Fig. 3. A phylogenetic tree performed by the neighbor-joining method showing the relationship of Bacillus aryabhattai H26-2 and Bacillus siamensis H30-3 with other species of the genus Bacillus based on 16S rRNA sequences analysis. Numbers at the blanching points are the bootstrap values (> 50%) for 1,000 replicates. A scale bar indicates 5 nucleotide substitutions per 1000 nucleotides of the sequence. Strains H26-2 and H30-3 are shown in bold type. T, type strain.
Fig. 1. (A) Growth promotion by Bacillus aryabhattai H26-2 and Bacillus siamensis H30-3 compared to 10 mM MgSO4 solution (control) in Chinese cabbage (‘Ryeokkwang’ and ‘Buram-3-ho’) under non-stress condition; (B) Leaf wilting score (0-5) under heat and drought conditions; (C) fresh weight after rewatering of Chinese cabbages (‘Ryeokkwang’ and ‘Buram-3-ho’). Plants were treated with bacterial strains H26-2 and H30-3, or 10 mM MgSO4 solution (untreated control), one week later, heat and drought stresses were treated; Twenty-four h after end of stress treatment, fresh weight were assessed. Error bars indicate the standard errors of 20 replications from two experiments. An asterisk on bars with standard errors indicate significant (P < 0.05) differences between treatments according to the least significant difference test.
Fig. 2. (A) Abscisic acid (ABA) contents and (B) stomatal opening (%) in leaves of Chinese cabbages (‘Ryeokkwang’ and ‘Buram-3-ho’) at 24 h after heat and drought stresses treatment. Plants were drenched with strain H26-2 and H30-3, and 10mM MgSO4 solution as untreated control, one week later, heat and drought stresses were performed. Error bars indicate the standard errors of eight (for ABA contents) or 16 (for stomatal opening) replications from two experiments. An asterisk on bars with error bars indicate significant (P < 0.05) differences between treatments according to the least significant difference test.
Table 1. Bacterial colonization in rhizosphere soils of Chinese cabbages treated with spontaneous rifampicin-resistant mutants, H26-2 Rif and H30-3 Rif under normal and stressed conditions
Table 2. Effect of exopolysaccharides of H26-2 and H30-3 on cotyledon greening (%) and number of lateral roots after germination in plate assay, and fresh weight and leaf wilting score in Chinese cabbage under non-stressed or heat and drought stressed conditions
Table 3. Biocontrol activity of tested bacterial strains against soft rot caused by Pectobacterium carotovorum subsp. carotovorum PCC21 in two cultivars of Chinese cabbages
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