Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Gomisin G Suppresses the Growth of Colon Cancer Cells by Attenuation of AKT Phosphorylation and Arrest of Cell Cycle Progression

  • Maharjan, Sony (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Park, Byoung Kwon (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Lee, Su In (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Lim, Yoongho (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Lee, Keunwook (Department of Biomedical Science, College of Natural Science, Hallym University) ;
  • Lee, Younghee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Kwon, Hyung-Joo (Center for Medical Science Research, College of Medicine, Hallym University)
  • Received : 2018.03.26
  • Accepted : 2018.05.17
  • Published : 2019.03.01
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Abstract

Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.

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References

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