Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects

  • Jo, Min-Ho (Department of Food Science Technology, Chonnam National University) ;
  • Hong, Seong-Jin (Department of Food Science Technology, Chonnam National University) ;
  • Lee, Ha-Nul (Department of Food Science Technology, Chonnam National University) ;
  • Ju, Jung-Hyun (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Bo-Ram (Department of Agro-food Resources National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Jun-ho (Department of Biotechnology, Chonnam National University) ;
  • Kim, Sun-Am (Jeonnam Bioindustry Foundation Bio Control Research Center) ;
  • Eun, Jong-Bang (Department of Food Science Technology, Chonnam National University) ;
  • Wee, Young-Jung (Department of Food Science and Technology, Yeungnam University) ;
  • Kim, Young-Min (Department of Food Science Technology, Chonnam National University)
  • Received : 2019.05.02
  • Accepted : 2019.06.03
  • Published : 2019.06.28
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Abstract

Gamma-aminobutyric acid (GABA)-producing strains were isolated from four edible insects and subjected to 16S rRNA sequence analysis. Among the four GABA-producing bacteria, Enterococcus avium JS-N6B4 exhibited the highest GABA-production, while cultivation temperature, initial pH, aerobic condition, and mono-sodium glutamate (MSG) feeding were found to be the key factors affecting GABA production rate. The culture condition was optimized in terms of glucose, yeast extract, and MSG concentrations using response surface methodology (RSM). GABA production up to 16.64 g/l was obtained under the conditions of 7 g/l glucose, 45 g/l yeast extract, and 62 g/l MSG through the optimization of medium composition by RSM. Experimental GABA production was 13.68 g/l, which was close to the predicted value (16.64 g/l) calculated from the analysis of variance, and 2.79-fold higher than the production achieved with basic medium. Therefore, GABA-producing strains may help improve the GABA production in edible insects, and provide a new approach to the use of edible insects as effective food biomaterials.

Keywords

References

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