Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of exercise on myokine gene expression in horse skeletal muscles

  • Lee, Hyo Gun (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Choi, Jae-Young (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Park, Jung-Woong (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Park, Tae Sub (Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National, University) ;
  • Shin, Donghyun (Department of Animal Biotechnology, Chonbuk National, University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
  • Received : 2018.05.16
  • Accepted : 2018.09.13
  • Published : 2019.03.01
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Abstract

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

Keywords

References

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