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소 분변에서 분리된 Celluosilyticum lentocellum WCF-2의 유전체 염기서열 분석

Complete genome sequence of Celluosilyticum lentocellum WCF-2 isolated from cow dung

  • 허준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 유재홍 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박인철 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 한병학 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 권순우 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 안재형 (농촌진흥청 국립농업과학원 농업미생물과)
  • Heo, Jun (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • You, Jaehong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Park, InCheol (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Han, Byeong-Hak (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kwon, Soon-Wo (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2019.09.09
  • 심사 : 2019.09.18
  • 발행 : 2019.09.30

초록

사일리지 제조에 사용하기 위한 섬유소 분해균을 탐색하는 중 절대혐기성 세균인 WCF-2 균주를 선발하였다. WCF-2 균주는 16S rRNA 유전자 염기서열 유사도가 가장 높은(98.2%) 표준균주인 Cellulosilyticum lentocellum DSM $5427^T$ 보다 높은 섬유소 분해 활성을 나타내었다. WCF-2 균주의 전체 유전체 염기서열을 분석하고 이를 C. lentocellum DSM $5427^T$와 비교하였을 때 두 균주의 OrthoANI 값은 97.9%로 나타나 WCF-2를 C. lentocellum으로 동정하였다. WCF-2 균주의 유전체 크기는 4,779,774 bp이고 G + C 함량은 34.4%였으며 4,154개의 단백질 암호화 유전자 및 142개의 RNA 암호화 유전자를 보유하고 있었다. 또한 WCF-2 균주는 7개의 cellulase를 보유하고 있었으며 이 중 5개는 C. lentocellum DSM $5427^T$의 cellulase와 낮은 유사도를 나타내었다.

An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM $5427^T$, the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity). We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM $5427^T$. The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM $5427^T$.

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참고문헌

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