Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

  • Zhen, Ao Xuan (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Piao, Mei Jing (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Hyun, Yu Jae (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Kang, Kyoung Ah (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Ryu, Yea Seong (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Cho, Suk Ju (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Kang, Hee Kyoung (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Koh, Young Sang (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine) ;
  • Ahn, Mee Jung (Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University) ;
  • Hyun, Jin Won (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
  • Received : 2018.08.07
  • Accepted : 2018.10.06
  • Published : 2019.07.01
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Abstract

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 ($PM_{2.5}$) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and $PM_{2.5}$ severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or $PM_{2.5}$. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and $PM_{2.5}$.

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References

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