Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Impact of Ecklonia stolonifera extract on in vitro ruminal fermentation characteristics, methanogenesis, and microbial populations

  • Lee, Shin Ja (Institute of Agriculture and Life Science and University-Centered Labs, Gyeongsang National University) ;
  • Jeong, Jin Suk (Division of Applied Life Science (BK21Plus) and Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Shin, Nyeon Hak (Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute) ;
  • Lee, Su Kyoung (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hyun Sang (Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Eom, Jun Sik (Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Lee, Sung Sill (Institute of Agriculture and Life Science and University-Centered Labs, Gyeongsang National University)
  • Received : 2019.02.01
  • Accepted : 2019.05.16
  • Published : 2019.12.01
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Abstract

Objective: This study was conducted to evaluate the effects of Ecklonia stolonifera (E. stolonifera) extract addition on in vitro ruminal fermentation characteristics, methanogenesis and microbial populations. Methods: One cannulated Holstein cow ($450{\pm}30kg$) consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (09:00 and 17:00) at 2% of body weight with free access to water and mineral block were used as rumen fluid donors. In vitro fermentation experiment, with timothy hay as substrate, was conducted for up to 72 h, with E. stolonifera extract added to achieve final concentration 1%, 3%, and 5% on timothy hay basis. Results: Administration of E. stolonifera extract to a ruminant fluid-artificial saliva mixture in vitro increased the total gas production. Unexpectedly, E. stolonifera extracts appeared to increase both methane emissions and hydrogen production, which is contrasts with previous observations with brown algae extracts used under in vitro fermentation conditions. Interestingly, real-time polymerase chain reaction indicated that as compared with the untreated control the ciliate-associated methanogen and Fibrobacter succinogenes populations decreased, whereas the Ruminococcus flavefaciens population increased as a result of E. stolonifera extract supplementation. Conclusion: E. stolonifera showed no detrimental effect on rumen fermentation characteristics and microbial population. Through these results E. stolonifera has potential as a viable feed supplement to ruminants.

Keywords

References

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