양봉농가에서 생산된 프로폴리스 급여에 따른 꿀벌 질병의 저감효과

Reduction effect of bee disease caused by propolis feeding in beekeeping farm

Abstract

The study was conducted to investigate the effect of propolis on the increase of the immunity of honeybee bees by gradually administering the bees' propolis to bees. 0.16% and 0.3% 0.6% Propolis were administered at intervals of 2 weeks for 2 months, respectively, and 16 kinds of representative diseases of bees were tested. As a result, less bee disease was observed directly in the 0.3% propolis-administered group. In the next year, bees and a newborn bee showed a decrease in the incidence of bee disease and American foulbrood in bees administered with propolis. Based on the results of these studies, it was confirmed that propolis administration in bees helps to enhance immunity of bees and is effective in controlling American foulbrood.

Fig. 1. 0.3% propolis supply.

Fig. 2. 0.16% and 0.6% propolis supply.

Fig. 3. View of propolis in beehive.

Fig. 4. Propolis.

Fig. 5. Design of Experiment.

Fig. 6. Korea monthly average temperature graph for 2018.

Fig. 7. 2% agarose gel electrophorosis of PCR Picture (2018. 9. 28.). Sample 1: 0.16%propolis, 2: 0.3%propolis, 3: 0.6%propolis, 4: antibiotic, 5, 6: blank, 7: negative control.

Fig. 8. 2% agarose gel electrophorosis of PCR Picture (2019. 3. 11.) : PCR 1, PCR 2. 2% agarose gel electrophorosis of PCR Picture (2019. 4. 2.) : PCR 3, PCR 4. Sample 1: Blank, 2: 0.16%propolis, 3: 0.3%propolis, 4: 0.6%propolis, 5: negative control.

Table 1. List of sixteen bee diseases tested in this study

Table 2. Disease test results after administration of propolis by concentration