한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제35권3호
  • /
  • Pages.201-208
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  • 2019
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  • 2289-0971(pISSN)
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  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
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Strain YG-02가 생산하는 생물응집제의 성분 분석

The Component Analysis of the Bioflocculant Produced by Strain YG-02

  • 정연곤 (전남대학교 환경에너지공학과) ;
  • 고준일 (전남대학교 환경에너지공학과) ;
  • 정선용 (전남대학교 환경에너지공학과)
  • Jung, Yeon-Gon (Department of Environment and Energy Engineering, Chonnam National University) ;
  • Ko, Joon-Il (Department of Environment and Energy Engineering, Chonnam National University) ;
  • Chung, Seon-Yong (Department of Environment and Energy Engineering, Chonnam National University)
  • 투고 : 2019.02.14
  • 심사 : 2019.04.09
  • 발행 : 2019.05.30
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초록

In this study, we analyzed composition of the bioflocculant, which strain YG-02 produces. First, supernatant and suspension from centrifugation of culture fluid of the strain, were used in the flocculation experiment. As a result, the SVI(sludge volume index) added with the suspension, was 182 mL/g, same as the control group with no additive, and the SVI added with supernatant, was 164 mL/g. So, the result above showed that flocculation capacity of the bioflocculant, was dependent on the substance which strain YG-02 produces, not on factors such as the body of germs. As a result of the thermostability test on substances that cause flocculation, the flocculation effect was significantly reduced, compared to the result of the flocculation test, before applying heat to the culture fluid, and it was able to assume that the substance that causes flocculation, was damaged by heat. Additionally, to understand the component of the bioflocculant, analyzation of sugar composition and fatty acid, was conducted. As a result, sugar composition was the polysaccharide consisting of glucose: lactose with molar ratio of 90.75:9.25. Fatty acid content was detected, as 0.0012 g/100g, showing that it contained glycolipid in the bioflocculant. Such results show that the bioflocculant which strain YG-02 produces, is the new bioflocculant, different from bioflocculantstudiedto date.

키워드

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Fig. 1. Phylogenetic neighbor-joining tree of strain YG-02.

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Fig. 2. Preparation of culture fluid hydrolysate.

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Fig. 3. Comparison of sludge flocculation, added with centrifugated culture fluid. (A : Start, B : 30 minutes later, a : Control, b : The sludge added with the suspension, c : The sludge added with the supernatant)

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Fig. 4. Comparison of sludge flocculation, added with heated culture fluid. (A : Start, B : 30 minutes later, a : Control, b : The sludge added with the supernatant)

Table 1. Composition of R2A

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Table 2. SV30 and SVI, obtained from the flocculation experiment

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Table 3. SV30 and SVI, obtained from the flocculation experiment.

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Table 4. Comparison table of component sugars of flocculants produced by microorganisms

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Table 5. Result of fatty acid analysis.

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Table 6. Status of sludge treatment in D Gun, Jeollanam-do (2018)

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Table 7. Compost production status using sludge generated in D Gun, Jeollanam-do (2018)

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