한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제47권3호
  • /
  • Pages.337-342
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  • 2019
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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재조합 Corynebacterium glutamicum으로부터 헴첼 생산에 미치는 프로모터의 효과

Effect of Promoters on the Heme Production in a Recombinant Corynebacterium glutamicum

  • 양형모 (가톨릭대학교 생명공학과) ;
  • 김필 (가톨릭대학교 생명공학과)
  • Yang, Hyungmo (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Pil (Department of Biotechnology, The Catholic University of Korea)
  • 투고 : 2018.07.02
  • 심사 : 2018.09.15
  • 발행 : 2019.09.28
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초록

We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase ($hemA^+$) under control of a constitutive promoter ($P_{180}$) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the $hemA^+$ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The $hemA^+$ expression by $P_{gapA}$ (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by $P_{H36}$ (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control ($hemA^+$ expression by $P_{180}$ constitutive promoter). The $hemA^+$ expression by a late log-phase activating $P_{sod}$ (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The $hemA^+$ expression led by a heat-shock responding chaperone promoter ($P_{dnaK}$) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter strength and induction phase are discussed based on the results for the heme production at an industrial scale.

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참고문헌

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