The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur

  • Shin, Yong Ho (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Ko, Eun Ju (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Kim, Su Jeong (Postech Biotech Center, Pohang University of Science and Technology) ;
  • Hyun, He Nam (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Jeun, Yong Chull (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
  • 투고 : 2019.04.17
  • 심사 : 2019.06.19
  • 발행 : 2019.10.01
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초록

Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

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참고문헌

  1. Agostini, J. P., Bushong, P. M., Bhatia, A. and Timmer, L. W. 2003. Influence of environmental factors on severity of citrus scab and melanose. Plant Dis. 87:1102-1106. https://doi.org/10.1094/PDIS.2003.87.9.1102
  2. Bushong, P. M. and Timmer, L. W. 2000. Evaluation of postinfection control of citrus scab and melanose with benomyl, fenbuconazole, and azoxystrobin. Plant Dis. 84:1246-1249. https://doi.org/10.1094/PDIS.2000.84.11.1246
  3. Choudhury, S. R., Ghosh, M., Mandal, A., Chakravorty, D., Pal, M., Pradhan, S. and Goswami, A. 2011. Surface-modified sulfur nanoparticles: an effective antifungal agent against Aspergillus niger and Fusarium oxysporum. Appl. Microbiol. Biotechnol. 90:733-743. https://doi.org/10.1007/s00253-011-3142-5
  4. Fu, L.-H., Hu, K.-D., Hu, L.-Y., Li, Y.-H., Hu, L.-B., Yan, H., Liu, Y.-S. and Zhang, H. 2014. An antifungal role of hydrogen sulfide on the postharvest pathogens Aspergillus niger and Penicillium italicum. PLoS ONE 9:e104206. https://doi.org/10.1371/journal.pone.0104206
  5. Gabler, F. M., Mercier, J., Jimenez, J. I. and Smilanick, J. L. 2010. Integration of continuous biofumigation with Muscodor albus with pre-cooling fumigation with ozone or sulfur dioxide to control postharvest gray mold of table grapes. Postharvest Biol. Technol. 55:78-84. https://doi.org/10.1016/j.postharvbio.2009.07.012
  6. Gogoi, R., Singh, P. K., Kumar, R., Nair, K. K., Alam, I., Srivastava, C., Yadav, S., Gopal, M., Choudhury, S. R. and Goswami, A. 2013. Suitability of nano-sulphur for biorational management of powdery mildew of okra (Abelmoschus esculentus Moench) caused by Erysiphe cichoracearum. J. Plant Pathol. Microbiol. 4:171.
  7. Gopal, K., Lakshmi, L. M., Sarada, G., Nagalakshmi, T., Sankar, T. G., Gopi, V. and Ramana, K. T. V. 2014. Citrus melanose (Diaporthe citri Wolf): a review. Int. J. Curr. Microbiol. Appl. Sci. 3:113-124.
  8. Han, S. G. 2012. The productivity and the evaluation of environmental impact with characteristics environmentally friendly agricultural materials. Ph.D. thesis. Chungnam National University, Daejeon, Korea. 104 pp.
  9. Hyun, J.-W., Kim, D.-H., Kim, K.-S., Lee, S.-C., Ko, S.-W. and Lim, H.-C. 2004. Diseases and the symptoms recently occurred on 'Shiranuhi' citrus cultivar in Jeju island. Res. Plant Dis. 10:94-99 (in Korean). https://doi.org/10.5423/RPD.2004.10.2.094
  10. Hyun, J.-W., Ko, S.-W., Kim, D.-H., Han, S.-G., Kim, K.-S., Kwon, H.-M. and Lim, H.-C. 2005. Effective usage of copper fungicides for environment-friendly control of citrus diseases. Res. Plant Dis. 11:115-121. https://doi.org/10.5423/RPD.2005.11.2.115
  11. Hyun, J.-W., Yi, P.-H., Hwang, R.-Y. and Moon, K.-H. 2013. Aspect of incidence of the major citrus diseases recently. Res. Plant Dis. 19:102-107 (in Korean). https://doi.org/10.5423/RPD.2013.19.2.102
  12. Jeong, H. K., Sung, J. H. and Lee, H. J. 2018. Current issue and policy on market condition of internal and external environment-friendly agricultural products in 2018. Vol. 169. Korea Rural Economic Institute, Naju, Korea. 11 pp.
  13. Kang, S.-K. and Ko, B.-H. 2018. A causality analysis of the tangerine market by distribution channel. J. Korea Acad. Ind. Coop. Soc. 19:376-381. https://doi.org/10.5762/KAIS.2018.19.3.376
  14. Kim, S. J., Kim, E. S., Kim, S. H. and Yun, H. K. 2018. Inhibitory effects of environment-friendly materials and defense response signaling chemicals against anthracnose occurrence in Jujube (Zizyphus jujuba Miller). Korean J. Agric. Sci. 45:365-378. https://doi.org/10.7744/KJOAS.20180062
  15. Ko, Y. J., Kang, S. Y. and Jeun, Y. C. 2012. Suppression of citrus melanose on the leaves treated with rhizobacterial strains after inoculation with Diaporthe citri. Res. Plant Dis. 18:331-337 (in Korean). https://doi.org/10.5423/RPD.2012.18.4.331
  16. Kolaei, E. A., Tweddell, R. J. and Avis, T. J. 2012. Antifungal activity of sulfur-containing salts against the development of carrot cavity spot and potato dry rot. Postharvest Biol. Technol. 63:55-59. https://doi.org/10.1016/j.postharvbio.2011.09.006
  17. Kwon, H.-M., Nam, K.-W., Kim, K.-S., Kim, D.-H., Lee, S.-C. and Hyeon, J.-W. 2003. Characterization of the causal fungus of citrus melanose, Diaphorthe citri isolated from blighted twigs of citrus in Jeju. Res. Plant Dis. 9:153-158 (in Korean). https://doi.org/10.5423/RPD.2003.9.3.153
  18. Lee, J. B., Hong, S.-M., Kwon, H.-Y., You, A.-S., Hong, S.-S. and Ihm, Y. 2014a. Safety evaluation of pesticide residue in five fruits by dietary risk index. Korean J. Pestic. Sci. 18:336-341. https://doi.org/10.7585/kjps.2014.18.4.336
  19. Lee, S. W., Lee, S. H., Park, K. H., Jang, I. B., Jin, M. L. and Kim, K. H. 2014b. Effect of irrigation of sulfur solution before sowing on growth and root rot disease of seedling in ginseng nursery. Korean J. Med. Crop Sci. 22:391-397. https://doi.org/10.7783/KJMCS.2014.22.5.391
  20. Lim, J.-S., Mo, H.-S., Lee, E.-H., Park, K.-C. and Chung, C.-M. 2015. Suppressive effects of sulfur-containing compounds on ginseng anthracnose (Colletotrichum gloeosporioides) and proper application concentration. Korean J. Environ. Agric. 34:46-51. https://doi.org/10.5338/KJEA.2015.34.1.03
  21. Miles, A. K., Willingham, S. L. and Cooke, A. W. 2004. Field evaluation of strobilurins and a plant activator for the control of citrus black spot. Australas. Plant Pathol. 33:371-378. https://doi.org/10.1071/AP04025
  22. Mondal, S. N., Vicent, A., Reis, R. F. and Timmer, L. W. 2007. Saprophytic colonization of citrus twigs by Diaporthe citri and factors affecting pycnidial production and conidial survival. Plant Dis. 91:387-392. https://doi.org/10.1094/PDIS-91-4-0387
  23. Nam, K.-W. and Kim, S.-H. 2002. Effect on fruit quality and tree's main disease control by agro-chemical alternatives. Korean J. Org. Agric. 10:67-77.
  24. Nnam, M.-H., Shin, J.-H., Choi, J.-P., Hong, S.-I., Kim, Y.-G. and Kim, H.-T. 2009. Identification of Rhizo-bacterium inhibiting Diaporthe citri causing citrus melanose. Korean J. Pestic. Sci. 13:332-335.
  25. Oh, S.-Y., Nam, K.-W. and Yoon, D.-H. 2014. Effect of environmental-friendly control for white stain symptom on grape. Korean J. Environ. Agric. 33:184-188. https://doi.org/10.5338/KJEA.2014.33.3.184
  26. Park, J. S., Seo, Y. A., Kin, K. R. and Ha, J.-C. 2018. Evaluating the prediction models of leaf wetness duration for citrus orchards in Jeju, South Korea. Korean J. Agric. For. Meterol. 20:262-276.
  27. Reuveni, M., Gur, L. and Farber, A. 2018. Development of improved disease management for powdery mildew on mango trees in Israel. Crop Prot. 110:221-228. https://doi.org/10.1016/j.cropro.2017.07.017
  28. Ryu, Y.-H., Huh, C.-S., Kim, D.-G., Yeon, I.-K., Jo, W.-S. and Ryu, J.-A. 2015. Controlling effect of some environmentally friendly agents on garlic leaf blight in garlic. Korean J. Org. Agric. 23:347-357. https://doi.org/10.11625/KJOA.2015.23.2.347
  29. Shim, C.-K., Kim, M.-J., Kim, Y.-K., Hong, S.-J. and Kim, S.-C. 2014. Reducing phytotoxic by adjusted pH and control effect of loess-sulfur complex as organic farming material against powdery mildew in tomato. Korean J. Pestic. Sci. 18:376-382. https://doi.org/10.7585/kjps.2014.18.4.376
  30. Smilanick, J. L., Hartsell, P. I., Henson, D., Fouse, D. C., Assemi, M. and Harris, C. M. 1990. Inhibitory activity of sulfur dioxide on the germination of spores of Botrytis cinerea. Phytopathology 80:217-220. https://doi.org/10.1094/Phyto-80-217
  31. Smith, D. and Papacek, D. F. 1991. Studies of the predatory mite Amblyseius victoriensis (Acarina: Phytoseiidae) in citrus orchards in south-east Queensland: control of Tegolophus australis and Phyllocoptruta oleivora (Acarina: Eriophyidae), effect of pesticides, alternative host plants and augmentative release. Exp. Appl. Acarol. 12:195-217. https://doi.org/10.1007/BF01193467
  32. Wu, W., Zhang, C., Chen, L., Li, G., Wang, Q. and Shi, J. 2018. Inhibition of hydrogen sulfide and hypotaurine on Monilinia fructicola disease in peach fruit. Acta Hortic. 1194:257-266. https://doi.org/10.17660/actahortic.2018.1194.38
  33. Yi, P.-H., Hyun, J.-W., Hwang, R.-Y. and Kim, K.-S. 2014. Improvement of control efficacy of mancozeb wettable powder against citrus melanose by mixing with paraffin oil. Res. Plant Dis. 20:196-200 (in Korean). https://doi.org/10.5423/RPD.2014.20.3.196