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저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석

Isolation and Characterization of Five Isolates of Tetraselmis sp. with Rapid Growth Rates in Low Temperatures

  • 박한울 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 허동희 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 신동우 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 김지훈 (국립낙동강생물자원관) ;
  • 홍성주 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 임상민 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 이철균 (해양바이오에너지 생산기술개발연구센터, 인하대학교)
  • 투고 : 2019.05.30
  • 심사 : 2019.06.18
  • 발행 : 2019.06.30

초록

For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures ($5-15^{\circ}C$). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at $10^{\circ}C$ were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.

키워드

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Figure 1. Phylogenetic Analysis of the 18S rDNA of New Tetraselmis sp. Isolates.

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Figure 2. Time Profiles of Biomass Concentrations of Tetraselmis sp. Strains at (a) 5°C, (b) 10°C, and (c) 15°C.

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Figure 3. Biomass Productivities by the Microalgal Strains and Temperature.

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Figure 4. Fatty Acid Contents of the Microalgae at 10°C.

Table 1. Sequences of Primers Used for Amplification of 18S rDNA

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Table 2. Fatty Acid Compositions of the Microalgae.

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참고문헌

  1. Jay, A., Reidmiller, D.R., Avery, C.W., Barrie, D., DeAngelo, B.J., Dave, A., Dzaugis, M., Kolian, M., Lewis, K.L.M., Reeves, K., and Winner, D. 2018. Overview. In Impacts, Risks, and Adaptation in the United States. Fourth National Climate Assessment. 2, 33-71.
  2. Jung, S.G., Kim, S.K., Bun, M.S., Cho, Y., Shin, D.W., Kim, Z-H., Lim, S.M., and Lee, C.G. 2016. Comparison of Biomass Productivity of the Microalgae, Tetraselmis sp. KCTC12236BP, in Polyvinyl Chloride Marine Photobioreactor and High Density Polyethylene Marine Photobioreactor. J. Mar. Biosci. Biotechnol. 8, 18-23. https://doi.org/10.15433/ksmb.2016.8.1.018
  3. Kalghatgi, G. 2018. Is it really the end of internal combustion engines and petroleum in transport? Appl. Energ. 225, 965-974 https://doi.org/10.1016/j.apenergy.2018.05.076
  4. Kim, C.W. and Hur, S.B. 1998. Selection of Optimum Species of Tetraselmis for Mass Culture. J. Aquaculture. 11, 231-240.
  5. Park, H., Jung, D., Lee, J., Kim, P., Cho, Y., Jung, I., Kim, Z.H., Lim, S.M. and Lee, C.G. 2018. Improvement of biomass and fatty acid productivity in ocean cultivation of Tetraselmis sp. using hypersaline medium. J. Appl. Phycol. 30, 2725-2735. https://doi.org/10.1007/s10811-018-1388-3
  6. Park, H., Lee C.G. 2016. Theoretical calculations on the feasibility of microalgal biofuels: utilization of marine resources could help realizing the potential of microalgae. Biotechnol. J. 11, 1461-1470. https://doi.org/10.1002/biot.201600041
  7. Popp, J., Lakner, Z., Harangi-Rakos, M. and Fari, M. 2014. The effect of bioenergy expansion: food, energy, and environment. Renew. and Sust. Energ. Rev. 32, 559-578. https://doi.org/10.1016/j.rser.2014.01.056
  8. Sakamoto, T. and Murata, N. 2002. Regulation of the desaturation of fatty acids and its role in tolerance to cold and salt stress. Curr. Opin. Microbiol. 5, 206-210. https://doi.org/10.1016/S1369-5274(02)00306-5
  9. Shin, D.W., Bae, J.H., Cho, Y.H., Kim, Z-H., Lim, S.M., and Lee, C.G. 2016. Isolation of New Microalga, Tetraselmis sp. KCTC12236BP, and Biodiesel Production using Its Biomass. J. Mar. Biosci. Biotechnol. 8, 39-44. https://doi.org/10.15433/ksmb.2016.8.1.039
  10. Teoh, M.L., Chu, W.L., Marchant, H. and Phang, S.M. 2004. Influence of culture temperature on the growth, biochemical composition and fatty acid profiles of six Antarctic microalgae. J. Appl. Phycol. 16, 421-430. https://doi.org/10.1007/s10811-004-5502-3

피인용 문헌

  1. Year-Round Cultivation of Tetraselmis sp. for Essential Lipid Production in a Semi-Open Raceway System vol.19, pp.6, 2021, https://doi.org/10.3390/md19060314