KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Characterization of Heading- and Yield-related Gene Loci in the Cheongcheong/Nagdong Doubled Haploid Line using Rice QTLs

청청/낙동 배가반수체 집단에서 QTL을 통한 출수기와 수량관련 유전자좌 분석

  • Jang, Yoon-Hee (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Park, Jae-Ryoung (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Kyung-Min (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • 장윤희 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 박재령 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김경민 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2019.01.30
  • Accepted : 2019.03.20
  • Published : 2019.03.31
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Abstract

A quantitative trait loci (QTL) analysis of traits related to heading and yield was performed develop rice cultivars that are both early maturing and panicle weight type varieties. Our analysis included 120 strains of the Cheongcheong Nagdong doubled haploid (CNDH) variety. An observational growth experiment was conducted to identify genetic agronomic traits of CNDH. Heading date, ten plant weight, moisture, thousand grain weight, and yield had a normal distribution based on the frequency distribution table of the observational growth data. The QTL analysis found one heading-related and nine yield-related QTLs. The LOD of 2.85 was the largest in QTLs for heading date (QHD), 5.39 in QTLs for ten plant weight (QTPW), 3.92 in QTLs for moisture (QM), 4.80 in QTLs for thousand grain weight (QTGW), and 3.7 in QTLs for yield (QY). Genomic analysis detected 58 candidate genes on chromosome 2, 3, 7, 8, and 10. Among those, we found Rcd1 protein and OsERF3 gene in QM, MtN3 and zinc finger protein genes in QTGW, and OsNAC3 protein gene in QY. If further analysis reveals the presence of genes related to water content, thousand grain weight or yield in the CNDH stains, we can develop a selection of varieties that will be capable of coping with climate change and will contribute to global food problems.

본 연구는 2017년 CNDH 계통을 이용하여 출수기와 수량을 QTL 조사하여 다음과 같은 결과를 가졌다. 1. 도수분포표에서 QHD, QTPW, QM, QTGW, QY는 정규분포를 이루고 있었다. 2. 출수기 관련 QTLs은 총 1개가 잡혔고, 수량관련 QTLs은 총 9개가 잡혔다. QHD에서는 LOD 2.85가 가장 컸고, QTPW에서는 5.39, QM에서는 3.92, QTGW에서는 4.80, QY에서는 3.7이 가장 컸다. 3. 유전자 분석을 통해, 2, 3, 7, 8, 10번 염색체에서 총58개의 후보유전자를 찾았다. 이 중 수량요소인 QM에서 Rcd1 protein, OsERF3 유전자, QTGW에서 MtN3, Zinc finger protein 유전자, QY에서 OsNAC3 protein 유전자를 발견하였다. 4. 본 연구를 통해 발견된 CNDH 계통 내의 수분함량, 천립중, 수량에 관련된 유전자의 존재여부를 찾아낸다면 조생종, 수중형에 가까운 품종을 개발하는 데 기초자료로 이용될 수 있을 것이라 판단된다.

Keywords

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Fig. 1. Genealogical chart of CNDH.

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Fig. 2. The frequency distributions for growth survey in CNDH lines. The black arrow represents Cheongcheong and the white arrow represents Nagdong. (a) Culm length. Cheongcheong : 62.9 cm, Nagdong : 44.6 cm. (b) Panicle length. Cheongcheong : 19.2 cm, Nagdong : 16.1 cm. (c) The number of panicles. Cheongcheong : 10.1, Nagdong : 7.8. (d) 1000 grain weight in CNDH lines. Cheongcheong : 22.0 g, Nagdong : 19.8 g. (e) Days to heading. Cheongcheong : 107.1 days, Nagdong : 107.8 days. (f) Ten plants weight. Cheongcheong : 99.8 g, Nagdong : 54.0 g. (g) Moisture. Cheongcheong : 12.3%, Nagdong : 14.6%. (h) Yield. Cheongcheong : 327.8 kg, Nagdong : 115.8 kg.

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Fig. 3. The QTL analysis results were associated with rice traits in CNDH lines. (a): QTL of heading date appeared on chromosome 8. The LOD value was 2.85. (b): QTL of 10 plants weight appeared on chromosome 2. The LOD value was 5.39. (c): QTL of moisture appeared on chromosome 2. There were two QTL analysis results. The LOD values were 3.08 and 3.92, respectively. (d): QTL of moisture appeared on chromosome 7. The LOD value was 3.0. (e): QTL of 1000 grain weight appeared on chromosome 2. The LOD value was 4.80. (f): QTL of 1000 grain weight appeared on chromosome 3. The LOD value was 3.22. (g): QTL of 1000 grain weight appeared on chromosome 10. There were two QTL analysis results. The LOD values were 3.4 and 3.09, respectively. (h): QTL of yield appeared on chromosome 10. The LOD value was 3.7.

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Fig. 4. The chromosomal location of QTLs associated with rice traits in CNDH lines. (a): QTL of heading date appeared on chromosome 8. The region extended from RM404 to RM22861. (b): QTL of 10 plants weight appeared on chromosome 2. The region extended from RM13021 to RM1106. (c): QTL of moisture appeared on chromosome 2. The first region extended from RM1106 to RM424. The second region extended from RM13713. (d): QTL of moisture appeared on chromosome 7. The region extended from RM1134 to RM8261. (e): QTL of 1000 grain weight appeared on chromosome 2. The region extended from RM1211. (f): QTL of 1000 grain weight appeared on chromosome 3. The region extended from RM15927. (g): QTL of 1000 grain weight appeared on chromosome 10. The first region extended from RM25219. The second region extended from RM25181. (h): QTL of yield appeared on chromosome 10. The region extended from RM6776.

Table 1. The plant traits of 120 DH lines derived from a cross of Cheongcheong and Nagdong.

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Table 2. QTL information on QHD, QTPW, QM, QTGW, QY.

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