Fig. 1. HPLC chromatogram of extract of soybean germ. (A) Daidzin (B) Genistin (C) Daidzein (D) Genistein
Fig. 2. Amount of isoflavone isolated from Soybean germ.
Fig. 3. Bioconversioin yield of isoflavones extracted from soybean germ using Pectinase.
Fig. 4. Effect of bioconversioned soybean germ extract on cell viability. Result is resented as mean ± SD. (A) RAW 264.7 cells (B) B16F10 cells (C) HDF cells.
Fig. 5. Inhibitory effect of bioconversioned soybean germ extract on LPS-induced NO production by RAW 264.7 cells. Result is represented as mean ± SD. *p<0.05
Fig. 6. Inhibition of melanin synthesis in B16F10 melanoma cells treated with bioconversioned soybean germ extract. Result is represented as mean ± SD. *p<0.05 **p<0.01 ***p<0.001
Fig. 7. Inhibitory effects of bioconversioned soybean germ extract on UVB-induced MMP-1 in HDF cells. Result is represented as mean ± SD. **p<0.01 ***p<0.001
Table 1. Gradient condition for HPLC analysis
Table 2. Formulation of wash-off pack containing bioconversioned soybean germ extract
Table 3. Irritant classification criteria and skin irritation score system (A) Irritant classification criteria (B) skin rritation score systemA
Table 4. Changes in roughness of skin.
Table 5. Changes in roughness of skin.
References
- J. H. Son, M. G. Choung, H. J. Choi, U. B. Jang, G. M. Son, M. W. Byun, C. Choi. (2001). Physiological Effect of Korean Black Soybean Pigment. Korean Journal of Food Science and Technology. 33(6), 764-768.
- C. H. Kim, J. S. Park, H. S. Sphn, C. W. Chung. (2002). Determination of Isoflavone, Total Saponin, Dietary Fiber, Soy Oligosaccharides and Lecithins from Commercial Soy Products Based on the One Serving Size - Some bioactive compounds from commercialized soy products. Korean Journal of Food Science and Technology. 34(1), 96-102.
-
J. H. Shin, N. M. Joo. (2016). Component Changes in Antioxidant Activity and Isoflavones (
${\beta}$ -glucoside & aglycone) Contents of Small Black Bean According to Different Cooking Methods. Korean Journal of Food & Cookery Science, 32(2), 10. DOI : 9724/kfcs.2016.32.2.197 - H. Y. Park, E. J. Cho. (2008). Radical Scavenging Effects and Physicochemical Properties of Seolitae Chungkukjang Added with Green Tea. Journal of the Korean Society of Food Science and Nutrition, 37(4), 401-404. DOI : 10.3746/jkfn.2008.37.4.401
- H. Y. Cho, J. L. Yang, K. H. Noh, J. J. Kim, Y. H. Kim, K. H. Huh, Y. S. Song. (2007). Anti-atherogenic Effect of Isoflavone through Hypolipidemic, Anti-oxidative and Anti-inflammatory Actions in C57BL/6 Mice. Korean society of food and nutrition, 36(3), 276-283. DOI : 10.3746/jkfn.2007.36.3.276
- H. G. Kim, M. J. Lee, H. J. Kim, K. C. Kim, Shambhunath Bose. (2012). Effects of Fermented Soybean upon Anti.inflammation and Intestinal Mucous Membrane Permeability. Journal of Society of Korean Medicine for Obesity Research, 12(1), 33-47.
- Xin Zhao, J. H. Ju, K. Y. Park. (2017). Comparisons of Properties, In Vitro Anti-Mutagenicity, and Anti-Cancer Effects of Short-Term Fermented Soybean Foods (Chungkukjang, Shuidouchi, and Natto). The Korean Society of Food Science and Nutrition, 46(10), 1253-1257.
- S. Y. Lim, K. Y. Park, M. S. Bae, K. H. Kim. (2009). Effect of Doenjang with Black Soybean on Cytokine Production and Inhibition of Tumor Metastasis. Journal of Life Science, 19(2), 264-270. DOI :10.5352/jls.2009.19.2.264
- J. H. Lee, Y. S. Park, H. M. Park, J. M. Heo. (2012). Survey on the Consumption of the Phytoestrogen Isoflavone in Postmenopausal Korean Women. Journal of Menopausal Medicine, 18(3), 163-173. DOI : 10.6118/jksm.2012.18.3.163
-
K. W. Kim, B. S. Chun. (2001). Optimum Conversion to the Aglycone Form Using
${\beta}$ -glucosidase and Isoflavone Extraction from Soybean. KSBB Journal, 16(2), 174-178. - D. S. Lee, S. Y. Byun. (2001). Effects of the Dietary Mixture of Eucommia ulmoides Oliver on Osteoporosis. KSBB Journal, 16(6), 614-619.
- B. G. Kim, C. K. Oh, J. H. Jo, J. B. Lee, M. B. Kim, H. S. Jang, K. S. Kwon. (2003). Effects of Estrogens and Isoflavones on the Collagen Synthesis. Korean Journal of Dermatology. 41(8), 1041-1048.
- M. S. Lee, S. H. Li. (2010). Research on the perception of fermented cosmetics and the satisfaction level of 'Korean Medicine Herb' cosmetics. Korean journal of aesthetics and cosmetics society, 8(3), 285-292.
- M. S. Joo, S. H. Li. (2012). The Effects on Middle-aged Women's Skin with Resveratrol Cosmetics and Supplement Intake. Korean journal of aesthetics and cosmetics society, 18(3), 643-650.
- K. H. Park, X. M. Piao, E. K. Jang, Y. E. Yoo, T. Y. Hwang, S. L. Kim, J. H. Jong, H. M. Shin. (2012). Variation of Isoflavone Contents in Korean Soybean Cultivars Released from 1913 to 2006. Korean Journal of Breeding Science, 44(2), 149-159
- A. NAKAMURA, H. FURUTA, H. MAEDA, Y. NAGAMATSU, A. YOSHIMOTO. (2001). Analysis of Structural Components and Molecular Construction of Soybean Soluble Polysaccharides by Stepwise Enzymatic Degradation. Biotechnology and Biochemistry, 65(10), 2249-2258. DOI : 10.1271/bbb.65.2249
- G. Repetto, Ana del Peso, J. L Zurita. (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols, 3, 1125-1131. DOI : 10.1038/nprot.2008.75
- David S Chi, Min Qui, Guha Krishnaswamy, Chuanfu Li, William Stone. (2003). Regulation of nitric oxide production from macrophages by lipopolysaccharide and catecholamines. Nitric Oxide. 8(2). 127-132. DOI : 10.1016/s1089-8603(02)00148-9
- D. S. Kim, Y. M. JEONG, I. K. PARK, H. G. HAHN, H. K. LEE, S. B. KWON, J. H. JEONG, S. J. YANG, U. D. SOHN, K. C. PARK. (2007). A New 2-Imino-1,3-thiazoline Derivative, KHG22394, Inhibits Melanin Synthesis in Mouse B16 Melanoma Cells. Biol. Pharm. Bull. 30(1), 180-183. DOI :10.1248/bpb.30.180
- R. Korhonen, A. Lahti, H. Kankaanranta, E. Moilanen. (2005). Nitric oxide production and signaling in inflammation. Current Drug Targets, 4(4), 471-479. DOI : 10.2174/1568010054526359
-
Hamalainen M1, Nieminen R, Vuorela P, Heinonen M, Moilanen E. (2007). Anti-Inflammatory Effects of Flavonoids: Genistein, Kaempferol, Quercetin, and Daidzein Inhibit STAT-1 and
$NF-{\kappa}B$ Activations, Whereas Flavone, Isorhamnetin, Naringenin, and Pelargonidin Inhibit only$NF-{\kappa}B$ Activation along with Their Inhibitory Effect on iNOS Expression and NO Production in Activated Macrophages. Mediators of Inflammation. DOI : 10.1155/2007/45673 - DENISE J. SCHWAHN, WEIDONG XU, AMEY B. HERRIN, ELISE S. BALES, ESTELA E. MEDRANO. (2002). Tyrosine Levels Regulate the Melanogenic Response to alpha-Melanocyte-Stimulating Hormone in Human Melanocytes: Implications for Pigmentation and Proliferation. Pigment Cell Research.. 14(1), 32-39. DOI : 10.1034/j.1600-0749.2001.140106
- SewonKangJin, HoChung1Joo, HeungLee2Gary, J.FisherYian, ShengWanElizabeth, A.DuellJohn J.Voorhees. (2016). Molecular mechanisms of flavonoids in melanin synthesis and the potential for the prevention and treatment of melanoma. Nutrition and Cancer. 60(6), 1264-1274. DOI : 10.1002/mnfr.201500822
- Kelly K. Dong, Niusha Damaghi, Stephanie D. Picart, Nelli G. Markova, Kei Obayashi, Yuri Okano, Hitoshi Masaki, Susanne Grether-Beck, Jean Krutmann, Kenneth A. Smiles, Daniel B. Yarosh. (2008). UVinduced DNA damage initiates release of MMP-1 in human skin. Experimental Dermatology, 17(12), 1037-1044. DOI : 10.1111/j.1600-0625.2008.00747
- Sewon Kang Jin, Ho Chung Joo, Heung Lee, Gary. J. Fisher Yian, Sheng Wan Elizabeth, A. DuellJohn, J. Voorhees. (2002). Topical N-Acetyl Cysteine and Genistein Prevent Ultraviolet-Light-Induced Signaling That Leads to Photoaging in Human Skin in vivo. Nutrition and Cancer. 60(6), 1264-1274. DOI : 10.1046/j.1523-1747.2003.12122.x
- B. Y. Sin, H. P. Kim. (2005). Inhibition of collagenase by naturally-occurring flavonoids. Archives of Pharmacal Research. 28(10), 1152- 1155. DOI : 10.1007/BF02972978