Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Complete mitochondrial genome of Nyctalus aviator and phylogenetic analysis of the family Vespertilionidae

  • Lee, Seon-Mi (Animal Resources Division, National Institute of Biological Resources) ;
  • Lee, Mu-Yeong (DNA Analysis Division, Seoul Institute, National Forensic Service) ;
  • Kim, Sun-sook (Division of Basic Research, National Institute of Ecology) ;
  • Kim, Hee-Jong (Chungnam Wild Animal Rescue Center, Kongju National University) ;
  • Jeon, Hye Sook (Animal Resources Division, National Institute of Biological Resources) ;
  • An, Junghwa (Animal Resources Division, National Institute of Biological Resources)
  • Received : 2019.03.05
  • Accepted : 2019.07.16
  • Published : 2019.08.31
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Abstract

Bats influence overall ecosystem health by regulating species diversity and being a major source of zoonotic viruses. Hence, there is a need to elucidate their migration, population structure, and phylogenetic relationship. The complete mitochondrial genome is widely used for studying the genome-level characteristics and phylogenetic relationship of various animals due to its high mutation rate, simple structure, and maternal inheritance. In this study, we determined the complete mitogenome sequence of the bird-like noctule (Nyctalus aviator) by Illumina next-generation sequencing. The sequences obtained were used to reconstruct a phylogenic tree of Vespertilionidae to elucidate the phylogenetic relationship among its members. The mitogenome of N. aviator is 16,863-bp long with a typical vertebrate gene arrangement, consisting of 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 putative control region. Overall, the nucleotide composition is as follows: 32.3% A, 24.2% C, 14.3% G, and 29.2% T, with a slight AT bias (61.5%). The base composition of the 13 PCGs is as follows: 30.3% A, 13.4% G, 31.0% T, and 25.2% C. The phylogenetic analysis, based on 13 concatenated PCG sequences, infers that N. aviator is closely related to N. noctula with a high bootstrap value (100%).

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References

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