미생물학회지 (Korean Journal of Microbiology)

  • 제55권2호
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  • Pages.177-179
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  • 2019
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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중위도 산림토양에서 분리한 부식질 분해능이 있는 Pseudomonas kribbensis CHA-19의 유전체 염기서열 초안

Draft genome sequence of humic substances-degrading Pseudomonas kribbensis CHA-19 from temperate forest soil

  • 김덕규 (극지연구소 극지생명과학연구부) ;
  • 이형석 (극지연구소 극지유전체사업단)
  • Kim, Dockyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Hyoungseok (Unit of Polar Genomics, Korea Polar Research Institute)
  • 투고 : 2019.04.11
  • 심사 : 2019.05.08
  • 발행 : 2019.06.30
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초록

미국 뉴저지주 중위도 산림토양에서 부식산(천연 복합유기화합물인 부식질의 주요 구성성분) 분해능이 있는 세균 균주 Pseudomonas kribbensis CHA-19를 분리하였으며, 이후 또 다른 토양 유기물인 리그닌과 리그닌 유래의 페룰산(ferulic acid)과 바릴린산(vanillic acid)의 분해능을 확인하였다. 부식질 초기 저분자화 효소(예, dye-decolorizing peroxidase와 laccase-like multicopper oxidase)와 부식질 유래의 다양한 저분자 분해산물들을 분해하는 효소(예, vanillate O-demethylase와 biphenyl 2,3-dioxygenase)를 탐색하기 위해 CHA-19 게놈염기서열을 분석하였다. 최종 확보한 효소유전자 정보는 토양세균의 부식질 분해경로 제안에 사용되었다.

Pseudomonas kribbensis CHA-19 was isolated from a temperate forest soil (mid latitude) in New Jersey, USA, for its ability to degrade humic acids, a main component of humic substances (HS), and subsequently confirmed to be able to decolorize lignin (a surrogate for HS) and catabolize lignin-derived ferulic and vanillic acids. The draft genome sequence of CHA-19 was analyzed to discover the putative genes for depolymerization of polymeric HS (e.g., dye-decolorizing peroxidases and laccase-like multicopper oxidases) and catabolic degradation of HS-derived small aromatics (e.g., vanillate O-demethylase and biphenyl 2,3-dioxygenase). The genes for degradative activity were used to propose a HS degradation pathway of soil bacteria.

키워드

MSMHBQ_2019_v55n2_177_f0001.png 이미지

Fig. 1. Proposed HS-degradative pathway by Pseudomonas kribbensis CHA-19.

참고문헌

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