한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제47권2호
  • /
  • Pages.195-200
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  • 2019
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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물여뀌 에탄올 추출물의 미백 효과

Whitening Activities of Ethanol Extract from Polygonum amphibium L.

  • 황병수 (국립낙동강생물자원관) ;
  • 이승영 (국립낙동강생물자원관) ;
  • 강창희 (국립낙동강생물자원관) ;
  • 한웅 (국립낙동강생물자원관) ;
  • 오영택 (국립낙동강생물자원관) ;
  • 유상미 (국립낙동강생물자원관) ;
  • 김민진 (국립낙동강생물자원관) ;
  • 김철환 (국립낙동강생물자원관) ;
  • 엄정혜 (국립낙동강생물자원관) ;
  • 정상철 (국립낙동강생물자원관) ;
  • 이욱재 (국립낙동강생물자원관) ;
  • 안영희 (국립낙동강생물자원관) ;
  • 정용태 (국립낙동강생물자원관)
  • Hwang, Buyng Su (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Seung Young (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Kang, Chang Hee (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Han, Woog (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Oh, Young Taek (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Yu, Sang Mi (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Min Jin (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Chul Hwan (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Eom, Jung Hye (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Sang Chul (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Wook Jae (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Ahn, Young Hee (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Yong Tae (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
  • 투고 : 2018.10.18
  • 심사 : 2018.11.12
  • 발행 : 2019.06.28
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초록

The purpose of this study was to investigate the melanogenesis inhibiting activity of the ethanol extract from Polygonum amphibium L. Firstly, the n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), n-butanol (BuOH), and water (Water) fractions were isolated from the P. amphibium L. ethanol extract. The efficacy of melanogenesis was found to significantly decrease via the EA and BuOH fractions when compared to the control in B16F10 cells. EA particularly showed the lowest melanin content in B16F10 cells when compared to all the other extracts. Concentration-dependent inhibition of melanin synthesis was also observed in the EA fraction at concentrations below $50{\mu}g/ml$, which did not exhibit cytotoxicity in B16F10 cells. Notably, the expression of three key proteins (tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2), which are involved in melanogenesis, were significantly decreased via the EA fraction. EA also inhibited body pigmentation in vivo in a zebrafish model. Overall, we demonstrated melanogenesis suppression using the EA fraction from P. amphibium L., which could be a potential candidate for an antimelanogenesis agent.

키워드

참고문헌

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