Asian-Australasian Journal of Animal Sciences

  • 제32권6호
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  • Pages.856-864
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  • 2019
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Mixed organic acids improve nutrients digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high-fiber diet

  • Li, Miao (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Long, Shenfei (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Wang, Qianqian (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Zhang, Lianhua (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Hu, Jiangxu (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Yang, Jie (Yunnan Kuaidaduo Animal Husbandry Technology Co., LTD) ;
  • Cheng, Zhibin (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Piao, Xiangshu (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University)
  • 투고 : 2018.07.07
  • 심사 : 2018.10.25
  • 발행 : 2019.06.01
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초록

Objective: The objective of this study was to investigate effects of mixed organic acids (MOA) on nutrient digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high wheat bran diet. Methods: Six crossbred barrows ($Duroc{\times}Landrace{\times}Yorkshire$), with an average body weight $78.8{\pm}4.21kg$, fitted with T-cannulas at the distal ileum, were allotted to a double $3{\times}3$ Latin square design with 3 periods and 3 diets. Each period consisted of a 5-d adjustment period followed by a 2-d total collection of feces and then a 2-d collection of ileal digesta. The dietary treatments included a corn-soybean-wheat bran basal diet (CTR), mixed organic acid 1 diet (MOA1; CTR+3,000 mg/kg OA1), mixed organic acid 2 diet (MOA2; CTR+2,000 mg/kg OA2). Results: Pigs fed MOA (MOA1 or MOA2) showed improved (p<0.05) apparent total tract digestibility (ATTD) of gross energy, dry matter and organic matter, and pigs fed MOA2 had increased (p<0.05) ATTD of neutral detergent fiber compared to CTR. Dietary MOA supplementation decreased (p<0.05) pH value, and improved (p<0.01) concentrations of lactic acid and total volatile fatty acids (TVFA) in ileum compared to CTR. Pigs fed MOA showed higher (p<0.05) concentration of acetic acid, and lower (p<0.05) content of formic acid in feces compared to CTR. Pigs fed MOA1 had increased (p<0.05) concentration of TVFA and butyric acid in feces. Pigs fed MOA1 showed higher concentration of Lactobacillus and lower concentration of Escherichia in feces compared to CTR. Conclusion: Dietary supplementation of MOA 1 or 2 could improve nutrients digestibility, TVFA concentration and intestinal flora in growing-finishing pigs fed high fiber diet.

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참고문헌

  1. Saleem AM, Zanouny AI, Singer AM. Growth performance, nutrients digestibility, and blood metabolites of lambs fed diets supplemented with probiotics during pre- and post-weaning period. Asian-Australas J Anim Sci 2017;30:523-30. https://doi.org/10.5713/ajas.16.0691
  2. Li JY. Current status and prospects for in-feed antibiotics in the different stages of pork production - a review. Asian-Australas J Anim Sci 2017;30:1667-73. https://doi.org/10.5713/ajas.17.0418
  3. Yousaf MS, Goodarzi Boroojeni F, Vahjen W, Zentek J. Encapsulated benzoic acid supplementation in broiler diets influences gut bacterial composition and activity. Br Poult Sci 2017;58:122-31. https://doi.org/10.1080/00071668.2016.1262000
  4. Luise D, Motta V, Salvarani C, et al. Long-term administration of formic acid to weaners: influence on intestinal microbiota, immunity parameters and growth performance. Anim Feed Sci Technol 2017;232:160-8. https://doi.org/10.1016/j.anifeedsci.2017.06.015
  5. Upadhaya SD, Lee KY, Kim IH. Protected organic acid blends as an alternative to antibiotics in finishing pigs. Asian-Australas J Anim Sci 2014;27:1600-7. https://doi.org/10.5713/ajas.2014.14356
  6. Hanczakowska E, Szewczyk A, Swiatkiewicz M, Okon K. Shortand medium-chain fatty acids as a feed supplement for weaning and nursery pigs. Pol J Vet Sci 2013;16:647-54. https://doi.org/10.2478/pjvs-2013-0092
  7. Partanen KH, Mroz Z. Organic acids for performance enhancement in pig diets. Nutr Res Rev 1999;12:117-45. https://doi.org/10.1079/095442299108728884
  8. Partanen K, Jalava T, Valaja J. Effects of a dietary organic acid mixture and of dietary fibre levels on ileal and faecal nutrient apparent digestibility, bacterial nitrogen flow, microbial metabolite concentrations and rate of passage in the digestive tract of pigs. Animal 2007;1:389-401. https://doi.org/10.1017/S1751731107657838
  9. Lueck E, Jager M. Antimicrobial food additives: characteristics, uses, effects. Germany: Springer-Verlag; 1980.
  10. Ahmed ST, Hwang JA, Hoon J, Mun HS, Yang CJ. Comparison of single and blend acidifiers as alternative to antibiotics on growth performance, fecal microflora, and humoral immunity in weaned piglets. Asian-Australas J Anim Sci 2014;27:93-100. https://doi.org/10.5713/ajas.2013.13411
  11. Zentek J, Ferrara F, Pieper R, Tedin L, Meyer W, Vahjen W. Effects of dietary combinations of organic acids and medium chain fatty acids on the gastrointestinal microbial ecology and bacterial metabolites in the digestive tract of weaning piglets. J Anim Sci 2013;91:3200-10. https://doi.org/10.2527/jas.2012-5673
  12. Xu YT, Liu L, Long SF, Pan L, Piao XS. Effect of organic acids and essential oils on performance, intestinal health and digestive enzyme activities of weaned pigs. Anim Feed Sci Technol 2018; 235:110-9. https://doi.org/10.1016/j.anifeedsci.2017.10.012
  13. Long SF, Xu YT, Pan L, et al. Mixed organic acids as antibiotic substitutes improve performance, serum immunity, intestinal morphology and microbiota for weaned piglets. Anim Feed Sci Technol 2018;235:23-32. https://doi.org/10.1016/j.anifeedsci.2017.08.018
  14. Zhao JB, Zhang S, Xie F, Li DF, Huang, C. Effects of inclusion level and adaptation period on nutrient digestibility and digestible energy of wheat bran in growing-finishing pigs. Asian-Australas J Anim Sci 2018;31:116-22. https://doi.org/10.5713/ajas.17.0277
  15. Shaw DT, Rozeboom DW, Hill GM, et al. Impact of vitamin and mineral supplement withdrawal and wheat middling inclusion on finishing pig growth performance, fecal mineral concentration, carcass characteristics, and the nutrient content and oxidative stability of pork. J Anim Sci 2002;80:2920-30. https://doi.org/10.2527/2002.80112920x
  16. Caspar Wenk. The role of dietary fibre in the digestive physiology of the pig. Anim Feed Sci Technol 2001;90:21-33. https://doi.org/10.1016/S0377-8401(01)00194-8
  17. Hashemipour H, Khaksar V, Rubio LA, Veldkamp T, van Krimpen MM. Effect of feed supplementation with a thymol plus carvacrol mixture, in combination or not with an NSP-degrading enzyme, on productive and physiological parameters of broilers fed on wheat-based diets. Anim Feed Sci Technol 2016;211:117-31. https://doi.org/10.1016/j.anifeedsci.2015.09.023
  18. Stein HH, Shipley CF, Easter RA. Technical note: a technique for inserting a T-cannula into the distal ileum of pregnant sows. J Anim Sci 1998;76:1433-6. https://doi.org/10.2527/1998.7651433x
  19. NRC. Nutrient requirements of swine, eleventh revised editon. Washington, DC, USA: National Academy Press; 2012.
  20. Zeng ZK, Piao XS, Wang D, et al. Effect of microbial phytase on performance, nutrient absorption and excretion in weaned pigs and apparent ileal nutrient digestibility in growing pigs. Asian-Australas J Anim Sci 2011;24:1164-72. https://doi.org/10.5713/ajas.2011.11016
  21. AOAC. Official methods of analysis. 16th edition. Association of Official Analytical Chemists. Washington, DC, USA: AOAC International; 1995.
  22. Williams CH, David DJ, Iismaa O. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. J Agric Sci 1962;59:381-5. https://doi.org/10.1017/S002185960001546X
  23. Edgar, RC. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat Methods 2013;10:996-8. https://doi.org/10.1038/nmeth.2604
  24. Segata N, Izard J, Waldron L, Miropolsky L, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol 2011;12:R60. https://doi.org/10.1186/gb-2011-12-6-r60
  25. Tonel I, Pinho M, Lordelo MM, Cunha LF, Garres P, Freire JPB. Effect of butyrate on gut development and intestinal mucosa morphology of piglets. Livest Sci 2010;133:222-4. https://doi.org/10.1016/j.livsci.2010.06.069
  26. Gerritsen R, Van Dijk AJ, Rethy K, Bikker P. The effect of blends of organic acids on apparent faecal digestibility in piglets. Livest Sci 2010;134:246-8. https://doi.org/10.1016/j.livsci.2010.06.154
  27. Partanen K, Siljander-Rasi H, Suomi K. Dietary preferences of weaned piglets offered diets containing organic acids. Agric Food Sci Finland 2002;11:107-19. https://doi.org/10.23986/afsci.5718
  28. Canani RB, Di Costanzo M, Leone L. The epigenetic effects of butyrate: potential therapeutic implications for clinical practice. Clin Epigenetics 2012;4:4. https://doi.org/10.1186/1868-7083-4-4
  29. Traul KA, Driedger A, Ingle DL, Nakhasi D. Review of the toxicologic properties of medium-chain triglycerides. Food Chem Toxicol 2000;38:79-98. https://doi.org/10.1016/S0278-6915(99)00106-4
  30. Richards JD, Gong J, de Lange CFM. The gastrointestinal microbiota and its role in monogastric nutrition and health with an emphasis on pigs: current understanding, possible modulations, and new technologies for ecological studies. Can J Anim Sci 2005;85:421-35. https://doi.org/10.4141/A05-049
  31. Overland M, Kjos NP, Borg M, Skjerve E, Sorum H. Organic acids in diets for entire male pigs: Effect on skatole level, microbiota in digesta, and growth performance. Livest Sci 2008;115: 169-78. https://doi.org/10.1016/j.livsci.2007.07.007
  32. Kluge H, Broz J, Eder K. Effect of benzoic acid on growth performance, nutrient digestibility, nitrogen balance, gastrointestinal microflora and parameters of microbial metabolism in piglets. J Anim Physiol Anim Nutr 2006;90:316-24. https://doi.org/10.1111/j.1439-0396.2005.00604.x
  33. Koopmans SJ, Bikker P, van Wikselaar PG, van Krimpen MM. Inoculated fermented corn as dietary performance and health enhancer in pigs: literature and in vitro study. Wageningen, The Netherlands: Wageningen UR (University & Research Centre); 2016. Livestock Research 955. pp. 3-19.
  34. Ley RE, Turnbaugh P, Klein S, Gordon JI. Microbial ecology: human gut microbes associated with obesity. Nature 2006;444: 1022-3. https://doi.org/10.1038/4441022a
  35. Guilloteau P, Martin L, Eeckhaut V, Ducatelle R, Zabielski R, Van Immerseel F. From the gut to the peripheral tissues: the multiple effects of butyrate. Nutr Res Rev 2010;23:366-84. https://doi.org/10.1017/S0954422410000247

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