Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effects of Streptomyces sp. MBTH32 Metabolites on Sortase A and Sortase A-Mediated Cell Clumping of Staphylococcus aureus to Fibrinogen

  • Chung, Beomkoo (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kwon, Oh-Seok (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Jongheon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Oh, Ki-Bong (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2019.06.13
  • Accepted : 2019.08.28
  • Published : 2019.10.28
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Abstract

Sortase A (SrtA), a type of transpeptidase responsible for anchoring surface proteins to the peptidoglycan cell wall, is important in the virulence of gram-positive bacteria. Three compounds were isolated from marine-derived Streptomyces sp. MBTH32 using various chromatography techniques. The structures of these compounds were determined based on spectroscopic data and comparisons with previously reported data. Among the metabolites tested, lumichrome showed strong inhibitory activity against Staphylococcus aureus SrtA without affecting cell viability. The results of cell clumping activity assessment suggest the potential for using this compound to treat S. aureus infection by inhibiting SrtA activity.

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References

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