Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Complete genome sequence of Marinobacter salarius HL2708#2 isolated from a lava sea water environment on Jeju Island

제주용암 해수 환경에서 분리한 Marinobacter salarius HL2708#2의 유전체 해독

  • Received : 2019.01.02
  • Accepted : 2019.01.16
  • Published : 2019.03.31
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Abstract

During screening of microbes for compounds having cosmetic benefits, we isolated Marinobacter salarius HL2708#2 from lava seawater on Jeju Island, Republic of Korea. The complete genome sequence was determined. Strain HL27080#2 features a circular chromosome of 4,304,603 bp with 57.21% G+C content and a 244,163 bp plasmid with 53.14% G+C. There were 4,180 protein coding sequences identified, along with 49 transfer RNA and 18 ribosomal RNA noncoding genes. The genome harbored genes for the utilization of alcohol, maltose/starch, and monosaccharide as sole carbon sources. Genes responsible for halophilic characteristics and heavy metal resistance could be annotated, as well as aromatic and alkane hydrocarbons. Contrary to the prior report that M. salarius is negative for nitrate and nitrite reduction, nitrate/nitrite reductase along with nitrate/nitrate transporters and nitronate monooxygenase were evident, suggesting that strain HL2708#2 may be able to denitrify extracellular nitroalkenes to ammonia.

향장원료 개선을 위한 미생물 탐색 실험을 통하여 Marinobacter salarius HL2708#2을 제주도의 용암 해수 환경에서 분리하였다. 균주 HL278#2의 완전한 게놈 서열을 분석하였으며, 원형 염색체는 4,304,603 bp이고 57.21% G+C이고 플라스미드는 244,163 bp이고 53.14% G+C였다. 4,180개의 단백질 코딩서열이 과 49개의 tRNA와 18개의 rRNA 유전자와 함께 확인되었다. 균주 HL2708# 2의 게놈은 알콜, 말토덱스트린/전분 및 단당류 대사 유전자를 보유하고 있었다. 호염성 및 중금속 저항성을 담당하는 유전자와 방향족 및 알칸 계열 탄화수소를 대사하는 유전자를 가지고 있는 것으로 분석되었다. Marinobacter salarius가 질산염 및 아질산염 환원능력이 없다고 알려져 있는 것과 달리, HL2708#2 균주는 질산염/아질산염 환원 효소, 질산염/질산염 운반체 및 질산염 모노 옥시게나제를 가지고 있는 것으로 보아 세포 체외의 니트로알켄을 활용할 수 있는 능력을 가진 것으로 사료된다.

Keywords

MSMHBQ_2019_v55n1_69_f0001.png 이미지

Fig. 1. Graphical circular map of the chromosome (A) the pHL2708Z5 (B) of HL2708#2.

Table 1. General feature of genome

MSMHBQ_2019_v55n1_69_t0001.png 이미지

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