Journal of Plant Biotechnology

  • 제46권2호
  • /
  • Pages.71-78
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  • 2019
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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참외 전장유전체 염기서열 분석 및 SSR 마커 개발

Whole genome re-sequencing and development of SSR markers in oriental melon

  • 송운호 (동국대학교 서울 생명과학과) ;
  • 정상민 (동국대학교 서울 생명과학과)
  • Song, Woon-Ho (Department of Life Science, Dongguk University-seoul) ;
  • Chung, Sang-Min (Department of Life Science, Dongguk University-seoul)
  • 투고 : 2019.04.09
  • 심사 : 2019.04.16
  • 발행 : 2019.06.30
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초록

The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

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참고문헌

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