식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제49권2호
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  • Pages.127-139
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  • 2019
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
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Exploring natural hybridizations among Asplenium ruprechtii and related taxa in Korea

  • 투고 : 2019.05.30
  • 심사 : 2019.06.21
  • 발행 : 2019.06.30
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초록

The purported four hybrid origins of Asplenium in Korea were tested based on morphological, cytological and DNA sequence data. Asplenium castaneo-viride, A. ${\times}$ uiryeongse, A. ${\times}$ montanus, and A. ${\times}$ kitazawae share several morphological characteristics with the Asian walking fern A. ruprechtii and related taxa as parents and show a sympatric distribution with the putative parents, raising the possibility of hybrid origins: A. castaneo-viride (A. ruprechtii and A. incisum), A. ${\times}$ uiryeongse (A. ruprechtii and A. pekinense), A. ${\times}$ montanus (A. ruprechtii, A. trichomanes, and A. incisum), and A. ${\times}$ kitazawae (A. ruprechtii and A. sarelii). We investigated flow cytometry and chloroplast DNA sequence data (rbcL, rps4-trnS, and rps4-trnS intergenic spacer) to clarify the hybridization and origin of each hybrid. In the flow cytometry analyses, A. ruprechtii shows diploid (2x) only, whereas A. castaneo-viride (3x, 4x), A. ${\times}$ uiryeongse (3x), A. ${\times}$ montanus (3x, 4x), and A. ${\times}$ kitazawae (2x, 4x) exhibit polyploidy, suggesting hybrid events along speciation. The rbcL and rps4-trnS and rps4-trnS intergenic spacer data suggest that A. ruprechtii is one the maternal ancestors of all four hybrids. In addition, the rps4-trnS and rps4-trnS intergenic spacer data indicate that A. incisum is also the maternal ancestor of A. ${\times}$ kitazawae and A. ${\times}$ montanus, proposing multiple hybridization events for these two hybrids. In A. ${\times}$ montanus, morphological features such as the leaf forms and sympatric distributions of the species also support the multimaternal hypothesis, but the morphological features of A. ${\times}$ kitazawae must be examined with consideration of hybrid events. To clarify the complex hybrid evolutionary lineages of the four Asplenium hybrids, further research with taxon sampling and molecular markers should be conducted.

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참고문헌

  1. Anderson, E. 1949. Introgressive Hybridization. Wiley, New York, 109 pp.
  2. Arnold, M. L. 1997. Natural Hybridization and Evolution. Oxford University Press, New York, 232 pp.
  3. Ceccarelli, M., S. Minelli, F. Maggini and P. G. Cionini. 1995. Genome size variation in Vicia faba. Heredity 74: 180-187. https://doi.org/10.1038/hdy.1995.26
  4. Chang, Y., J. Li, S. Lu and H. Schneider. 2013. Species diversity and reticulate evolution in the Asplenium normale complex (Aspleniaceae) in China and adjacent areas. Taxon 62: 673-687. https://doi.org/10.12705/624.6
  5. Ching, R.-C. and K. Iwatsuki. 1982. Annotations and corrigenda on the Sino-Japanese pteridophytes (I). The Journal of Japanese Botany 57: 129-132.
  6. Chung, K.-S., A. L. Hipp and E. H. Roalson. 2012. Chromosome number evolves independently of genome size in a clade with nonlocalized centromeres (Carex: Cyperaceae). Evolution 66: 2708-2722. https://doi.org/10.1111/j.1558-5646.2012.01624.x
  7. Cronn, R., R. L. Small, T. Haselkorn and J. F. Wendel. 2003. Cryptic repeated genomic recombination during speciation in Gossypium gossypioides. Evolution 57: 2475-2489. https://doi.org/10.1111/j.0014-3820.2003.tb01493.x
  8. Dolezel, J. and J. Bartos. 2005. Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany 95: 99-110. https://doi.org/10.1093/aob/mci005
  9. Ebihara, A., H. Ishikawa, S. Matsumoto, S.-J. Lin, K. Iwatsuki, M. Takamiya, Y. Watano and M. Ito. 2005. Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent areas. American Journal of Botany 92: 1535-1547. https://doi.org/10.3732/ajb.92.9.1535
  10. Ekrt, L. and M. Stech. 2008. A morphometric study and revision of the Asplenium trichomanes group in the Czech Republic. Preslia 80: 325-347.
  11. Ellstrand, N. C., L. C. Garner, S. Hegde, R. Guadagnuolo and L. Blancas. 2007. Spontaneous hybridization between maize and teosinte. Journal of Heredity 98: 183-187. https://doi.org/10.1093/jhered/esm002
  12. Farris J. S., M. Kallersjo, A. G. Kluge and C. Bult. 1994. Testing significance of incongruence. Cladistics 10: 315-319. https://doi.org/10.1111/j.1096-0031.1994.tb00181.x
  13. Farris J. S., M. Kallersjo, A. G. Kluge and C. Bult. 1995. Constructing a significance test for incongruence. Systematic Biology 44: 570-572. https://doi.org/10.1093/sysbio/44.4.570
  14. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  15. Gibson, T., D. Higgins and J. Thompson. 1994. Clustal X Program. EMBL, Heidelberg, Germany.
  16. Grant, V. 1981. Plant Speciation. Columbia University Press, New York, 432 pp.
  17. Hasebe, M., P. G. Wolf, K. M. Pryer, K. Ueda, M. Ito, R. Sano, G. J. Gastony, J. Yokoyama, J. R. Manhart, N. Murakami, E. H. Crane, C. H. Haufler and W. D. Hauk. 1995. Fern phylogeny based on rbcL nucleotide sequences. American Fern Journal 85: 134-181. https://doi.org/10.2307/1547807
  18. Hennequin, S., A. Ebihara, M. Ito, K. Iwatsuki and J.-Y. Dubuisson. 2003. Molecular systematics of the fern genus Hymenophyllum s.l. (Hymenophyllaceae) based on chloroplastic coding and noncoding regions. Molecular Phylogenetics and Evolution 27: 283-301. https://doi.org/10.1016/S1055-7903(02)00404-9
  19. Iwatsuki, K. 1995. Aspleniaceae. In Flora of Japan. Vol. 1. Iwatsuki, K., T. Yamazaki, D. E. Boufford and H. Ohba (eds.), Kodansha Ltd., Tokyo, 302 pp.
  20. Jessen, S. 1995. Asplenium trichomanes subsp. hastatum, stat. nov. - eine neue Unterate des Braunstiel-Streifenfarnes in Europa und vier neue intraspezifische Hybriden (Aspleniaceae, Pteridophyta). Berichte der Bayerischen Botanischen Gesellschaft zur Erforschung der heimischen Flora 65: 107-132.
  21. Kim, C. H. and B.-Y. Sun. 2007. Aspleniaceae. In The Genera of Vascular Plants of Korea. Park, C.-W. (ed.), Academy Publishing Co., Seoul, 1482 pp.
  22. Kwon, Y. J., C. H. Kim, J. K. Ahn and B.-Y. Sun. 2009. Analysis of hybridity of Asplenium castaneo-viride Baker. Korean Journal of Plant Taxonomy 39: 12-23. https://doi.org/10.11110/kjpt.2009.39.1.012
  23. Lee, C. S. and K. Lee. 2015. Pteridophytes: Lycophytes & Ferns. Geobook, Seoul, 471 pp. (in Korean)
  24. Lee, C. S. and K. Lee. 2018. Pteridophytes: Lycophytes & Ferns. 2nd. Geobook, Seoul, 491 pp. (in Korean)
  25. Lee, C. S., K. Lee., S. H. Yeau and K.-S. Chung. 2015. Two new and one unrecorded natural hybrids between Asplenium ruprechtii and related taxa (Aspleniaceae). Korean Journal of Plant Taxonomy 45: 362-368. https://doi.org/10.11110/kjpt.2015.45.4.362
  26. Lee, C. S., S.-C. Kim, S. H. Yeau and N. S. Lee. 2012. Nuclear and cpDNA sequences demonstrate spontaneous hybridization between Goodyera schlechtendaliana Rchb. f. and G. velutina Maxim. (Orchidaceae) in Jeju Island, Korea. Systematic Botany 37: 356-364.
  27. Lee, T. B. 1980. Illustrated Flora of Korea. Hyangmunsa, Seoul, 990 pp. (in Korean)
  28. Lee, Y. N. 2006. New Flora of Korea. Kyohaksa Publ. Co., Seoul, Vol. 1, 975 pp. (in Korean)
  29. Li, C.-X. and S.-G. Lu. 2006. Relationship of Asplenium yunnanense and A. lushanense inferred from the sequence analysis of chloroplast rbcL, trnL-F and rps4-trnS. Acta Phytotaxonomica Sinica 44: 296-303. https://doi.org/10.1360/aps040100
  30. Lin, Y. X. and R. Viane. 2012. Aspleniaceae. In Flora of China. Vol. 2. Wu, Z.-Y., P. H. Raven and D.-Y. Hong (eds.), Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, MO. Pp. 267-316.
  31. Lovis, J. D. and T. Reichstein. 1985. Asplenium trichomanes subsp. pachyrhachis (Aspleniaceae, Pteridophyta) and a note on the typication of A. trichomanes. Willdenowia 15: 187-201.
  32. Lovis, J. D., P. J. Brownsey, A. Sleep and M. G. Shivas. 1972. The origin of Asplenium balearicum. The British Fern Gazette 10: 263-268.
  33. Murakami, N. and B. A. Schaal. 1994. Chloroplast DNA variation and the phylogeny of Asplenium sect. Hymenasplenium (Aspleniaceae) in the New World tropics. Journal of Plant Research 107: 245-251. https://doi.org/10.1007/BF02344251
  34. Murakami, N., S. Nogami, M. Watanabe and K. Iwatsuki. 1999. Phylogeny of Aspleniaceae inferred from rbcL nucleotide sequences. American Fern Journal 89: 232-243. https://doi.org/10.2307/1547233
  35. Park, M. K. 1975. Illustrated Encyclopedia of Fauna and Flora of Korea. Vol. 16. Pteridophyta. Ministry of Education, Seoul, 549 pp. (in Korean)
  36. Perrie, L. R. and P. J. Brownsey. 2005. Insights into the biogeography and polyploid evolution of New Zealand Asplenium from chloroplast DNA sequence data. American Fern Journal 95: 1-21. https://doi.org/10.1640/0002-8444(2005)095[0001:IITBAP]2.0.CO;2
  37. Rieseberg, L. H. 1995. The role of hybridization in evolution: old wine in new skins. American Journal of Botany 82: 944-953. https://doi.org/10.1002/j.1537-2197.1995.tb15711.x
  38. Rieseberg, L. H., N. C. Ellstrand and M. Arnold. 1993. What can molecular and morphological markers tell us about plant hybridization? Critical Reviews in Plant Sciences 12: 213-241. https://doi.org/10.1080/07352689309701902
  39. Rumsey, F., S. Russell, H. Schafer and H. Rasbach. 2004. Distribution, ecology and cytology of Asplenium azoricum Lovis, Rasbach & Reichstein (Aspleniaceae, Pteridophyta) and its hybrids. American Fern Journal 94: 113-125. https://doi.org/10.1640/0002-8444(2004)094[0113:DEACOA]2.0.CO;2
  40. Schneider, H., S. J. Russell, C. J. Cox, F. Bakker, S. Henderson, F. Rumsey, J. Barrett, M. Gibby and J. C. Vogel. 2004a. Chloroplast phylogeny of Asplenioid ferns based on rbcL and trnL-F spacer sequences (Polypodiidae, Aspleniaceae) and its implications for biogeography. Systematic Botany 29: 260-274. https://doi.org/10.1600/036364404774195476
  41. Schneider, H., A. R. Smith, R. Cranfill, T. J. Hildebrand, C. H. Haufler and T. A. Ranker. 2004b. Unraveling the phylogeny of polygrammoid ferns (Polypodiaceae & Grammitidaceae): exploring aspects of the diversification of epiphytic plants. Molecular Phylogenetics and Evolution 31: 1041-1063. https://doi.org/10.1016/j.ympev.2003.09.018
  42. Shepherd, L. D., B. R. Holland and L. R. Perrie. 2008. Conflict amongst chloroplast DNA sequences obscures the phylogeny of a group of Asplenium ferns. Molecular Phylogenetics and Evolution 48: 176-187. https://doi.org/10.1016/j.ympev.2008.02.023
  43. Skog, J. E., J. T. Mickel, R. C. Moran, M. Volovsek and E. A. Zimmer. 2004. Molecular studies of representative species in the fern genus Elaphoglossum (Dryopteridaceae) based on cpDNA sequences rbcL, trnL-F, and rps4-trnS. International Journal of Plant Sciences 165: 1063-1075. https://doi.org/10.1086/423877
  44. Smith, A. R. and R. B. Cranfill. 2002. Intrafamilial relationships of the thelypteroid ferns (Thelypteridaceae). American Fern Journal 92: 131-149. https://doi.org/10.1640/0002-8444(2002)092[0131:IROTTF]2.0.CO;2
  45. Souza-Chies, T. T., G. Bittar, S. Nadot, L. Carter, E. Besin and B. Lejeune. 1997. Phylogenetic analysis of Iridaceae with parsimony and distance methods using the plastid gene rps4. Plant Systematics and Evolution 204: 109-123. https://doi.org/10.1007/BF00982535
  46. Suda, J. and P. Travnicek. 2006. Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry: new prospects for plant research. Cytometry 69A: 273-280. https://doi.org/10.1002/cyto.a.20253
  47. Suda, J., A. Krahulcova, P. Travnicek and F. Krahulec. 2006. Ploidy level versus DNA ploidy level: an appeal for consistent terminology. Taxon 55: 447-450. https://doi.org/10.2307/25065591
  48. Swofford, D. L. 2002. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.01b10. Sinauer Associates, Sunderland, MA, USA.
  49. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins. 1997. The CLUSTAL_X Windows Interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  50. Wang, Z., K. Wang, F. Zhang and Z. Hou. 2003. A biosystematics study of Asplenium sarelii complex. Acta Boyanica Sinica 45: 1-14.
  51. Wei, R., H. Schneider and X.-C. Zhang. 2013. Toward a new circumscription of the twinsorus-fern genus Diplazium (Athyriaceae): a molecular phylogeny with morphological implications and infrageneric taxonomy. Taxon 62: 441-457. https://doi.org/10.12705/623.8
  52. White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Innis M. A., D. H. Gelfand, J. J. Sninsky and T. J. White (eds.), Academic Press, San Diego, CA. Pp. 315-322.
  53. Yan, J., J. Zhang, K. Sun, D. Chang, S. Bai, Y. Shen, L. Huang, J. Zhang, Y. Zhang and Y. Dong. 2016. Ploidy level and DNA content of Erianthus arundinaceus as determined by flow cytometry and the association with biological characteristics. PLoS ONE 11: e0151948. https://doi.org/10.1371/journal.pone.0151948