Journal of Animal Science and Technology

  • 제61권2호
  • /
  • Pages.69-76
  • /
  • 2019
  • /
  • 2672-0191(pISSN)
  • /
  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
권호 표지
DOI QR코드

DOI QR Code

Study on the fatty acid profile of phospholipid and neutral lipid in Hanwoo beef and their relationship to genetic variation

  • Beak, Seok-Hyeon (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Lee, Yoonseok (Department of Biotechnology, College of Agriculture & Life Science, HanKyong National University) ;
  • Lee, Eun Bi (Institute of Eco-friendly Economic Livestock, Institute of Green-Bio Science & Technology, Seoul National University) ;
  • Kim, Kyoung Hoon (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Kim, Jong Geun (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Bok, Jin Duck (Institute of Eco-friendly Economic Livestock, Institute of Green-Bio Science & Technology, Seoul National University) ;
  • Kang, Sang-Kee (Graduate School of International Agricultural Technology, Seoul National University)
  • 투고 : 2018.12.12
  • 심사 : 2019.03.11
  • 발행 : 2019.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Maize which has very high omega-6 fatty acid content has been used as a main feed grain for Hanwoo beef production to increase marbling, and thus omega-6 to omega-3 fatty acids ratio in Hanwoo beef is expected to be biased. To elucidate the current status of omega fatty acids ratio in Hanwoo beef, fatty acid profiles of neutral lipid and phospholipid fraction were analyzed separately using 55 Hanwoo steers' longissimus dorsi muscle slaughtered at Pyeongchang, Korea from Oct. to Nov. 2015. In addition, an association study was conducted to evaluate associations between single nucleotide polymorphism (SNP) markers from references and omega fatty acid profiles in phospholipid of Hanwoo beef samples using analysis of variance (ANOVA). In neutral lipid fraction, composition of saturated and monounsaturated fatty acids was higher and polyunsaturated fatty acids was lower compared to those in phospholipid fraction. The mean n-6/n-3 ratios of Hanwoo were $56.059{\pm}16.180$ and $26.811{\pm}6.668$ in phospholipid and neutral lipid, respectively. There were three SNPs showing statistically significant associations with omega fatty acid content. GA type of rs41919985 in fatty acid synthase (FASN) was significantly associated with the highest amount of C20:5 n-3 (p = 0.031). CC type of rs41729173 in fatty acid-binding protein 4 (FABP4) was significantly associated with the lowest amount of C22:2n-6 (p = 0.047). AG type of rs42187261 in FADS1 was significantly linked to the lowest concentration of C20:4 n-6 (p = 0.044). The total n-6/n-3 ratio of the steer which has all four SNP types in above loci (27.905) was much lower than the mean value of the total n-6/n-3 ratio in phospholipid of the 55 Hanwoo steers ($56.059{\pm}16.180$). It was found that phospholipid and neutral lipid of Hanwoo have very high n-6/n-3 ratios compared to the reported data from different cow breeds. Four SNPs in genes related with fatty acid metabolism showed significant associations with the fatty acid profile of phospholipid and may have potential as SNP markers to select Hanwoo steers in terms of n-6/n-3 balance in the future.

키워드

참고문헌

  1. Simopoulos AP. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother. 2002;56:365-79. https://doi.org/10.1016/S0753-3322(02)00253-6
  2. Daley CA, Abbott A, Doyle PS, Nader GA, Larson S. A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef. Nutr J. 2010;9:10. https://doi.org/10.1186/1475-2891-9-10
  3. Weill P, Schmitt B, Chesneau G, Daniel N, Safraou F, Legrand P. Effects of introducing linseed in livestock diet on blood fatty acid composition of consumers of animal products. Ann Nutr Metab. 2002;46:182-91. https://doi.org/10.1159/000065405
  4. Bourre JM. Where to find omega-3 fatty acids and how feeding animals with diet enriched in omega-3 fatty acids to increase nutritional value of derived products for human: what is actually useful? J Nutr Health Aging. 2005;9:232-42.
  5. Buchanan JW, Reecy JM, Garrick DJ, Duan Q, Beitz DC, Mateescu RG. Genetic parameters and genetic correlations among triacylglycerol and phospholipid fractions in Angus cattle. J Anim Sci. 2015;93:522-8. https://doi.org/10.2527/jas.2014-8418
  6. Dujkova R, Ranganathan Y, Dufek A, Macak J, Bezdicek J. Polymorphic effects of FABP4 and SCD genes on intramuscular fatty acid profiles in longissimus muscle from two cattle breeds. Acta Vet Brno. 2015;84:327-36. https://doi.org/10.2754/avb201584040327
  7. Li C, Aldai N, Vinsky M, Dugan ME, McAllister TA. Association analyses of single nucleotide polymorphisms in bovine stearoyl-CoA desaturase and fatty acid synthase genes with fatty acid composition in commercial cross-bred beef steers. Anim Genet. 2012;43:93-7. https://doi.org/10.1111/j.1365-2052.2011.02217.x
  8. Oh D, Lee Y, La B, Yeo J, Chung E, Kim Y, et al. Fatty acid composition of beef is associated with exonic nucleotide variants of the gene encoding FASN. Mol Biol Rep. 2012;39:4083-90. https://doi.org/10.1007/s11033-011-1190-7
  9. Taniguchi M, Mannen H, Oyama K, Shimakura Y, Oka A, Watanabe H, et al. Differences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle. Livest Prod Sci. 2004;87:215-20. https://doi.org/10.1016/j.livprodsci.2003.07.008
  10. Ibeagha-Awemu EM, Akwanji KA, Beaudoin F, Zhao X. Associations between variants of FADS genes and omega-3 and omega-6 milk fatty acids of Canadian Holstein cows. BMC Genet. 2014;15:25. https://doi.org/10.1186/1471-2156-15-25
  11. Raes K, De Smet S, Demeyer D. Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Anim Feed Sci Technol. 2004;113:199-221. https://doi.org/10.1016/j.anifeedsci.2003.09.001
  12. Folch J, Lees M, Solane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1956;226:497-509.
  13. Tanamati A, Oliveira CC, Visentainer JV, Matsushita M, de Souza NE. Comparative study of total lipids in beef using chlorinated solvent and low-toxicity solvent methods. J Am Oil Chem Soc. 2005;82:393-7. https://doi.org/10.1007/s11746-005-1083-4
  14. Toschi TG, Bendini A, Ricci A, Lercker G. Pressurized solvent extraction of total lipids in poultry meat. Food Chem. 2003;83:551-5. https://doi.org/10.1016/S0308-8146(03)00152-3
  15. O’Fallon JV, Busboom JR, Nelson ML, Gaskins CT. A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J Anim Sci. 2007;85:1511-21. https://doi.org/10.2527/jas.2006-491
  16. Ghatak S, Muthukumaran RB, Nachimuthu SK. A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis. J Biomol Tech. 2013;24:224-31.
  17. National Center for Biotechnology Information. US National Library of Medicine, Rockville Pike. https://blast.ncbi.nlm.nih.gov/Blast.cgi. Accessed 20 Nov 2018.
  18. Vincze T, Posfai J, Roberts RJ. NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res. 2003:31:3688-91. https://doi.org/10.1093/nar/gkg526
  19. Mannen H. Identification and utilization of genes associated with beef qualities. Anim Sci J. 2011;82:1-7. https://doi.org/10.1111/j.1740-0929.2010.00845.x
  20. Guillou H, Zadravec D, Martin PGP, Jacobsson A. The key roles of elongases and desaturases in mammalian fatty acid metabolism: Insights from transgenic mice. Prog Lipid Res. 2010;49:186-99. https://doi.org/10.1016/j.plipres.2009.12.002
  21. Bhuiyan MSA, Yu SL, Jeon JT, Yoon D, Cho YM, Park EW, et al. DNA polymorphisms in SREBF1 and FASN genes affect fatty acid composition in Korean cattle (Hanwoo). Asian-Australas J Anim Sci. 2009;22:765-73. https://doi.org/10.5713/ajas.2009.80573
  22. Hirschhorn JN, Daly MJ. Genome-wide association studies for common diseases and complex traits. Nat Rev Genet. 2005;6:95-108. https://doi.org/10.1038/nrg1521
  23. Oh DY, Jin MH, Lee YS, Ha JJ, Kim BK, Yeo JS, et al. Identification of stearoyl-CoA desaturase (SCD) gene interactions in Korean native cattle based on the multifactor-dimensionality reduction method. Asian-Australas J Anim Sci. 2013;26:1218-28. https://doi.org/10.5713/ajas.2013.13058
  24. Calder PC. Omega-3 polyunsaturated fatty acids and inflammatory processes: nutrition or pharmacology? Br J Clin Pharmacol. 2013;75:645-62. https://doi.org/10.1111/j.1365-2125.2012.04374.x
  25. Harika RK, Eilander A, Alssema M, Osendarp SJM, Zock PL. Intake of fatty acids in general populations worldwide does not meet dietary recommendations to prevent coronary heart disease: a systematic review of data from 40 countries. Ann Nutr Metab. 2013;63:229-38. https://doi.org/10.1159/000355437
  26. Hulbert AJ, Turner N, Storlien LH, Else PL. Dietary fats and membrane function: implications for metabolism and disease. Biol Rev Camb Philos Soc. 2005;80:155-69. https://doi.org/10.1017/S1464793104006578
  27. Kris-Etherton PM, Taylor DS, Yu-Poth S, Huth P, Moriarty K, Fishell V, et al. Polyunsaturated fatty acids in the food chain in the United States. Am J Clin Nutr. 2000;71:179S-88S. https://doi.org/10.1093/ajcn/71.1.179S
  28. Roy R, Taourit S, Zaragoza P, Eggen A, Rodellar C. Genomic structure and alternative transcript of bovine fatty acid synthase gene (FASN): comparative analysis of the FASN gene between monogastric and ruminant species. Cytogenet Genome Res. 2005;111:65-73. https://doi.org/10.1159/000085672
  29. Scollan ND, Choi NJ, Kurt E, Fisher AV, Enser M, Wood JD. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. Br J Nutr. 2001;85:115-24. https://doi.org/10.1079/BJN2000223
  30. Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med (Maywood). 2008;233:674-88. https://doi.org/10.3181/0711-MR-311
  31. Simopoulos AP. Importance of the omega-6/omega-3 balance in health and disease: evolutionary aspects of diet. World Rev Nutr Diet. 2011;102:10-21. https://doi.org/10.1159/000327785
  32. Sugano M, Hirahara F. Polyunsaturated fatty acids in the food chain in Japan. Am J Clin Nutr. 2000;71:189S-96S. https://doi.org/10.1093/ajcn/71.1.189S
  33. Zhang S, Knight TJ, Reecy JM, Beitz DC. DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim Genet. 2008;39:62-70. https://doi.org/10.1111/j.1365-2052.2007.01681.x

피인용 문헌

  1. The effect of polymorphism in the FADS2 gene on the fatty acid composition of bovine milk vol.62, pp.2, 2019, https://doi.org/10.5194/aab-62-547-2019
  2. Erratum to: Study on the fatty acid profile of phospholipid and neutral lipid in Hanwoo beef and their relationship to genetic variation vol.61, pp.6, 2019, https://doi.org/10.5187/jast.2019.61.6.388
  3. Proteome alterations associated with the oleic acid and cis-9, trans-11 conjugated linoleic acid content in bovine skeletal muscle vol.222, 2019, https://doi.org/10.1016/j.jprot.2020.103792
  4. Near-Infrared Reflectance Spectroscopy for Predicting the Phospholipid Fraction and the Total Fatty Acid Composition of Freeze-Dried Beef vol.21, pp.12, 2021, https://doi.org/10.3390/s21124230