한국식생활문화학회지 (Journal of the Korean Society of Food Culture)

  • 제34권1호
  • /
  • Pages.84-92
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  • 2019
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  • 1225-7060(pISSN)
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  • 2288-7148(eISSN)
한국식생활문화학회 (Korean Society of Food Culture)
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지방세포 3T3-L1과 대장암세포 SW-480에서 메밀 성분인 rutin의 항염증 효과

Effects of Rutin on Anti-inflammatory in Adipocyte 3T3-L1 and Colon Cancer Cell SW-480

  • 이승림 (상지영서대학교 식품조리과) ;
  • 서은영 (장안대학교 식품영양과)
  • Lee, Suenglim (Department of Food and Cooking, Sangji Youngseo College) ;
  • Seo, Eunyoung (Department of Food and Nutrition, Jangan University)
  • 투고 : 2018.12.05
  • 심사 : 2019.02.21
  • 발행 : 2019.02.28
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초록

Purpose: The objective of this study was conducted to investigate the effects of rutin, buckwheat components on cell growth and anti-inflammation in adipocyte 3T3-L1 and human colon cancer cell SW-480. Methods: We cultured 3T3-L1 adipocyte and SW-480 colon cancer cell to confluence, at which time starvation was induced with SFM for 1 day. Cells were then cultured in medium containing 0, 25, 50, or $100{\mu}mol/mL$ of rutin 3T3-L1 or 0, 10, 20, or $40{\mu}mol/mL$ SW-480. Cell viability was measured using a cell viability kit. In addition, we examined the expression of mRNA related to inflammation. RT-PCR was used to quantity tumor necrosis factor ($TNF-{\alpha}$), interleukin-$1{\beta}$ ($IL-1{\beta}$), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA levels. Results: Rutin significantly inhibited 3T3-L1 and SW-480 cell proliferation in a dose and time dependent manner. Rutin also significantly reduced the mRNA expression of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ at the highest dose. In addition, rutin treatment caused a significant reduction in COX-2 and iNOS mRNA levels compared to the control group. Conclusion: Overall, our results suggest that rutin has the potential to reduce inflammation, and that these effects are greater during tissue-damaging inflammatory conditions.

키워드

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Effects of rutin on cell proliferation in 3T3-L1 and SW-480 cells.

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Effects of rutin on IL-1β mRNA expression in 3T3-L1 and SW-480 cells.

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Effects of rutin on IL-6 mRNA expression in 3T3-L1 and SW-480 cells.

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Effects of rutin on TNF-α mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0005.png 이미지

Effects of rutin on Cox-2 mRNA expression in 3T3-L1 and SW-480 cells.

SSMHB4_2019_v34n1_84_f0006.png 이미지

Effects of rutin on iNOS mRNA expression in 3T3-L1 and SW-480 cells.

Primer sequences used for realtime PCR

SSMHB4_2019_v34n1_84_t0001.png 이미지

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