Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Complete genome sequence of Pantoea intestinalis SRCM103226, a microbial C40 carotenoid zeaxanthin producer

식용곤충 갈색거저리에서 분리한 카로테노이드 생성균주인 Pantoea intestinalis SRCM103226 균주의 유전체 해독

  • Kim, Jin Won (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Ha, Gwangsu (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Seong-Yeop (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Do-Youn (Microbial Institute for Fermentation Industry (MIFI))
  • 김진원 ((재)발효미생물산업진흥원) ;
  • 하광수 ((재)발효미생물산업진흥원) ;
  • 정성엽 ((재)발효미생물산업진흥원) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2019.03.25
  • Accepted : 2019.04.10
  • Published : 2019.06.30
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Abstract

Pantoea intestinalis SRCM103226, isolated from edible insect mealworm overproduces zeaxanthin as a main carotenoid. The complete genome of P. intestinalis SRCM103226 was sequenced using the Pacific Biosciences (PacBio) RS II platform. The genome of P. intestinalis SRCM103226 comprises a 4,784,919 bp circular chromosome (53.41% G+C content), and is devoid of any extrachromosomal plasmids. Annotation using the RAST server reveals 4,332 coding sequences and 107 RNAs (22 rRNA genes, 85 tRNA genes). Genome annotation analysis revealed that it has five genes involved in the carotenoid pathway. The genome information provides fundamental knowledge for comparative genomics studies of the zeaxanthin pathway.

Pantoea intestinalis SRCM103226은 식용곤충 밀웜으로부터 분리하였으며, zeaxanthin을 메인으로 생산하였다. P. intestinalis SRCM103226의 유전체 분석을 실시하여 4,784,919 bp 크기의 염기서열, GC 비율은 53.41%로 나타났으며, 플라스미드는 존재하지 않는다. RAST server를 이용하여 annotation한 결과 4,332개의 코딩유전자, 22개의 rRNA, 85개의 tRNA 유전자가 확인되었다 지놈분석결과 zeaxanthin 생합성회로 5개 유전자를 가지고 있다. 이러한 유전체 정보는 zeaxanthin 생합성 경로의 분자 진화의 비교 유전체학 연구에 대한 기초 정보를 제공한다.

Keywords

MSMHBQ_2019_v55n2_167_f0001.png 이미지

Fig. 1. Graphical Circular map of Pantoea intestinalis SRCM103226.

MSMHBQ_2019_v55n2_167_f0002.png 이미지

Fig. 2. Carotenoid profile of Pantoea intestinalis SRCM103226.

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Fig. 3. C40 carotenoid zeaxanthin biosynthesis gene cluster.

Table 1. General genomic features of Pantoea intestinalis SRCM103226

MSMHBQ_2019_v55n2_167_t0001.png 이미지

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