs.jafc.6b05679
  21. Mohamed MAA, Jung M, Lee SM, Lee TH, Kim J. Protective effect of Disporum sessile D. Don extract against UVB-induced photoaging via suppressing MMP-1 expression and collagen degradation in human skin cells. J. Photoch. Photobio. B. 133: 73-79 (2014) https://doi.org/10.1016/j.jphotobiol.2014.03.002
  22. Mukherjee PK, Maity N, Nema NK, Sarkar BK. Bioactive compounds from natural resources against skin aging. Phytomedicine. 19: 64-73 (2011) https://doi.org/10.1016/j.phymed.2011.10.003
  23. Postlethwaite AE, Seyer JM, Kang AH. Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagenderived peptides. Proc. Natl. Acad. Sci. 75: 871-875 (1978) https://doi.org/10.1073/pnas.75.2.871
  24. Pyun H-B, Kim M, Park J, Sakai Y, Numata N, Shin J-Y, Shin H-J, Kim D-U, Hwang J-K. Effects of collagen tripeptide supplement on photoaging and epidermal skin barrier in UVB-exposed hairless mice. Prev. Nutr. Food Sci. 17: 245-253 (2012) https://doi.org/10.3746/pnf.2012.17.4.245
  25. Shin H-J, Park JI, Kwon C-J, Kim HY, Inoue N, Seiko K, Hwang JS. The effect of collagen peptide intake on UVB-induced skin damage in hairless mice. J. Korea Acad. Industr. Coop. Soc. 17: 611-621 (2016)
  26. Sontakke SB, Jung J-H, Piao Z, Chung HJ. Orally available collagen tripeptide: Enzymatic stability, intestinal permeability and absorption of Gly-Pro-Hyp and Pro-Hyp. J. Agric. Food Chem. 64: 7127-7133 (2016) https://doi.org/10.1021/acs.jafc.6b02955
  27. Watson REB, Gibbs NK, Griffiths CEM, Sherratt MJ. Damage to skin extracellular matrix induced by UV exposure. Antioxid. Redox. Sign. 21: 1063-1077 (2014) https://doi.org/10.1089/ars.2013.5653
  28. Yoon Y, Bae S, An S, Choe YB, Ahn KJ, An IS. Effects of ultraviolet radiation on the skin and skin cell signaling pathways. J. Korea Aesthet. Cosmetol. 11: 417-426 (2013)
  29. Zague V, de Freitas V, Da Costa Rosa M, de Castro GA, Jaeger RG, Machado-Santelli GM. Collagen hydrolysate intake increases skin collagen expression and suppresses matrix metalloproteinase 2 activity. J. Med. Food. 14: 618-624 (2011) https://doi.org/10.1089/jmf.2010.0085