Fig. 1. Confirmation of the T-DNA genes on the Bt-transgenic (Bt-9) rice and non-genetically modified rice (Ilmi). M, 100bp DNA ladder; N, non-GM rice (Ilmi); Bt-9, Bt-transgenic rice T6; Bar, Phosphinothricin acetyltransferase; SPS, Sucrose-phosphate synthase.
Fig. 2. Confirmation of genes expression for Bt-transgenic (Bt-9) rice by using immunostrip. (a) immunostrip tests for the PAT (phosphinothricin acetyltransferase) detection, (b) immunostrip tests for the CryIAc1 detection. N, non-genetically modified rice (Ilmi); Bt-9, Bt-transgenic rice T6.
Fig. 3. mCryIAc1 protein levels (μg/g fresh weight) in Ilmi and Bt-transgenic (Bt-9) rice. Values are the average ± SD of triplicate measures.
Table 1. Primers list used for PCR (Polymerase chain reaction) analysis.
Table 2. Changes of water temperature (℃) during cumulative immobility tests of Daphnia magna in non-Genetically modified rice (Ilmi) and Bt-transgenic (Bt-9) rice.
Table 3. Changes of pH during cumulative immobility tests of Daphnia magna in non-genetically modifed rice (Ilmi) and Bt-transgenic (Bt-9) rice.
Table 4. Cumulative immobility of Daphnia magna in non-genetically modifed rice (Ilmi) and Bt-transgenic (Bt-9) rice.
Table 5. EC50 (effective concentration 50) values of Daphnia magna after 24 and 48 hours in non-genetically modified rice (Ilmi) and Bt-transgenic (Bt-9) rice.
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