Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of the Stability of Poly-γ-Glutamate Hydrogels Prepared by UV and γ-Ray Irradiation

  • Park, Sang-Joon (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University) ;
  • Uyama, Hiroshi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Kwak, Mi-Sun (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University) ;
  • Sung, Moon-Hee (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University)
  • Received : 2018.12.11
  • Accepted : 2019.07.08
  • Published : 2019.07.28
Download PDF
⟨ Previous Next ⟩

Abstract

Poly-${\gamma}$-glutamate (${\gamma}$-PGA) has various applications due to its desirable characteristics in terms of safety and biodegradability. Previous studies have been conducted on ${\gamma}$-PGA hydrogels produced by ${\gamma}$-ray irradiation, but these hydrogels have proved unstable in solutions. This study was conducted to enable the ${\gamma}$-PGA hydrogel to maintain a stable form in solutions. The ${\gamma}$-PGA mixture for UV-irradiation was prepared with a cross-linker (N,N,N-trimethyl-3-[(2-methylacryloyl)amino]propan-1-aminium). Both ${\gamma}$-PGA hydrogels' characteristics, including stability in solutions, were examined. The UV-irradiated ${\gamma}$-PGA hydrogel maintained a stable form during the nine weeks of the study, but the ${\gamma}$-ray irradiated hydrogel dissolved after one week.

Keywords

References

  1. Giri TK, Thakur A, Alexander A, Badwaik H, Tripathi DK. 2012. Modified chitosan hydrogels as drug delivery and tissue engineering systems: present status and applications. Acta Pharm. Sin. B. 2: 439-449. https://doi.org/10.1016/j.apsb.2012.07.004
  2. Lee Y-H, Chang J-J, Yang M-C, Chien C-T, Lai W-F. 2012. Acceleration of wound healing in diabetic rats by layered hydrogel dressing. Carbohydr. Polym. 88: 809-819. https://doi.org/10.1016/j.carbpol.2011.12.045
  3. Azuma C, Yasuda K, Tanabe Y, Taniguro H, Kanaya F, Nakayama A, et al. 2007. Biodegradation of high-toughness double network hydrogels as potential materials for artificial cartilage. J. Biomed. Mater. Res. A. 81: 373-380.
  4. Pan L, Yu G, Zhai D, Lee HR, Zhao W, Liu N, et al. 2012. Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity. Proc. Natl. Acad. Sci. USA 109: 9287-9292. https://doi.org/10.1073/pnas.1202636109
  5. Salick DA, Kretsinger JK, Pochan DJ, Schneider JP. 2007. Inherent antibacterial activity of a peptide-based $\beta$-hairpin hydrogel. J. Am. Chem. Soc. 129: 14793-14799. https://doi.org/10.1021/ja076300z
  6. Thomas V, Yallapu MM, Sreedhar B, Bajpai S. 2007. A versatile strategy to fabricate hydrogel-silver nanocomposites and investigation of their antimicrobial activity. J. Colloid Interface Sci. 315: 389-395. https://doi.org/10.1016/j.jcis.2007.06.068
  7. Murakami S, Aoki N, Matsumura S. 2011. Bio-based biodegradable hydrogels prepared by crosslinking of microbial poly($\gamma$-glutamic acid) with L-lysine in aqueous solution. Nat. Polymer J. 43: 414-420. https://doi.org/10.1038/pj.2010.142
  8. Lee E-H, Kamigaito Y, Tsujimoto T, Seki S, Uyama H, Tagawa S, et al. 2010. Preparation of Poly ($\gamma$-glutamic acid) hydrogel/apatite composites and their application for scaffold of cell proliferation. J. Fiber Sci. Technol. 66: 104-111.
  9. Chung S, Gentilini C, Callanan A, Hedegaard M, Hassing S, Stevens MM. 2013. Responsive poly ($\gamma$-glutamic acid) fibres for biomedical applications. J. Mater. Chem. B. 1: 1397-1401. https://doi.org/10.1039/c3tb00515a
  10. Valliant EM, Romer F, Wang D, McPhail DS, Smith ME, Hanna JV, et al. 2013. Bioactivity in silica/poly ($\gamma$-glutamic acid) sol-gel hybrids through calcium chelation. Acta Biomater. 9: 7662-7671. https://doi.org/10.1016/j.actbio.2013.04.037
  11. Garcia JPD, Hsieh M-F, Doma BT, Peruelo DC, Chen I-H, Lee H-M. 2013. Synthesis of gelatin-$\gamma$-polyglutamic acid-based hydrogel for the in vitro controlled release of epigallocatechin gallate (EGCG) from Camellia sinensis. Polymers 6: 39-58. https://doi.org/10.3390/polym6010039
  12. Sung MH, Park C, Kim CJ, Poo H, Soda K, Ashiuchi M. 2005. Natural and edible biopolymer poly-gamma-glutamic acid: synthesis, production, and applications. Chem. Rec. 5: 352-366. https://doi.org/10.1002/tcr.20061
  13. Poo H, Park C, Kwak MS, Choi DY, Hong SP, Lee IH, et al. 2010. New biological functions and applications of high-molecular-mass Poly-$\gamma$-glutamic acid. Chem. Biodivers. 7: 1555-1562. https://doi.org/10.1002/cbdv.200900283
  14. Ho GH, Ho TI, Hsieh KH, Su YC, Lin PY, Yang J, et al. 2006. $\gamma$-Polyglutamic acid produced by Bacillus subtilis (Natto): structural characteristics, chemical properties and biological functionalities. J. Chin. Chem. Soc. 53: 1363-1384. https://doi.org/10.1002/jccs.200600182
  15. Li Z, He G, Hua J, Wu M, Guo W, Gong J, et al. 2017. Preparation of $\gamma$-PGA hydrogels and swelling behaviors in salt solutions with different ionic valence numbers. RSC Adv. 7: 11085-11093. https://doi.org/10.1039/C6RA26419K
  16. Choi S-H, Whang K-S, Park J-S, Choi W-Y, Yoon M-H. 2005. Preparation and swelling c harac teristic s of hydrogel from microbial poly ($\gamma$-glutamic acid) by $\gamma$-irradiation. Macromol. Res. 13: 339-343. https://doi.org/10.1007/BF03218463
  17. Uchida R, Sato T, Tanigawa H, Uno K. 2003. Azulene incorporation and release by hydrogel containing methacrylamide propyltrimenthylammonium chloride, and its application to soft contact lens. J. Control. Release 92: 259-264. https://doi.org/10.1016/S0168-3659(03)00368-7
  18. Baker JP, Blanch HW, Prausnitz JM. 1995. Swelling properties of acrylamide-based ampholytic hydrogels: comparison of experiment with theory. Polymer 36: 1061-1069. https://doi.org/10.1016/0032-3861(95)93608-O
  19. Zhang X, Colon LA. 2006. Evaluation of poly {-N-isopropylacrylamide-co-[3-(methacryloylamino) propyl] trimethylammonium} as a stationary phase for capillary electrochromatography. Electrophoresis 27: 1060-1068. https://doi.org/10.1002/elps.200500588
  20. Aleksey V. Kurdyumov, Dale G. Swan, et al. 2017. Photoactivatable Crosslinker. U.S. Patent No. 20170022375A1. Surmodies, Inc., Eden Prairie, MN, U.S
  21. Shiladitya SENGUPTA, Suresh Rameshlal CHAWRAI, Shamik GHOSH, Sumana GHOSH, Nilu JAIN, Suresh SADHASIVAM, et al. 2018. Treatments for Resistant Acne. U.S. Patent No. 20160346294A1. IN. New Delhi: Vyome Therapeutics Ltd.
  22. Dong Wang, Scott C. Miller, et al. 2005. Water-soluble polymeric bone-targeting drug delivery system. U.S. Patent No. 20050287114A1. University of Utah Research Foundation.
  23. Espinosa-Andrews H, Enriquez-Ramirez KE, Garcia-Marquez E, Ramirez-Santiago C, Lobato-Calleros C, Vernon-Carter J. 2013. Interrelationship between the zeta potential and viscoelastic properties in coacervates complexes. Carbohydr. Polym. 95: 161-166. https://doi.org/10.1016/j.carbpol.2013.02.053
  24. Gopinathan J, Noh I. 2018. Recent trends in bioinks for 3D printing. Biomater. Res. 22: 11. https://doi.org/10.1186/s40824-018-0122-1
  25. Ahn J-I, Kuffova L, Merrett K, Mitra D, Forrester JV, Li F, et al. 2013. Crosslinked collagen hydrogels as corneal implants: effects of sterically bulky vs. non-bulky carbodiimides as crosslinkers. Acta Biomater. 9: 7796-7805. https://doi.org/10.1016/j.actbio.2013.04.014
  26. Arakaki K, Kitamura N, Fujiki H, Kurokawa T, Iwamoto M, Ueno M, et al. 2010. Artificial cartilage made from a novel double-network hydrogel: in vivo effects on the normal cartilage and ex vivo evaluation of the friction property. J. Biomed. Mater. Res. A. 93: 1160-1168.

Cited by

  1. Biomedical Applications of Bacteria-Derived Polymers vol.13, pp.7, 2021, https://doi.org/10.3390/polym13071081
  2. From Residues to Added-Value Bacterial Biopolymers as Nanomaterials for Biomedical Applications vol.11, pp.6, 2019, https://doi.org/10.3390/nano11061492