미생물학회지 (Korean Journal of Microbiology)

  • 제55권2호
  • /
  • Pages.112-116
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  • 2019
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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분열효모에서 Thp1/PCID2의 이종상동체인 SPAC1B3.08이 mRNA 방출에 미치는 영향

Effects of SPAC1B3.08, ortholog of Thp1/PCID2, on mRNA export in fission yeast

  • 박진희 (성신여자대학교 바이오생명공학과) ;
  • 윤진호 (성신여자대학교 바이오생명공학과)
  • Park, Jin Hee (Department of Biotechnology, Sungshin Women's University) ;
  • Yoon, Jin Ho (Department of Biotechnology, Sungshin Women's University)
  • 투고 : 2019.05.27
  • 심사 : 2019.06.05
  • 발행 : 2019.06.30
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초록

Thp1/PCID2 단백질은 전사와 연계되어 mRNA를 핵에서 세포질로 방출하는데 필요한, 진화적으로 보존된 TREX-2 복합체의 구성요소이다. 분열효모인 Schizosaccharomyces pombe에는 Thp1/PCID2 단백질의 이종상동체가 2개 존재한다. pci2(SPBC1105.07c) 유전자 이외에, SPAC1B3.08 유전자는 PCI 영역을 갖고 있으며 TREX-2 복합체의 구성요소로 추정되는 단백질을 암호화하고 있다. SPAC1B3.08을 과발현하면, 생장이 약간 느려지고 mRNA 방출에 결함을 보였다. Yeast two-hybrid와 공동침전 분석 실험에서 SPAC1B3.08 단백질은 TREX-2 복합체의 다른 구성요소인 Sac3, Dss1 단백질들과 상호작용하였다. 이와 같은 관찰들은 S. pombe의 SPAC1B3.08 단백질도 TREX-2 복합체의 구성요소로서 mRNA 방출에 관여할 가능성을 지지한다.

Thp1/PCID2 is a subunit of the evolutionally conserved TREX-2 complex, which is required for transcription-coupled mRNA export from the nucleus to the cytoplasm. In fission yeast, Schizosaccharomyces pombe, there are two orthologs of the Thp1/PCID2 protein. In addition to pci2 (SPBC1105.07c) gene, SPAC1B3.08 gene encodes a PCI domain-containing protein that is predicted as a component of TREX-2 complex. Overexpression of SPAC1B3.08 cause slight defects of both growth and mRNA export. Yeast two-hybrid and co-immunoprecipitation analysis exhibits that the SPAC1B3.08 protein interacted with Sac3 and Dss1, which are another components of TREX-2 complex. These observations support the possibility that the S. pombe SPAC1B3.08 protein, as a component of TREX-2 complex, is involved in mRNA export.

키워드

MSMHBQ_2019_v55n2_112_f0001.png 이미지

Fig. 1. Phylogenetic tree of PCID2/Thp1 proteins.

MSMHBQ_2019_v55n2_112_f0002.png 이미지

Fig. 2. Over-expression of SPAC1B3.08 cause slight growth retardation and the defects in mRNA export.

MSMHBQ_2019_v55n2_112_f0003.png 이미지

Fig. 3. Physical interaction of SPAC1B3.08 with Sac3 in S. pombe extracts.

Table 1. Yeast two-hybrid analysis

MSMHBQ_2019_v55n2_112_t0001.png 이미지

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