Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Ralstonia pickettii Enhance the DDT Biodegradation by Pleurotus eryngii

  • Purnomo, Adi Setyo (Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo) ;
  • Maulianawati, Diana (Department of Aquaculture, Faculty of Fisheries and Marine Science, Borneo Tarakan University) ;
  • Kamei, Ichiro (Department of Forest and Environmental Science, Faculty of Agriculture, University of Miyazaki)
  • Received : 2019.06.14
  • Accepted : 2019.08.28
  • Published : 2019.09.28
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Abstract

DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactant-producing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml ($1ml{\approx}1.44{\times}10^{13}CFU$). After 7 d incubation, about 43% of the initial DDT ($12.5{\mu}M$) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

Keywords

References

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