A Study on the Physicochemical Properties and the Nitrite Scavenging Ability of Japanese Sumac Extracts

붉나무 추출물의 이화학적 특성과 아질산염 소거능에 관한 연구

Abstract

Japanese sumac (Rhus javanica) is one of the common herbaceous plants growing over the country. This study was conducted to investigate the extraction characteristics and physiological activities inluding nitrite scavenging ability of the water extracts from leaf, fruit and bark of Japanease sumac. Extraction yield was 6.62~13.84%, free amino acids were detected as 24 kinds with 37.9 mg/100g in leaf extract, 23 kinds with 27.0 mg/100g in fruit extract and 27 kinds with 39.0 mg/100g in bark extract, respectively, and seven kinds essential amino acids were detected. Total contents of flavonoids equivalent to naringin were 587.2 mg/100g in bark extract, 557.3 mg/100g in fruit extract and 379.9 mg/100g in leaf extract, respectively. Total contents of phenolics equivalent to gallic acid were 111.2 mg/100g in leaf extract, 108.4 mg/100g in fruit extract and 80.4 mg/100g in bark extract, respectively. The nitrite scavenging ability of extracts was order of 61.93% in bark extract>57.38% in fruit extract>55.49% in leaf extract, and was 78.1~100% of those of BHT (butylated hydroxytoluene) equivalents at pH 1.2. The electron donating ability was order of 47.38% in fruit extract>43.06% in leaf extract>38.55% in bark extract, and was compared to 65.6%, 58.8% and 53.6% of those of BHT equivalents, respectively. The reduction power was evaluated to 37% higher in leaf extract, 43% higher in fruit extract and 46% higher in bark extract than those of BHT equivalents. The metal chelating ability of extracts was considerably low and was order of 27.3% in bark extract>20.6% in leaf extract>11.2% in fruit extract.

야산에 자생하는 붉나무(Japanese sumac)의 천연자원으로서의 유용성을 확인하기 위하여 잎, 열매 및 수피 등 세부위를 열수로 추출하고, 이들 추출물의 몇 가지 추출특성과 아질산염 소거능 등 생리활성을 측정하였다. 추출물의 수율은 부위에 따라 6.62~13.84%의 범위였고, 유리 아미노산은 잎, 열매, 수피에서 각각 24종 37.9 mg/100g, 23종 27.0 mg/100g 및 27종 39.0 mg/100g의 함량이었으며, 7종의 필수 아미노산이 잎>수피>열매의 순으로 측정되었다. Naringin 상당량으로 구한 총플라보노이드 함량은 수피>열매>잎의 순으로 379.9~587.2 mg/100g이었으며, gallic acid 상당량으로 구한 총페놀성 화합물 함량은 잎>열매>수피의 순으로 80.4~111.2 mg/100g이었다. 붉나무 추출물의 아질산염 소거능은 pH 1.2에서 수피 61.93%>열매 57.38%>잎 55.49%의 순으로 측정되었으며, BHT (butylated hydroxytoluene) 소거능의 78.1~100%에 대응되었다. 추출물의 전자공여능은 열매 47.38%>잎 43.06%>수피 38.55%로서 각각 BHT의 65.6%, 58.8% 및 53.6%의 활성으로 비교되었다. 붉나무의 잎, 열매 및 수피 추출물의 환원력은 대조구로 사용한 BHT의 환원력에 비하여 각각 37%, 43% 및 46% 더 높게 평가되었으며, 금속이온 봉쇄능은 수피 27.3%>잎 20.6%>열매 11.2%의 순으로 상당히 낮게 측정되었다.

Fig. 1. Experimental procedure of physicochemical and physiological property of japanese sumac extract.

Fig. 2. Nitrite scavenging ability of Japanese Sumac extracts with pH.

Fig. 3. Comparison of nitrite scavenging ability between Japanese Sumac extracts and BHT at pH 1.2.

Fig. 4. Comparison of electron donating ability between Japanese Sumac extracts and BHT by DPPH radical scavenging.

Fig. 5. Comparison of reduction power between Japanese Sumac extracts and BHT.

Fig. 6. Metal ion chelating ability of Japanese Sumac extracts.

Table 1. Physicochemical properties of Japanese Sumac extracts

Table 2. Contents of total aromatics, total flavonoid and total phenolics in Japanese Sumac extracts

Table 3. Contents of free amino acid in Japanese Sumac extracts