Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Ecogenetical Characteristics of Dolichospermum in Bukhan River

북한강 수계에서 Dolichospermum의 유전생태학적 특성 연구

  • Yu, Mi Na (Han River Environmental Research Center, National Institute of Environmental Research) ;
  • Byun, Jeong-Hwan (Han River Environmental Research Center, National Institute of Environmental Research) ;
  • Baek, Jun Soo (Han River Environmental Research Center, National Institute of Environmental Research) ;
  • Youn, Seok Jea (Han River Environmental Research Center, National Institute of Environmental Research) ;
  • Yu, Soon-ju (Han River Environmental Research Center, National Institute of Environmental Research) ;
  • Byeon, Myeong Seop (Han River Environmental Research Center, National Institute of Environmental Research)
  • 유미나 (국립환경과학원 한강물환경연구소) ;
  • 변정환 (국립환경과학원 한강물환경연구소) ;
  • 백준수 (국립환경과학원 한강물환경연구소) ;
  • 윤석제 (국립환경과학원 한강물환경연구소) ;
  • 유순주 (국립환경과학원 한강물환경연구소) ;
  • 변명섭 (국립환경과학원 한강물환경연구소)
  • Received : 2018.10.24
  • Accepted : 2018.12.14
  • Published : 2019.01.30
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Abstract

This study analyzed the occurrence pattern of Dolichospermum (= Anabaena) in the Bukhan river from March 2012 to December 2014 in order to identify the genotypes of Dolichospermum. Furthermore, 16S rRNA were analyzed to identify the genotypes of Dolichospermum that occurred in 2015 which were then compared to the reference sequence deposited at NCBI. During this period, the occurrence of Dolichospermum was highly correlated to water temperature. In the year 2012 and 2013, Dolichospermum appeared in Lake Cheongpyeong (CP), Sambong (SB), and Lake Paldang (P2) between July and August. However, in 2014, it appeared in SB and P2, but not in CP. This reduction in appearance was attributed to the decreased inflow to Lake Uiam as a result of low rainfall in 2014 as compared to 2012. In July 2015, the Dolichospermum 16S rRNA genotype was confirmed in five locations; Lake Cheongpyeong (CP), Seojong (SJ), Songchon Sewage Treatment Plant (SC), Joan (P4), and Lake Paldang (PD). Anabaena crassa of spiral clone, A. planctonica of linear clone, and A. circinalis of spiral clone exhibited high genetic similarity with the reference sequence. The 16r RNA genotype showed approximately 3 % sequence variation between the locations and were more similar to each other in locations that were closer.

Keywords

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Fig. 1. Map showing the sampling sites in Bukhan river.

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Fig. 2. Relationship between water temperature and Dolichospermum cell density: (a) Cheongpyeong (CP),(b) Sambong (SB), (c) Paldang (P2).

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Fig. 3. Phylogenetic relationships of Dolichospermum taxa using neighbor-joining tree algorithm. The algorithm was based on partial 16S rRNA sequence (699 bp). The Clones used were isolated single Dolichospermum cell from Bukhan river.

Table 1. Annual rainfall and number of rainy days in Chun-Cheon

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Table 2. Sequence comparisons among Dolichospermum with similar morphology

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