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Hormone Analysis during Artificial Estrus Induction in Korean Black Goats

흑염소의 인공수정 발정동기화 처리방법에 따른 호르몬 변화분석

  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Jeon, Dayeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 전다연 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김승창 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2019.07.02
  • Accepted : 2019.08.02
  • Published : 2019.08.31

Abstract

The purpose of the study is to investigate the change in the blood progesterone (P4) and estrogen (E2) levels when applying different estrus induction protocols to Korean black goats, and this was done to gain understanding about their reproduction physiology. For the experiment, we performed three estrus induction protocols that are commonly used in bovine: controlled internal drug release (CIDR) + prostaglandin $F2{\alpha}$ ($PGF2{\alpha}$), $PGF2{\alpha}$ + gonadotropin-releasing hormone (GnRH) + $PGF2{\alpha}$, and CIDR + $PGF2{\alpha}$ + PMSG. The P4 and E2 concentrations showed different patterns until the last treatment of the three protocols. However, similar concentration patterns were shown after the last treatment in all the protocols. In conclusion, we monitored the blood P4 and E2 levels in Korean black goats following three different estrus induction protocols. Our findings may be used in other breeding programs of Korean black goats, such as artificial insemination and embryo transfer.

본 연구의 목적은 한국 재래흑염소 번식생리의 이해를 돕고자 서로 다른 발정동기화법을 적용하였을 때 혈액내 프로게스테론과 에스트로겐의 농도변화를 알아본 것이다. 발정동기화법은 소의 방법을 기본으로 하여 품종의 크기에 따라 호르몬의 용량을 조절하는 것을 가장 많이 사용되고 있으나, 흑염소에 적용하였을 때 발정동기화 후 인공수정 적기의 판단이 정확하지 않아 효율이 매우 낮게 조사된다. 연구에 조사된 발정동기화 방법으로는 소에서 많이 이용되어왔던 CIDR + $PGF2{\alpha}$, $PGF2{\alpha}$ + GnRH + $PGF2{\alpha}$, 그리고 CIDR + $PGF2{\alpha}$ + PMSG 세가지를 적용하였다. 혈액내 프로게스테론과 에스트로겐은 발정동기화 각 처치 후와 최종 처치 후 수일간의 변화를 조사하였다. 혈액내 프로게스테론과 에스트로겐 농도를 조사한 결과, 세가지 발정동기화 방법 마지막 처치 전까지는 모두 다른 변화를 보였으나, 그 후로는 서로 유사한 패턴으로 호르몬이 변화하는 것을 관찰할 수 있었다. 결론적으로 발정동기화를 위한 호르몬 주사가 개체에 서로 다르게 작용하여 발정을 유도하고 최종 처치 후로는 유사한 발정이 유도되어 유사한 호르몬 상태를 보인 것으로 생각된다. 본 연구의 결과를 한국 재래흑염소 번식에서 인공수정이나 수정란 이식과 같은 기술에 접목한다면 효율적인 개량유도에 기여할 것으로 판단된다.

Keywords

References

  1. L. L. Mamuad, S. H. Kim, S. S. Lee, K. K. Cho, C. O. Jeon, S. S. Lee. Characterization, metabolites and gas formation of fumarate reducing bacteria isolated from Korean native goat (Capra hircus coreanae). Journal of Microbiology (Seoul, Korea), no. 6, vol. 50, pp. 925-931, 2012. DOI: https://doi.org/10.1007/s12275-012-2497-3
  2. B. Y. Jung, M. G. Seo, S. H. Lee, J. W. Byun, J. K. Oem, D. Kwak. Molecular and serologic detection of Coxiella burnetii in native Korean goats (Capra hircus coreanae ). Veterinary Microbiology, no. 1-2, vol. 173, pp. 152-155, 2014. DOI: https://doi.org/10.1016/j.vetmic.2014.06.029
  3. S. W. Kim, S. B. Park, M. J. Kim, D. H. Kim, D. G. Yim. Effects of different levels of concentrate in the diet on physicochemical traits of Korean native black goat meats. Korean Journal for Food Science of Animal Resources, no. 4, vol. 34, pp. 457-463, 2014. DOI: https://doi.org/10.5851/kosfa.2014.34.4.457
  4. A. M. Reza, S. Shiwani, N. K. Singh, J. D. Lohakare, S. J. Lee, D. K. Jeong, et al. Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage. In Vitro Cellular and Developmental Biology. Animal, no. 3, vol. 50, pp. 194-206, 2014. DOI: https://doi.org/10.1007/s11626-013-9690-5
  5. S. Y. Jang, E. K. Kim, J. H. Park, M. R. Oh, Y. J. Tang, Y. L. Ding, et al. Effects of physically effective neutral detergent fiber content on dry matter intake, digestibility, and chewing activity in Korean native goats (Capra hircus coreanae ) fed with total mixed ration. Asian-Australasian Journal of Animal Sciences, no. 10, vol. 30, pp. 1405-1409. 2017. DOI: https://doi.org/10.5713/ajas.16.0868
  6. A. Bakhsh, I. Ismail, Y. H. Hwang, J. G. Lee, S. T. Joo. Comparison of blood loss and meat quality characteristics in Korean black goat subjected to head-only electrical stunning or without stunning. Korean Journal of Food Science of Animal Resources, no. 6, vol. 38, pp. 1286-1293, 2018. DOI: https://doi.org/10.5851/kosfa.2018.e64
  7. Y. H. Hwang, A. Bakhsh, I. Ismail, J. G. Lee, S. T. Joo. Effects of Intensive Alfalfa Feeding on Meat Quality and Fatty Acid Profile of Korean Native Black Goats. Korean J Food Sci Anim Resour. 38(5):1092-1100. 2018. DOI: https://doi.org/10.5851/kosfa.2018.e42
  8. A. Bakhsh, I. Ismail, Y. H. Hwang, J. G. Lee, S. T. Joo. Comparison of Blood Loss and Meat Quality Characteristics in Korean Black Goat Subjected to Head-Only Electrical Stunning or without Stunning. Korean J Food Sci Anim Resour. 38(6):1286-1293. 2018. DOI: https://doi.org/10.5851/kosfa.2018.e64
  9. N. T. Kusina, Tarwirei, H. Hamudikuwanda, G. Agumba, J. Mukwena. A comparison of the effects of progesterone sponges and ear implants, PGF2alpha, and their combination on efficacy of estrus synchronization and fertility of Mashona goat does. Theriogenology, no. 8, vol. 53, pp. 1567-1580, 2000. DOI: https://doi.org/10.1016/s0093-691x(00)00298-3
  10. B. Leboeuf, B. Restall, S. Salamon. Production and storage of goat semen for artificial insemination. Animal Reproduction Science, no. 1-3, vol. 62, pp. 113-141, 2000. DOI: https://doi.org/10.1016/s0378-4320(00)00156-1
  11. L. Shi, Y. Ren, H. Zhou, G. Hou, W. Xun, W. Yue, et al. Effect of rapid freezing-thawing techniques on the sperm parameters and ultrastructure of Chinese Taihang black goat spermatozoa. Micron (Oxford, England), vol. 57, pp. 6-12, 2014. DOI: https://doi.org/10.1016/j.micron.2013.09.004
  12. H. Baldassarre, C. N. Karatzas. Advanced assisted reproduction technologies (ART) in goats. Animal Reproduction Science, vol. 82-83, pp. 255-266, 2004. DOI: https://doi.org/10.1016/j.anireprosci.2004.04.027
  13. Y. Zhao, J. Zhang, H. Wei, X. Sun, B. Mu, M. Yu, et al. Efficiency of methods applied for goat estrous synchronization in subtropical monsoonal climate zone of Southwest China. Tropical Animal Health and Production, no. 6, vol. 42, 1257-1262, 2010. DOI: https://doi.org/10.1007/s11250-010-9558-6
  14. P. Minoia, G. M. Lacalandra, A. Zarrilli. [Estrus induction in the goat after pulsatile administration of LHFSHRH]. Bollettino della Societa Italiana Biologia Sperimentale, no. 11, vol. 59, pp. 1625-1630, 1983.
  15. N. T. Kusina, T. Chinuwo, H. Hamudikuwanda, L. R. Ndlovu, S. Muzanenhamo. Effect of different dietary energy level intakes on efficiency of estrus synchronization and fertility in Mashona goat does. Small Ruminant Research, no. 3, vol. 39, pp. 283-288, 2001. DOI: https://doi.org/10.1016/s0921-4488(00)00192-9
  16. J. R. Pursley, M. O. Mee, M. C. Wiltbank. Synchronization of ovulation in dairy cows using PGF2alpha and GnRH. Theriogenology, no. 7, vol. 44, pp. 915-923, 1995. DOI: https://doi.org/10.1016/0093-691x(95)00279-h
  17. W. Holtz, B. Sohnrey, M. Gerland, M. A. Driancourt. Ovsynch synchronization and fixed-time insemination in goats. Theriogenology, no. 7, vol. 69, pp. 785-792, 2008. DOI: https://doi.org/10.1016/j.theriogenology.2007.10.004
  18. H. Miura, S. Kotani, M. Kohiruimaki, H. Ohtsuka, M. Kikuchi, Y. Ohnami. Relationships between the conception rate of estrus synchronization using estradiol benzoate and CIDR (progesterone) and other parameters in holstein lactating dairy cows. The Journal of Reproduction and Development, no. 3, vol. 54, pp. 214-216, 2008. DOI: https://doi.org/10.1262/jrd.18084
  19. S. T. Long, T. Nakao, S. Wakatake, M. Okakoi. Effect of CIDR 12 to 19 days after AI on detection of returning estrus and conception rate in dairy cows. The Journal of Reproduction and Development, no. 2, vol. 56, pp. 251-255, 2010. DOI: https://doi.org/10.1262/jrd.09-134t
  20. C. Azevedo, I. Maia, N. Canada, Simoes, J. Comparison of fertility, regular returns-to-estrus, and calving interval between Ovsynch and CO-synch + CIDR protocols in dairy cows. Theriogenology, no. 6, vol. 82, pp. 910-914, 2014. DOI: https://doi.org/10.1016/j.theriogenology.2014.07.006
  21. C. Stelletta, K. Tekin, M. B. Tirpan, H. Alemdar, B. Cil, F. Oztutar Stelletta, et al. Vulvar thermal pattern following synchronization of estrus is linked to fertility after timed artificial insemination in goat. Theriogenology, vol. 103, pp, 137-142, 2017. DOI: https://doi.org/10.1016/j.theriogenology.2017.07.038