한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제27권2호
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  • Pages.143-150
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  • 2019
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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각질형성세포에서 MMP-1 활성 및 자외선 유도 무모쥐 피부손상에 대한 카테킨 고함유 녹차추출물의 영향

Effects of Catechin-rich Green Tea Extract on the MMP-1 Activity of HaCaT Keratinocyte Cells and on UVB-induced Skin Damage in Hairless Mice

  • 양원경 (대전대학교 폐계내과학교실) ;
  • 박양춘 (대전대학교 폐계내과학교실) ;
  • 김복규 (대전대학교 동서생명과학연구원) ;
  • 최정준 (대전대학교 동서생명과학연구원) ;
  • 류건식 ((주) 알앤오식품) ;
  • 김승형 (대전대학교 동서생명과학연구원)
  • Yang, Won Kyung (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Park, Yang Chun (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Kim, Bok Kyu (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Choi, Jeong June (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Ryu, Geon Seek (AlnO Food Inc.(LTD)) ;
  • Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
  • 투고 : 2019.02.08
  • 심사 : 2019.04.16
  • 발행 : 2019.04.30
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초록

Background: Skin is an organ that protects the human body from various environmental stimuli that can induce immune system activation. Skin aging can be largely divided into two categories: physiological aging, which is caused by the a decreased physiological function of the skin and structural changes with aging, and photoaging, which is caused by the chemical stress induced by external stimuli such as ultraviolet (UV) radiation. Methods and Results: The objective of this study was to investigate the anti-wrinkle and UV protective effect of catechin-rich green tea extract (CGTE) in activated keratinocyte (HaCaT cells) and UV-induced skin damage in hairless mice. The results showed that CGTE inhibits the tumor necrosis factor-alpha interferon-gamma ($TNF-{\alpha}+IFN-{\gamma}$)-induced expression of matrix metalloproteinase (MMP)-1 in HaCaT cells. In addition, the CGTE treatment significantly reduced wrinkle formation, epidermal thickness, collagen deposition, and transepidermal water loss in dorsal skin irradiated with UVB. However, the ${\beta}$-glucosidase activity was significantly increased. The CGTE treatment inhibits mRNA expression and enzyme activity of MMP-2 and MMP-9 in the dorsal skin irradiated with UVB. Conclusions: It is expected that CGTE can be effectively used as a functional food and cosmetic ingredient to improve skin moisture retention and reduce wrinkle formation.

키워드

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Fig. 1. HPLC chromatogram of A) standard 5 catechins and caffeine in green tea and of B) whole extract of green tea and C) CGTE.

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Fig. 2. Effect of CGTE on cell viability and MMP-1 expression by TNF-α + IFN-γ treatment in HaCaT cells. HaCaT cells were incubated with TNF-α + IFN-γ (10 ng/㎖) in CGTE (10, 50, 100, 200 and 400 ㎍/㎖) for 24 h.

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Fig. 3. Effect of CGTE on UVB-induced skin wrinkles, epidermal thickening, and histological changes.

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Fig. 4. Effect of CGTE on the UVB-induced skin barrier function perturbation. (A) TEWL and (B) β- glucosidase was measured as a marker of the skin barrier function.

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Fig. 5. Effect of CGTE on MMP-2 and MMP-9 mRNA expression in animal skin exposed to UVB.

Table 1. HPLC running condition for tea catechins and extract.

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Table 2. The primer sequence used for quantitative real-time PCR.

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