DOI QR코드

DOI QR Code

Effects of Rutin on Anti-inflammatory in Adipocyte 3T3-L1 and Colon Cancer Cell SW-480

지방세포 3T3-L1과 대장암세포 SW-480에서 메밀 성분인 rutin의 항염증 효과

  • Lee, Suenglim (Department of Food and Cooking, Sangji Youngseo College) ;
  • Seo, Eunyoung (Department of Food and Nutrition, Jangan University)
  • 이승림 (상지영서대학교 식품조리과) ;
  • 서은영 (장안대학교 식품영양과)
  • Received : 2018.12.05
  • Accepted : 2019.02.21
  • Published : 2019.02.28

Abstract

Purpose: The objective of this study was conducted to investigate the effects of rutin, buckwheat components on cell growth and anti-inflammation in adipocyte 3T3-L1 and human colon cancer cell SW-480. Methods: We cultured 3T3-L1 adipocyte and SW-480 colon cancer cell to confluence, at which time starvation was induced with SFM for 1 day. Cells were then cultured in medium containing 0, 25, 50, or $100{\mu}mol/mL$ of rutin 3T3-L1 or 0, 10, 20, or $40{\mu}mol/mL$ SW-480. Cell viability was measured using a cell viability kit. In addition, we examined the expression of mRNA related to inflammation. RT-PCR was used to quantity tumor necrosis factor ($TNF-{\alpha}$), interleukin-$1{\beta}$ ($IL-1{\beta}$), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA levels. Results: Rutin significantly inhibited 3T3-L1 and SW-480 cell proliferation in a dose and time dependent manner. Rutin also significantly reduced the mRNA expression of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ at the highest dose. In addition, rutin treatment caused a significant reduction in COX-2 and iNOS mRNA levels compared to the control group. Conclusion: Overall, our results suggest that rutin has the potential to reduce inflammation, and that these effects are greater during tissue-damaging inflammatory conditions.

Keywords

SSMHB4_2019_v34n1_84_f0001.png 이미지

Effects of rutin on cell proliferation in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0002.png 이미지

Effects of rutin on IL-1β mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0003.png 이미지

Effects of rutin on IL-6 mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0004.png 이미지

Effects of rutin on TNF-α mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0005.png 이미지

Effects of rutin on Cox-2 mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0006.png 이미지

Effects of rutin on iNOS mRNA expression in 3T3-L1 and SW-480 cells.

Primer sequences used for realtime PCR

SSMHB4_2019_v34n1_84_t0001.png 이미지

References

  1. Artwohl M, Muth K, Kosulin K, de Martin R, Holzenbein T, Rainer G, Freudenthaler A, Huttary N, Schmetterer L, Waldhäusl WK, Baumgartner-Parzer SM. 2007. R-(+)- alpha-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1. Am J Physiol Endocrinol Metab., 293(3):E681-689 https://doi.org/10.1152/ajpendo.00584.2006
  2. Asfour MH, Mohsen AM. 2018. Formulation and evaluation of pH-sensitive rutin nanospheres against colon carcinoma using HCT-116 cell line. J Advanced Res., 9:17-26 https://doi.org/10.1016/j.jare.2017.10.003
  3. Breithaupt-Grögler K, Niebch G, Schneider E, Erb K, Hermann R, Blume HH, Schug BS, Belz GG. 1999. Doseproportionality of oral thioctic acid-coincidence of assessments via pooled plasma and individual data. Eur J Pharm Sci., 8(1):57-65 https://doi.org/10.1016/S0928-0987(98)00061-X
  4. Brooks GA, Casey MD, Timothy PW. 1984. Estimation of anaerobic energy production and efficiency in rat during exercise. J Appl Physiol, 56:520-525 https://doi.org/10.1152/jappl.1984.56.2.520
  5. Cho Y, Choi MY. 2015. Inhibitory effects of flavonoids on growth of HT-29 human colon cancer cells. J korean Soc Food Sci and Nutri., 44(3):338-346 https://doi.org/10.3746/jkfn.2015.44.3.338
  6. Chu SK, Seo EY, Kim WK, Kang NE. 2008. Effect of cyanidin on cell motility and invasion in MDA-MB-231 human breast cancer cells. Korean J Nutr., 41(8): 711-717
  7. Cui CB, Lee EY, Ham SS, Lee DS. 2008. Antimutagenic and cytotoxic effects of an ethanol extract of buckwheat sprout. J Korean Soc. Appl. Biol. Chem., 51(3):212-218
  8. Davis GDJ, Masilamoni JG, Arul V, Sakthivelu IV, Kumar MSM, Baraneedharan U, Paul SFD. 2009. Radioprotective effect of DL-${\alpha}$-lipoic acid on mice skin fibroblasts. Cell Biol Toxicol., 25:331-340 https://doi.org/10.1007/s10565-008-9087-5
  9. Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE. 1998. Cyclooxygenase in biology and disease. FASEB J., 12:1063-1073 https://doi.org/10.1096/fasebj.12.12.1063
  10. Ghibu S, Richar C, Delemasure S, Vergely C, Mogosan C, Muresan A. 2008. An endogenous dithiol with antioxidant properties: Alpha-lipoic acid, potential uses in cardiovascular disease. Elsevier Masson SAS., 57:161-165
  11. Grabowski PS, Wright PK, Van't Hof RJ, Helfrich MH, Ohshima H, Ralston SH. 1997. Immunolovalization of inducible nitric oxide synthase in synovium and cartilage in rheumatoid arthritis and osteoarthritis. Br J Rheumatol., 36:651-655 https://doi.org/10.1093/rheumatology/36.6.651
  12. Green H, Kehinde O. 1975. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell., 5(1):19-27 https://doi.org/10.1016/0092-8674(75)90087-2
  13. Griffith JQ, Couch JF, Lindauer MA. 1995. Effect of rutin on increased capillary fragility in man. Proc. Soc. Exp. Bio. Med., 55:228-229 https://doi.org/10.3181/00379727-55-14532
  14. Hahm JR, Noh HS, Ha JH, Roh GS, Kim DR. 2014. Alpha-lipoic acid attenuates adipocyte differentiation and lipid accumulation in 3T3-L1 cells vi dependent autophagy. Life science., 100(2):124-132
  15. Han D, Sen CK, Roy S, Kobayashi MS, Tritschler HJ, Packer L. 1997. Protection against glutamate-induced cytotoxicity in C6 glial cells by thiol antioxidants. Am J Physiol., 273: R1771-R1778
  16. Hirsch J, Batchelor B. 1976. Adipose tissue cellularity in human obesity. Clin Endocrinol Metab., 5(2):299-311 https://doi.org/10.1016/S0300-595X(76)80023-0
  17. Hwang EJ, Lee SY, Kwan SJ, Park MH, Bu HO. 2006. Antioxidative, antimicrobial and cytotoxic activities of fagopyrum esculentum moench extract in germinate. Korean J Med Cor Sci.,14(1):1-7
  18. Jin HJ, Kim JS, Kang SS, Son KH, Chang HW, Kim HP. 2010. Anti-inflammatory and anti-arthritic activity of the roots of Sophora flavescens. J Ethnopharmacol., 127(3):589-295 https://doi.org/10.1016/j.jep.2009.12.020
  19. Jordan SW, Cronan JE Jr. 1997. Biosynthesis of lipoic acid and posttranslational modification with lipoic acid in Escherichia coli. Methods Enzymol., 279:176-183 https://doi.org/10.1016/S0076-6879(97)79021-9
  20. Kang RY, Freire-Moar J, Sigal E, Chu CQ, 1996. Expression of cyclooxygenase-2 in human and an animal model of rheumatoid arthritis. Br J Rheumatol., 35:711-718 https://doi.org/10.1093/rheumatology/35.8.711
  21. Kim DM, Ahn CW, Nam SY. 2005. Prevalence of obesity in Korea. Obes Rev., 6(2):117-121 https://doi.org/10.1111/j.1467-789X.2005.00173.x
  22. Kim MS, Park JY, Namkoong C, Jang PG. 2004. Anti-obesity effects of alpha-lipoic acid mediated by suppression of hypothalamic AMP-activated protein kinase. Nature medicine., 10(7):727-733 https://doi.org/10.1038/nm1061
  23. Koh JM, Lee YS, Byun CH, Chang EJ, Kim H, Kim YH, Kim HH, Kim GS. 2005. Alpha-lipoic acid suppresses osteoclastogenesis despite increasing the receptor activator of nuclear factor kappaB ligand/osteoprotegerin ratio in human bone marrow stromal cells. J Endocrinol. 185(3):401-413 https://doi.org/10.1677/joe.1.05995
  24. Kundu JK, Surh YJ. 2008. Inflammaton: gearing the journey to cancer. Mutat Res., 659(1-2):15-30 https://doi.org/10.1016/j.mrrev.2008.03.002
  25. Lagathu C, Bastard JP, Aclair M, Macchi M, Capeau J, Caron M. 2003. Chronic interleukin-6(IL-6) treatment increased IL-6 secretion and induced insulin resistence in adipocyte: prevention by rosiglitazone. Biochem Biophys Res Commun., 311:372-379 https://doi.org/10.1016/j.bbrc.2003.10.013
  26. Lee HH, 2008. A study on the utilization of functional cosmetics materials using the bioactive compounds from Buckwheat. Kor. J. Aesthet. Cosmetol., 6(2):1-7
  27. Lee MS, Kim CT, Kim CJ, Cho YJ, Kim YH. 2006. Efeects of Portulaca Oleracea L. Extract on lipolysis and hormone sensitive lipase (HSL) gene expression in 3T3-L1 adipocytes. Korean J Nutr., 39(8):742-747
  28. Lee WJ, Lee IK, Kim HS, Kim YM, Koh EH, Won JC, Han SM, Kim MS, Jo I, Oh GT, Park IS, Youn JH, Park SW, Lee KU, Park JY. 2005. Alpha-lipoic acid prevents endothelial dysfunction in obese rats via activation of AMP-activated protein kinase. Arterioscler Thromb Vasc Biol., 25(12):2488-2494 https://doi.org/10.1161/01.ATV.0000190667.33224.4c
  29. Lumeng CN, Bodzin JL, Saltiel AR. 2007. Obesity induces a phenotypic switch in adipose tissue macrophage polarization. J Clin invest., 117:175-184 https://doi.org/10.1172/JCI29881
  30. Maliska D, Winiarska K, 2005. Lipoic acid: characteristic and therapeutic application. Postepy Hig Med Dosw., 59:535-543
  31. Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Thomas P, Dannenberg AJ. 2003. Curcumin prevents alcoholinduced liver disease in rats by inhibiting the expression of NF-kappaB-dependent genes. Am J Physiol Gastointest Liver Physiol., 284:G321-327 https://doi.org/10.1152/ajpgi.00230.2002
  32. Ouchi N, Kihara S, Funahashi T, Matsuzawa Y, Walsh K. 2003. Obesity, adiponectin and vascular inflammatory disease. Curr Opin Lipid 14:561-566 https://doi.org/10.1097/00041433-200312000-00003
  33. Packer L, Witt EH, Tritschler HJ. 1995. Alpha-lipoic acid as a biological antioxidant. Free Radic Biol Med., 19:227-250 https://doi.org/10.1016/0891-5849(95)00017-R
  34. Pari L, Murugavel P. 2004. Protective effect of alpha lipoic acid against chloroquine-induced hepatotoxicity in rats. J Appl Toxicol., 24:21-26 https://doi.org/10.1002/jat.940
  35. Park HS, Choi JS, 1997. Effects of dietary fat and fiber on biomarkers of colon cancinogenesis in rats. Korean Society for lipid and Atherosclerosis., 7(2):103-124
  36. Park SK, Jeen YT, Lee KG, Kim JH, Hyun JJ, Kim ES, Park SH, Keum BR, Seo YS, Kim YS, Chun HJ, Um SH, Choi JH, Kim CD. 2009. Clinical significance of serum C-reactive protein in patients with cololectal cancer. Intest Res., 7(2):93-99
  37. Prieto-Hontoria PL, Fernandez-Galilea M, Perez-Matute P, Martinez JA, Moreno-Aliaga M. 2013 Lipoic acid inhibits adiponectin production in 3T3-L1 adipocyte. J Physiol and Biochem., 69(3):595-600 https://doi.org/10.1007/s13105-012-0230-7
  38. Reed LJ. 1957. The chemistry and function of lipoic acid. Adv Enzymol Relat Subj Biochem., 18:319-347
  39. Reinecker HC, Steffen M, Witthoeft T, Pflueger I, Schreiber S, MacDermott RP, Readler A. 1993. Enhanced secretion of tumor necrosis factor-${\alpha}$, IL-6, and IL-1${\beta}$ by isolate Lamina propria mononuclear cells from patients with ulcerative colitis and Crohn's disease. Clin Exp Immunol., 94:174-181 https://doi.org/10.1111/j.1365-2249.1993.tb05997.x
  40. Rosenberg HR, Culik R. 1959. Effect of alpha-lipoic acid on vitamin C and vitamin E deficiencies. Arch Biochem Biophys., 80-86
  41. Saengsirisuwan V, Perez FR, Sloniger JA, Maier T, Henriksen EJ. 2004. Interactions of exercise training and alpha-lipoic acid on insulin signaling in skeletal muscle of obese Zucker rats. Am J Physiol Endocrinol Metab., 287(3):E529-536 https://doi.org/10.1152/ajpendo.00013.2004
  42. Seo EY. 2015. Effects of (6)-gingerol, ginger component on adipocyte development and differentiation in 3T3-L1. J Nutri Health., 48(4):327-334 https://doi.org/10.4163/jnh.2015.48.4.327
  43. Storck M, Schilling M, Burkhardt K, Prestel R, Abendroth D, Hammer C. 1994. Production of proinflammatory cytokine and adhesion molecules in ex-vivo xenogeneic kidney perfusion. Transpl Int., 7(1):647-649 https://doi.org/10.1111/j.1432-2277.1994.tb01464.x
  44. Teichert J, Kern J, Tritschler HJ, Ulrich H, Preiss R. 1998. Investigations on the pharmacokinetics of alpha-lipoic acid in healthy volunteers. Int J Clin Pharmacol Ther., 36(12):625-628
  45. Terzie J, Grivennikov S, Karin M. 2010. Inflammation and colon cancer. Castroenterology., 136(6):2101-2114.e5
  46. Tilg H, Moschen AR. 2008. Inflammatory mechanisms in the regulation of insulin resistence. Mol Med., 14:222-231 https://doi.org/10.2119/2007-00119.Tilg
  47. Uysal KT, Wiesbrock SM, Hotamisligil GS. 1998. Functional analysis of tumor necrosis factor (TNF) receptor in TNFalpha-mediated insulin resistance in genetic obesity. Endocrinology., 139:4832-4838 https://doi.org/10.1210/endo.139.12.6337
  48. Wada H, Shintani D, Ohlrogge J. 1997. Why do mitochondria synthesize fatty acids? Evidence for involvement in lipoic acid production. Proc Natl Acad Sci USA., 18;94(4):1591-1596 https://doi.org/10.1073/pnas.94.4.1591
  49. Weisberg SP. 2006. CCCR2 modulates inflammatory and metabolic effects of high-fat feeding. J Clin Invest., 116:115-124 https://doi.org/10.1172/JCI24335
  50. Yeh WC, Cao Z, Classon SL, McKnight SL. 1995. Cascade regulation of terminal adipocyte differentiation by three member of the C/EBP family of leucin zipper proteins. Gene., 9:168-181 https://doi.org/10.1101/gad.9.2.168
  51. Yi X, Pashai A, Xia M, Moreau R. 2013. Reveral of obesityinduced hypertriglyceridemia by (R)-${\alpha}$-lipoic acid in ZDF (fa/fa) rats. Biochem Biophys Res Commun., 439(3):390-395 https://doi.org/10.1016/j.bbrc.2013.08.063
(34141) Korea Institute of Science and Technology Information, 245, Daehak-ro, Yuseong-gu, Daejeon
Copyright (C) KISTI. All Rights Reserved.